Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for preparing anthocyanin from lonicera caerulea fruits

A technology of indigo fruit and anthocyanin, which is applied in chemical instruments and methods, organic chemistry, organic dyes, etc., can solve the problems of high proportion of fat-soluble impurities, health hazards of operators, increase of anthocyanin cost, etc., and achieve simple operation , improve the safety of use and food, and save resources

Pending Publication Date: 2020-01-10
DAXINGANLING PERFECTION NOBILITY FRIGID ZONE BIO TECH CO LTD
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The advantage of the organic solvent extraction method is that the extraction equipment is simple and easy to operate; the disadvantage is that the organic solvent is easy to consume, time-consuming, easy to cause high solvent residue in the product, and cause pollution to the environment
Microwave and ultrasonic methods may cause great harm to the health of operators due to equipment factors
Supercritical extraction can be industrialized at present, but because this technology converts gaseous carbon dioxide into liquid carbon dioxide, and liquid carbon dioxide has a stronger ability to extract fat-soluble substances, resulting in a higher proportion of fat-soluble impurities in the product, which is extremely Greatly increased the cost of producing anthocyanins
[0005] At present, the more commonly used purification method is mainly carried out by using macroporous adsorption resin. The advantage of macroporous adsorption resin is that it has a large adsorption capacity and can be used repeatedly; the disadvantage is that a large amount of ethanol is required for elution during the purification process, and the loss of solvent is large. , increase production cost

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take 1 kg of fresh blue indigo fruit and put it into a colloid mill to crush and beat it, then add 1 kg of purified water and stir evenly, extract at a pressure of 16 MPa and a temperature of 300 ° C for 30 minutes, centrifuge the feed liquid, and collect the centrifuged liquid to the storage tank , the slag was extracted for the second time. The weight of EGCG added in each extraction process is 0.4g. Combine 2 times of centrifugation solution, let it stand at room temperature, then release the lower layer of water layer, and filter the water layer to obtain filtrate A, then place the filtrate A in a refrigerator at 2°C for 6 hours, and filter again to obtain filtrate B . The filtrate B is finely filtered with a ceramic membrane. The pore size of the ceramic membrane is a membrane element of 100nm. The temperature of the treatment liquid is 21°C and the pressure is 0.4MPa. The permeated liquid of the ceramic membrane is collected. Then the ceramic membrane permeated ...

Embodiment 2

[0026] Take 1 kg of fresh blue indigo fruit and put it into a colloid mill to crush and beat it, then add 2 kg of purified water and stir evenly, extract at a pressure of 18 MPa and a temperature of 320 ° C for 20 minutes, centrifuge the feed liquid with a centrifuge, and collect the centrifuged liquid to a storage tank , the slag was extracted for the second time. The weight of EGCG added in each extraction process is 0.3g. Combine 2 centrifugates, put them at room temperature to separate layers, release the lower water layer, and filter the water layer to obtain filtrate A, then place the filtrate A in a refrigerator at 5°C for winterization for 8 hours, and filter again to obtain filtrate B . The filtrate B is finely filtered with a ceramic membrane. The pore size of the ceramic membrane is a membrane element of 100nm. The temperature of the treatment liquid is 23°C and the pressure is 0.3MPa. The ceramic membrane permeate is collected. Then the ceramic membrane permeated...

Embodiment 3

[0028] Take 1 kg of fresh blue indigo fruit and put it into a colloid mill to crush and beat it, then add 3 kg of purified water and stir evenly, extract at a pressure of 20 MPa and a temperature of 340 ° C for 15 min, centrifuge the feed liquid, and collect the centrifuged liquid to the storage tank , the slag was extracted for the second time. The weight of EGCG added in each extraction process is 0.5g. Combine 2 centrifugates, put them at room temperature, release the lower water layer after stratification, and filter the water layer to obtain filtrate A, then place the filtrate A in a refrigerator at 1°C for winterization for 10 hours, and filter again to obtain filtrate B . The filtrate B is finely filtered with a ceramic membrane. The pore size of the ceramic membrane is a membrane element of 100nm. The temperature of the treatment liquid is 25°C and the pressure is 0.2Mpa. The ceramic membrane permeate is collected. Then the ceramic membrane permeate was concentrated ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of natural organic chemistry. The invention discloses a method for preparing anthocyanin from lonicera caerulea fruits. The method comprises the following steps: crushing and pulping fresh lonicera caerulea fruits by adopting a colloid mill, and extracting the crushed and pulped lonicera caerulea fruits by utilizing a subcritical water extraction technology in the presence of an antioxidant; centrifuging and storing an obtained centrifugate at room temperature for layering, collecting and filtering a lower water layer, carrying out winterization and filtering, treating an obtained filtrate by adopting a ceramic membrane and a nanofiltration membrane in sequence, and finally obtaining an anthocyanin finished product by adopting molecular distillation and vacuum freeze-drying technologies. The anthocyanin product prepared by the method has the advantages of no solvent residue, low cost, no environmental pollution, high use and eating safety and the like.

Description

technical field [0001] The invention belongs to the field of natural organic chemistry, and relates to a method for preparing anthocyanins by using cyanidome fruit as a raw material. In particular, it relates to a method for preparing anthocyanins by subcritical water technology extraction, winterization and membrane technology purification. Background technique [0002] Anthocyanin is a water-soluble pigment that can change color with the acidity and alkalinity of the cell fluid. The cell fluid is acidic and reddish, and the cytosol is alkaline and blue. Anthocyanin is one of the main pigments that make up the color of petals and fruits. Anthocyanins are secondary metabolites of plants, which play an important role in physiology. Anthocyanins are pure natural anti-aging nutritional supplements. Studies have proved that they are one of the most effective plant antioxidants discovered by humans today. The bioavailability is 100%, and it can be detected in the blood 20 minut...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62C09B61/00C09B67/54
CPCC07D311/62C09B61/00C09B67/0096
Inventor 刘巍
Owner DAXINGANLING PERFECTION NOBILITY FRIGID ZONE BIO TECH CO LTD