Trichoderma harzianum solid preparation for preventing and controlling potato root rot and preparation method of preparation

A solid preparation and root rot technology, applied in the field of bioengineering, can solve the problems of unexplained biological control of Trichoderma vigor, plant disease prevention and production promotion, etc., and achieve unique formula, significant effect, and improved control effect Effect

Pending Publication Date: 2020-01-17
INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Patent CN200810154241.6 discloses a production process of using Chinese herbal medicine dregs to ferment biocontrol Trichoderma, using traditional Chinese medicine dregs in the pharmaceutical process of traditional Chinese medicine factory as a part of the culture medium to ferment and produce

Method used

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  • Trichoderma harzianum solid preparation for preventing and controlling potato root rot and preparation method of preparation

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Embodiment 1

[0043] The present embodiment provides a kind of preparation method of the Trichoderma harzianum solid preparation for preventing and controlling potato root rot, comprising the following steps:

[0044] Step 1, strain activation: inoculate the M-17 strain of Trichoderma harzianum on the slant of the PDA medium, and culture it at 25°C for 7 days in the dark;

[0045] Step 2, seed liquid culture: Inoculate the bacteria activated in step 1 into the sterilized seed medium for cultivation, the seed medium is 15g / L of sucrose, 3g / L of yeast powder, FeSO 4 ·7H 2 O 0.1g / L, MgSO 4 ·7H 2 O 1.2g / L, KH 2 PO 4 3.5g / L, NaNO 3 6.5g / L; the medium is sterilized by moist heat at 121°C for 25 minutes; the inoculum size is ; the culture condition is 3 days at 28°C on a shaker in the dark, and the shaker speed is 180rpm;

[0046] Step 3, solid fermentation: inoculate the seed liquid cultivated in step 2 into the sterilized solid fermentation medium, the components of the solid fermentatio...

Embodiment 2

[0054] The present embodiment provides a kind of preparation method of the Trichoderma harzianum solid preparation for preventing and controlling potato root rot, comprising the following steps:

[0055] Step 1. Activation of strains: inoculate the M-17 strain of Trichoderma harzianum on the slant of the PDA medium, and culture at 26°C for 7 days in the dark;

[0056] Step 2, seed liquid culture: Inoculate the bacteria activated in step 1 into the sterilized seed medium for cultivation, the seed medium is 18g / L of sucrose, 4g / L of yeast powder, FeSO 4 ·7H 2 O 0.2g / L, MgSO 4 ·7H 2 O 1.3g / L, KH 2 PO 4 3.8g / L, NaNO 3 6.7g / L; the medium is sterilized by moist heat at 121°C for 28 minutes; the inoculum size is ; the culture condition is 4 days at 27°C on a shaker in the dark, and the shaker speed is 180rpm;

[0057] Step 3, solid fermentation: inoculate the seed liquid cultivated in step 2 into the sterilized solid fermentation medium, the components of the solid fermentati...

Embodiment 3

[0065] The present embodiment provides a kind of preparation method of the Trichoderma harzianum solid preparation for preventing and controlling potato root rot, comprising the following steps:

[0066] Step 1. Activation of strains: inoculate the M-17 strain of Trichoderma harzianum on the slant of the PDA medium, and culture at 27°C for 6 days in the dark;

[0067] Step 2, seed liquid culture: Inoculate the bacteria activated in step 1 into the sterilized seed medium for cultivation, the seed medium is 20g / L of sucrose, 5g / L of yeast powder, FeSO 4 ·7H 2 O 0.2g / L, MgSO 4 ·7H 2 O 1.4g / L, KH 2 PO 4 4.0g / L, NaNO 3 6.8g / L; the medium is sterilized by moist heat at 121°C for 28 minutes; the inoculum size is ; the culture condition is 5 days at 26°C on a shaker in the dark, and the shaker speed is 200rpm;

[0068] Step 3, solid fermentation: inoculate the seed liquid cultivated in step 2 into the sterilized solid fermentation medium, the components of the solid fermentati...

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Abstract

The invention relates to a trichoderma harzianum solid preparation for preventing and controlling potato root rot and a preparation method of the preparation. The method comprises the following steps:performing activation on a fungus species, performing culture in a seed liquid, performing solid fermentation, performing spore separation, crushing a solid culture medium, and preparing the preparation to obtain the trichoderma harzianum granules or wettable powder. According to the method, traditional Chinese medicine residues are added into the solid fermentation culture medium, after fermentation is completed, the fermentation product is dried and separated to obtain trichoderma harzianum spore powder, the fermentation culture medium is dried and crushed, a carrier and spore powder are added to prepare the solid preparation, the solid preparation has a very good inhibitory effect on pathogenic bacteria of the potato root rot, such as fusarium equiseti, fusarium sambucinum, fusarium oxysporum, fusarium solani and fusarium acuminatum, and can be applied to the prevention and control process of the potato root rot, thereby reducing and preventing and controlling the occurrence of thepotato root rot, and improving the yield and quality of potatoes.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a solid preparation of Trichoderma harzianum for preventing and controlling potato root rot and a preparation method thereof. Background technique [0002] As a biocontrol fungus, Trichoderma harzianum can be used to prevent plant diseases caused by pathogens such as Pythium, Rhizoctonia solani, Fusarium, Rhizopus niger, Cylindrosporium, Sclerotinia, and Sclerotinia. Among them, root rot, dry rot, and fusarium wilt caused by Fusarium infection of potatoes are worldwide soil-borne fungal diseases. Potato Fusarium root rot has caused serious damage to potato production in recent years. Generally, the affected plots have reduced production by 13% to 35%, and serious slices of them have died or even ceased production. The control of potato root rot is generally treated with chemical fungicides. There are often residual problems when using chemical agents. At the same time, ...

Claims

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Application Information

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IPC IPC(8): A01N25/14A01N25/12A01N63/38C12N1/14A01P3/00C12R1/885
CPCA01N25/12A01N25/14C12N1/14
Inventor 王喜刚郭成瑾沈瑞清焦杨张丽荣杨波
Owner INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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