Novel microbial strain for pholiota and artificial cultivation method and application of novel microbial strain

A technology of artificial cultivation and parasol, applied in the directions of botanical equipment and methods, microorganism-based methods, biochemical equipment and methods, etc., can solve problems such as unfavorable taxonomy and development of the genus Cymbidium, and achieve a significant inhibition rate and a strong inhibition rate. , nutrient-rich effect

Active Publication Date: 2020-01-07
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
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Problems solved by technology

Bas (1971) disagreed with Smith and Hesler's point of view, thinking that they limited the scope of the genus too broadly, and the wider the range, the greater the variation of characteristics, thus separating the relatively unimportant genera related to Phycocystis, This is not conducive to the development of the taxonomy of the genus

Method used

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  • Novel microbial strain for pholiota and artificial cultivation method and application of novel microbial strain
  • Novel microbial strain for pholiota and artificial cultivation method and application of novel microbial strain
  • Novel microbial strain for pholiota and artificial cultivation method and application of novel microbial strain

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Experimental program
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Effect test

Embodiment 1

[0072] Embodiment 1: New strain identification

[0073] On March 22, 2014, Hu Huiping, Liu Yuanchao and others conducted large-scale fungal resource collection and investigation in Chebaling National Nature Reserve, Guangdong. figure 1 as shown, figure 2 and image 3 Its microstructure diagram, and the original strain obtained from tissue isolation, named Pholiota sp. HMGIM-W140054, was preserved in the China Center for Type Culture Collection (CCTCC for short, address For: Wuhan, China), the deposit number is CCTCC NO: M 2019414.

[0074] Since there was no species of the genus Phytophthora consistent with its description in terms of macroscopic and microscopic morphology, in order to further confirm its classification, its DNA was extracted and its multi-segment genes were sequenced, including the fungal ribosomal intergenic region ITS, ribosomal large subunit The base LSU, encoding the second largest subunit rpb2 of RNA polymerase II, etc. are multigene fragments used i...

Embodiment 2

[0088] Embodiment 2 artificial cultivation

[0089] One, culture medium (by weight percentage):

[0090] 1. Tissue separation medium (comprehensive PDA):

[0091] Potato 20%, glucose 2%, agar 2%, potassium dihydrogen phosphate 0.3%, magnesium sulfate 0.15%, vitamin B1 trace, and the rest is water.

[0092] 2. Purified mother seed medium (Red Bengal medium):

[0093] 0.5% peptone, 1% glucose, 0.1% potassium dihydrogen phosphate, magnesium sulfate (MgSO4 7H 2 O) 0.05%, agar 2%, 1 / 3000 Bengal red solution 10%, chloramphenicol 0.01%, and the rest is water.

[0094] 3. Production medium of mother species (enriched with comprehensive PDA):

[0095] Potato 20%, glucose 2%, peptone 1%, agar 2%, potassium dihydrogen phosphate 0.3%, magnesium sulfate 0.15%, vitamin B1 trace, and the rest is water.

[0096] 4. Production medium:

[0097] 98-99% sorghum, 1-2% calcium carbonate.

[0098] 5. Cultivation material:

[0099] 31% cottonseed hulls, 58% wood chips, 10% bran, 1% CaCO 3 ;T...

Embodiment 3

[0125] Embodiment 3 fermented liquid antibacterial activity assay

[0126] 1. Method

[0127] 1. Preparation:

[0128] Culture medium for bacteria: nutrient agar / broth medium;

[0129] Nutrient agar / broth medium: beef extract 3g, peptone 10g, sodium chloride 5g, agar 15g, add water to 1000ml to adjust pH to 7.4 (no agar in nutrient broth).

[0130] The preparation of test sample ethyl acetate extract: use rice culture medium (prescription is rice+water), insert bacterial strain of the present invention and cultivate for 45 days, add ethyl acetate, ultrasonic extraction 2 times, 40min / time, merge extract , recover the solvent in a rotary evaporator, and transfer it to a collection bottle when the extract is about 1-2 mL by rotary evaporation, and place the ethyl acetate extract in a vacuum desiccator. Sterilize with a sterile syringe and a membrane filter for use.

[0131] Positive control preparation: ampicillin cryopreservation solution, diluted with sterile water to a re...

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Abstract

The invention relates to a novel microbial strain of valuable and rare edible fungus and an artificial cultivation method and application of the novel microbial strain, in particular to a novel microbial strain Pholiotasp. for pholiota and the artificial cultivation method and application of the novel microbial strain. The novel microbial strain for the pholiota is derived from a chebaling national nature reserve of Guangdong, is the novel microbial strain for the pholiota through identification, tissue isolation is carried out to obtain an original microbial strain which is named (pholiota sp.)HMGIM-W140054, is preserved in China Center For Type Culture Collection (called CCTCC for short, the address is Wuhan, China) at third June, 2019, and the preservation number is CCTCC NO: M 2019414.According to the novel microbial strain, artificial domesticated cultivation is realized, more strong and significant inhibition ratio is shown to staphylococcus, high histidine content, crude polysaccharide content and potassium content are achieved, and the novel microbial strain is a novel strain with rich nutritional ingredients, high cultivation yield and development prospects.

Description

technical field [0001] The invention relates to a new strain of rare edible fungus and its artificial cultivation method and use, in particular to a new strain of the genus Phytophthora and its artificial cultivation method and use. Background technique [0002] At present, the industry of edible and medicinal fungi is developing rapidly. According to statistics from the China Edible Fungi Association, the output of edible and medicinal fungi in my country reached 37.12 million tons in 2017, an increase of 3.21% over 2016, and the output value was 272.192 billion yuan. %, more than 20 million employees, and the edible fungus industry ranks fifth in the planting industry after grain, vegetables, fruit, and oil, surpassing tea and sericulture. [0003] Today, with the vigorous development of the edible and medicinal mushroom industry, more and more rare edible and medicinal mushroom varieties have gradually entered people's field of vision. Morels etc. However, there are also...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A23L31/00A23L33/00A61K36/07A61P31/04C12R1/645
CPCA23V2002/00A61K36/07A23L31/00A23L33/00A61P31/04C12N1/14C12N1/145C12R2001/645A23V2200/30A23V2250/208A01H15/00A23L33/10A01H1/12A23L33/40
Inventor 胡惠萍刘远超梁晓薇吴清平谢意珍张智
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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