ag based + and hg 2+ An Electrochemical Detection Method for the Etching of DNA Quadruplets
An electrochemical, quadruple technology, applied in the field of functional biomaterials and biosensing, to achieve the effect of good selectivity, accurate results and low cost
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Embodiment 1
[0024] Embodiment 1 Preparation of sensor
[0025] (1) Gold electrode (Au): Al with a diameter of 0.3 μm is used before the gold electrode is used 2 o 3 The powder was ground, ultrasonically cleaned 3 times with ultrapure water, dried in a nitrogen stream, and soaked in H 2 SO 4 (0.5M) solution, carry out cyclic voltammetry scanning in the range of -0.5V ~ +1.5V for 10min, and finally wash with ultrapure water and blow dry with nitrogen gas for later use.
[0026](2) Electrode 1: Take a DNA solution (0.1 μM, 5 μL), drop-coat it on the surface of Au, and incubate overnight in a refrigerator at 4°C. Slowly wash the electrode with distilled water, treat with 1.0mM mercaptohexanol (MCH) for 30min, and wash slowly with distilled water.
[0027] (3) Electrode 2: Mix 2 μL distilled water, 1 μL TdT buffer, dNTP (0.4 μL dTTP+0.6 μL dGTP, 10 mM), TdT (1 μL, 10 U / mL), mix well, drop on the surface of electrode 1, at 37 ° C After standing for 1 h, the electrode was slowly washed with...
Embodiment 2
[0029] Example 2 Ag + Feasibility experiment for testing
[0030] Preparation of electrode 3-1: Prepare a modified electrode as in Example 1, then take out the electrode and place it in 2nM Ag + Incubate in the solution for 30 minutes, place in PBS solution and scan the response of the electrode to the solution by cyclic voltammetry;
[0031] The result is as figure 2 , adding Ag + Afterwards, the electrochemical response was greatly weakened, proving that the sensor can be used to detect Ag + , also proved that Ag + Etching of G-quadruplexes.
Embodiment 3
[0032] Example 3 Different concentrations of Ag + Effect on electrochemical signal
[0033] Subsequently, different concentrations of Ag were introduced + , Ag + Concentrations range from 0.001 to 10 nM. The experimental results show that, as shown in Figure 3, it shows that with Ag + As the concentration increases, the sensor’s response to Ag + The weaker the electrochemical response; the sensor for Ag + The linear correlation equation of the current response to the concentration y=19.95x-42.53, R 2 =0.9980, the linear range is 0.001~2nM, according to S / N calculation, the detection limit is 0.52pM. Description of sensor for Ag + High-sensitivity detection is possible.
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