Method for removing antibiotic resistance genes in effluent of sewage plant by using ginkgo leaf modified bimetal
A technology of antibiotic resistance and ginkgo biloba, which is applied in water/sewage treatment, chemical instruments and methods, reduced water/sewage treatment, etc., can solve the problem that the ability to reduce genotype pollutants has not yet been studied, and achieve the prevention of secondary pollution. Pollution, simple process operation, and far-reaching application prospects
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Embodiment 1
[0041]At 25°C, mix 200mL of secondary effluent with 1.12g / L particles (including Ginkgo biloba modified iron-cobalt bimetallic particles, and the cobalt load in the bimetallic particles is 10%) in a 250mL conical flask with a stopper, and place in Stir on a magnetic stirrer at a speed of 250 rpm. After a specific reaction time, separate and filter, and vacuum filter the entire system water phase through a polyethersulfone water filter membrane (pore size 0.25 μm, diameter 50 mm) to complete the removal of antibiotic resistance genes in the secondary effluent.
[0042] In order to verify the effect of ginkgo leaf-modified iron-cobalt bimetallic particles on removing antibiotic resistance genes in secondary effluent water, three control examples were set up. / nano-iron-cobalt particles / nano-zero-valent iron particles modified by Ginkgo biloba / Ginkgo biloba-modified iron-cobalt bimetallic particles (cobalt loading is 10%) are mixed in a 250mL Erlenmeyer flask with a stopper and p...
Embodiment 2
[0045] Ginkgo biloba extract and 20mL ferrous sulfate solution (0.20M) were mixed in a 200mL Erlenmeyer flask with a stopper at a volume ratio of 1.5:1. After the mixture was shaken (250rpm, 25°C, 5min), 20mL of boron was added dropwise. Potassium hydride solution (0.60M) generates nanometer zero-valent iron particles modified by ginkgo leaves. In order to synthesize Ginkgo leaf modified Fe-Co bimetallic particles with different cobalt loads, 20 mL of cobalt chloride solution with concentrations of 0, 0.0019, 0.0038, 0.0095, and 0.0190 M were mixed with Ginkgo leaf modified nano-sized zero-valent iron particles , the mixed system was shaken in a constant temperature water bath shaker (250rpm, 25°C) for 20min.
[0046] At 25°C, mix 200mL of secondary sedimentation tank effluent with 1.12g / L cobalt loadings of 0%, 1%, 2%, 5%, and 10% ginkgo leaf-modified iron-cobalt bimetallic particles in a 250mL plug The Erlenmeyer flask was placed on a magnetic stirrer and stirred at a speed...
Embodiment 3
[0049] At 25°C, 200mL of secondary effluent with initial pH values of 5.00, 7.33 (original) and 9.00 were mixed with 1.12g / L cobalt loading of 10% Ginkgo biloba modified iron-cobalt bimetallic particles in a 250mL stopper cone shaped bottle, placed on a magnetic stirrer with a stirring speed of 250rpm. After a specific reaction time, separate and filter, and vacuum filter the entire system water phase through a polyethersulfone water filter membrane (pore size 0.25 μm, diameter 50 mm) to complete the removal of antibiotic resistance genes in the secondary effluent.
[0050] The removal of antibiotic resistance genes by modified iron-cobalt bimetallic particles of Ginkgo biloba at different initial pH values image 3 shown. The initial abundances of five antibiotic resistance genes (acrA-02, erm B, mef A, qnr A and tet M) in raw water were 3.59×10 4 , 9.93×10 5 , 1.12×10 4 , 9.49×10 5 and 7.76×10 5 copies / mL, the abundance of integron int I3 is 2.33×10 4 copies / mL, the...
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