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Compound probiotics preparation with gastric and intestinal mucosa protection effect, and application of compound probiotics preparation

A technology of compound bacterial agent and Lactobacillus, applied in the field of microbiology, can solve problems such as intestinal epithelial erosion and damage to the integrity of the mucosal barrier

Active Publication Date: 2020-01-03
GUANGDONG YIKEWEI BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The DSS model is very similar to human ulcerative colitis in terms of morphology and symptoms. The modeling mechanism of DSS is related to the direct toxic effect of DSS drugs on the intestinal epithelium, which can cause intestinal epithelial erosion and eventually lead to the integrity of the mucosal barrier. to destroy

Method used

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  • Compound probiotics preparation with gastric and intestinal mucosa protection effect, and application of compound probiotics preparation
  • Compound probiotics preparation with gastric and intestinal mucosa protection effect, and application of compound probiotics preparation
  • Compound probiotics preparation with gastric and intestinal mucosa protection effect, and application of compound probiotics preparation

Examples

Experimental program
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Effect test

preparation example

[0039] Preparation of experimental materials (preparation example) 1:

[0040] The preparation of the three strains of viable bacterial suspension: the three strains of bacteria W68, K9, and FEED8 were respectively prepared according to the following conditions, 10 7 The inoculum amount of CUF / ml was inoculated in 500ml of MRS liquid medium, cultured anaerobically at 37°C for 12h, centrifuged at 4500r / min for 15min to collect the bacteria, and the sediment was resuspended in skim milk with a mass fraction of 10%, and prepared respectively into 2×10 6 Low-dose bacterial suspension of CFU / ml, marked as W1, K1, F1. 2×10 8 Medium-dose bacterial suspension of CFU / ml, marked as W2, K2, F2. 2×10 10 The high-dose bacterial suspension of CFU / ml is marked as W3, K3, and F3.

[0041] Preparation of dead bacteria suspensions of three strains: three strains of strains W68, K9, and FEED8 were respectively prepared according to 10 7 The inoculum amount of CUF / ml was inoculated in 100ml o...

Embodiment 1-12

[0045] Examples 1-12 are used to illustrate that Bifidobacterium longum W68, Lactobacillus paracasei K9, and Lactobacillus plantarum FEED8 of the present invention have different concentrations of single live bacteria and single dead bacteria all have gastric mucosa protection effect.

[0046] The experimental rats were fed with 8 rats per cage, freely ingested feed and water, and adaptively fed for 1 week. According to body weight, they were randomly divided into 12 groups with 8 rats in each group. Bifidobacterium-free and Lactobacillus-free rat feed was purchased from Shanghai Slack Experimental Animal Co., Ltd., and sterilized by radiation before feeding. After the grouped rats were weighed, they were fed with the bacterial suspensions W1, K1, F1, W2, K2, F2, W3, K3, F3, W4, K4, and F4 in Preparation Example 1, respectively. The amount of gavage is 0.1ml / kg / d rat body weight. Continuous feeding for 30d. During the experiment, the rats were free to eat and drink, and had...

preparation example )2

[0061] (1) Preparation of three live bacterial suspensions of three strains of bacteria: three strains of bacteria W68, K9, and FEED8 were prepared according to 10 7 The inoculum amount of CUF / ml was respectively inoculated in 500ml of MRS liquid medium, cultured anaerobically at 37°C for 12h, centrifuged at 4500r / min for 15min to collect the bacteria, and the bacteria pellet was resuspended in skim milk with a mass fraction of 10%, and prepared respectively into 2×10 8 The dose of CFU / ml bacterial suspension, W68 and K9 are mixed according to the mass ratio of 1:1 to prepare the bacterial suspension A1, W68 and FEED8 are mixed according to the mass ratio of 1:1 to prepare the bacterial suspension A2, FEED8 and K9 are prepared according to the mass ratio of 1 :1 mixed to prepare bacterial suspension A3, W68:K9:FEED8=33:33:34 mixed according to mass ratio to prepare bacterial suspension A4.

[0062] (2) Preparation of dead bacteria suspensions of three strains: Three strains o...

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Abstract

The invention relates to the technical field of microorganisms, and discloses a compound bacterium agent. The compound bacterium agent is prepared from at least two of bifidobacterium longum, lactobacillus paracasei and lactobacillus plantarum. Compared with a model control group, through the compound bacterium agent, the rat gastric mucosa bleeding belt area can be significantly decreased, the gastric mucosa blood flow is increased, and the gastric mucosa damage index is decreased. The mouse colon length is significantly increased, the mouse colon tissue pathology score is decreased, and ulcerative colitis caused by intestinal mucosa damage of mice is relieved. Therefore, the probiotics compound bacterium agent has a certain gastric and intestinal mucosa protection effect, and is very suitable for preparation of food, health care products, medicines and food supplements.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a compound probiotic preparation with protective effect on gastrointestinal mucosa and application thereof. Background technique [0002] Gastrointestinal tract is an important organ in the human digestive system, the source of the body's power, and occupies an irreplaceable important position in the body's substance metabolism. The inner wall of the gastrointestinal tract has a layer of mucosal tissue, which acts as a natural barrier and is of great significance to the protection of the gastrointestinal tract. But the mucous membrane itself is very fragile, coupled with external factors such as environment, diet, emotional changes, and rhythm of life, it is easy to damage it. Long-term damage can lead to acute and chronic gastroenteritis, ulcers, and even tumors. And my country is a big country for gastroenteropathy, and the course of disease is longer, is recurrent, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A23L2/39A23L2/02A23L2/52A23C9/123A23G9/36A23G3/36A61K35/745A61K35/747A61P1/00A61P3/02C12R1/01C12R1/225C12R1/25
CPCC12N1/20A23L33/135A23L2/39A23L2/02A23L2/52A23C9/1234A23G9/363A23G3/366A61K35/745A61K35/747A61P1/00A61P3/02A23V2002/00A23V2400/165A23V2400/533A23V2400/169A23V2250/628A23V2250/032A23V2250/51088A23V2250/5066A23V2250/264A23V2250/5114A23V2250/28A23V2250/5062A61K2300/00
Inventor 闫刘慧金庭飞王然张伟
Owner GUANGDONG YIKEWEI BIOLOGICAL TECH CO LTD
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