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Microbial agent used for remediation of heavy metal pollution and application thereof

A microbial agent and pollution remediation technology, applied in the direction of microorganism-based methods, microorganisms, water pollutants, etc., can solve the problems of complex process, high energy consumption, expensive equipment, etc., achieve simple preparation and use methods, and heavy metal removal rate High, easy storage and transportation effect

Pending Publication Date: 2020-01-21
SHANGHAI MARITIME UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The first two methods are relatively traditional treatment methods, which have defects such as complex process, expensive equipment, high energy consumption, and easy to cause secondary pollution, which are not conducive to large-scale promotion and application
In recent years, bioremediation technology has become a hot spot in the treatment of heavy metal pollution in the environment, but there are still problems such as low removal rate of heavy metals. At present, the method of microbial treatment of heavy metals has become a hot spot as an emerging technology

Method used

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  • Microbial agent used for remediation of heavy metal pollution and application thereof
  • Microbial agent used for remediation of heavy metal pollution and application thereof
  • Microbial agent used for remediation of heavy metal pollution and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The test soil samples (soil to be repaired) were collected from a heavy metal polluted area near a battery factory, and the heavy metal content in the surface soil in this area was higher than the third-level standard of the "Soil Environmental Quality Standard" (GB15618-2008). Repair according to the actual situation of pollution, the steps are as follows:

[0022] (1) Get back the contaminated soil sample and detect the heavy metal content therein;

[0023] (2) Prepare the microbial inoculum agent for heavy metal pollution restoration according to the following ratio of raw materials, the microbial inoculum agent is made by mixing the raw materials in the following parts by weight: 1-2 parts of water to produce Alkalibacteria, 1 -2 parts of Bacillus subtilis, 90 parts of peptone, 15 parts of yeast extract, 0.6 parts of ferric phosphate, 40 parts of urea, 1000 parts of water, and 0.5 parts of sodium hydroxide.

[0024] Mix the above-mentioned water-producing Alkaline ...

Embodiment 2

[0031] The test wastewater samples were collected from an electroplating factory, and the heavy metal content in the factory wastewater was higher than the first-level standard of the "Integrated Wastewater Discharge Standard" (GB8978-432007). Repair according to the actual situation of pollution, the steps are as follows:

[0032] (1) Sampling from the electroplating factory wastewater to detect the heavy metal content therein;

[0033] (2) The preparation method of the microbial bacterial agent used for heavy metal pollution remediation in Example 2 is the same as in Example 1.

[0034] (3) According to the microbial agent: the mass ratio of wastewater to be treated is 1:1.2, add it to the wastewater to be treated, fully aerate, mix evenly and ferment naturally, keep the temperature at 30-37°C, and ferment for 4 days. Detection of heavy metal content in remediated wastewater samples. The results are shown in Table 2:

[0035] The heavy metal content of the soil after repa...

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Abstract

The invention discloses a microbial agent used for remediation of heavy metal pollution and application thereof. The microbial agent used for remediation of heavy metal pollution comprises the following raw materials in parts by weight: 1-2 parts of Alcaligenes aquatilis, 1-2 parts of bacillus subtilis, 90-100 parts of protease, 15-20 parts of a yeast extract, 0.5-1 part of iron parts of ferric phosphate, 30-40 parts of urea, 1000 parts of water, and 0.2-0.5 part of sodium hydroxide. The microbial agent used for remediation of heavy metal pollution disclosed by the invention is prepared by performing shaking-cultivating on the raw materials at a temperature of 37 DEG C and a rotation rate of 120-130 r / min for 18 hours. Being used for remedying heavy metal pollution in soil or wastewater, the microbial agent is capable of allowing cultivation and propagation of a large number of the Alcaligenes aquatilis and the bacillus subtilis in the soil and the wastewater so as to have the urea decomposed to produce a large amount of bicarbonate radicals; and then, carbonate deposition forms by heavy metals in the soil and the wastewater under effects of the bicarbonate radicals, so that the purpose of removing heavy metals is achieved.

Description

technical field [0001] The invention relates to the technical field of environmental protection, in particular to a microbial bacterial agent for repairing heavy metal pollution and its application. Background technique [0002] The environment is the basis for the survival and development of human beings. A good ecological environment is closely related to everyone. Maintaining a good ecological environment is the key to promoting the construction of ecological civilization and maintaining ecological security. In the process of industrial development, the overall environmental conditions in some places are worrying, and heavy metal pollution is serious, among which cadmium, lead, chromium, copper, mercury, arsenic, nickel, etc. are more common heavy metal pollution. The development of industry has caused soil and water pollution to continue to increase. The content of heavy metal elements in the soil and water near some electroplating and battery factories exceeds the stand...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B09C1/10C02F101/20C12R1/05C12R1/125
CPCC12N1/20C02F3/34B09C1/10C02F2101/20
Inventor 郭娜郭章伟刘涛
Owner SHANGHAI MARITIME UNIVERSITY