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A kind of pantoea agglomerans bacterial strain and its application

A technology of Pantoea agglomerans and Pantoea agglomerans, applied in the direction of application, bacteria, fungicides, etc., to achieve a broad-spectrum effect of prevention and control

Active Publication Date: 2021-02-19
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there are no reports on the use of Pantoea agglomerans as biocontrol bacteria to simultaneously control plant pathogenic fungal diseases and mycotoxins such as scab, gray mold, rice blast, rice sheath blight, soybean anthracnose, etc.

Method used

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  • A kind of pantoea agglomerans bacterial strain and its application
  • A kind of pantoea agglomerans bacterial strain and its application
  • A kind of pantoea agglomerans bacterial strain and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Preparation of the biocontrol agent containing ZJU23 strain

[0033] 1. Acquisition of ZJU23 strain

[0034] Pantoea agglomerans ZJU23, Gram-negative, no spores, unipolar flagella, able to move. After culturing on LB medium for 24h, 1.2mm colonies can be formed, and the colonies can produce yellow pigment, round, convex surface, smooth, viscous, easy to pick up, with neat edges (see figure 1 A). Its 16S rDNA sequence is shown in the appendix, and the comparison results at NCBI show that it is Pantoea agglomerans.

[0035] The above-mentioned Pantoea agglomerans has been preserved by the General Microbiology Center of China Microorganism Culture Collection Management Committee on July 30, 2018, and the preservation number is CGMCC No.16174, so it is named Pantoea agglomerans. ZJU23 CGMCC No.16174, referred to as ZJU23 strain.

[0036] 2. Preparation of biocontrol agents

[0037] 1) The ZJU23 strain was inoculated into the LB culture solution, and at 30° C....

Embodiment 2

[0041] Example 2 Antagonistic activity of ZJU23 strain against Fusarium graminearum

[0042] 1. Determination of plate antagonistic activity of strain ZJU23 against Fusarium graminearum

[0043] Using the confrontation culture method, inoculate the Fusarium graminearum PH-1 (model bacteria) stored at 4°C on the PDA plate for activation, and use a sterilized puncher to punch evenly from the outer edge of the colony after the fungus is overgrown with the plate. A circular fungus with a diameter of 6 mm was formed. The mycelium block was inoculated in the center of the WA plate (WA medium / L: peptone 5g, glucose 10g, gravy extract 3g, sodium chloride 5g, agar 20g, pH=7.0), at about 25mm from the center around it. Inoculated with ZJU-23, the experiment took clear water as the control group. Incubate at 25°C, and record the size of the inhibition zone after the mycelium is covered with the plate.

[0044] The antibacterial activity of ZJU-23 against Fusarium graminearum was evalu...

Embodiment 3

[0065] Example 3 Control effect of ZJU23 strain on wheat scab and its DON

[0066] 1. Evaluation of the efficacy of ZJU-23 strain in the control of wheat scab in a light incubator

[0067] Collect wheat ears at flowering stage, spray and inoculate 10 8 cfu / mL (containing 0.05% Tween20) of ZJU-23 strain until the water droplets flowed (about 5 mL per ear), and then placed in a light incubator, and kept moist at 25°C for 24h. The next day, F. graminearum PH-1 spore suspension (10 5 1 / mL, containing 0.05% Tween20) was sprayed and inoculated to wheat ears (sprayed to form small water droplets, about 4 mL per ear), and then placed in a light incubator at 25°C and 90% humidity to observe the disease. Two treatment groups, ZJU23 and clear water control, were set up in the experiment. Each treatment group was inoculated with 15 wheat ears, and after inoculation, they were cultured to the time when the whole ears of the wheat ears in the clear water treatment group were onset (see ...

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Abstract

The invention discloses a Pantoea agglomerans strain and its application. The Pantoea agglomerans strain is named Pantoea agglomerans (Pantoea agglomerans) ZJU23, the preservation number is CGMCC No.16174, and the preservation date is July 30, 2018 day. The pantoea agglomerans strain obtained by the invention not only has a control effect of 50-70% on wheat scab, and reduces vomitoxin by 50-80%, but also can simultaneously prevent scab, gray mold, rice blast and soybean anthracnose Diseases caused by plant pathogenic fungi such as rice sheath blight and rice sheath blight have a wide range of prevention and control.

Description

technical field [0001] The invention relates to the technical field of development and utilization of microbial germplasm resources, in particular to a Pantoea agglomerans strain and application thereof. Background technique [0002] Wheat is the staple food for nearly half of my country's population, and ensuring the production and quality of wheat is directly related to my country's grain and food safety. In recent years, wheat scab, caused by Fusarium graminearium as the dominant species, has become one of the most important diseases affecting wheat yield in my country. Wheat scab can cause 10%-30% yield loss in typical epidemic years, and even lead to field failure in pandemic years. At the same time, when the pathogen infects wheat, it will produce a variety of mycotoxins such as Deoxynivalenol (Deoxynivalenol) and Zearalenone. Among them, DON is the mycotoxin with the highest contamination rate and contamination level in wheat and its products. The toxin has strong ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P3/00C12R1/01
CPCA01N63/00C12N1/205C12R2001/01
Inventor 陈云马忠华徐孙德尹燕妮
Owner ZHEJIANG UNIV
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