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Clean production method of yeast protein feed with high content

A yeast protein and clean production technology, applied in the field of microbial fermentation technology and feed, can solve the problems of large wastewater discharge, high yeast production cost, and lack of animal protein resources, and achieve the effects of low viscosity, low cost, and simple operation

Pending Publication Date: 2020-01-24
FLSUGARPEPTIDE BIOLOGY ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the above problems of lack of animal protein resources, high yeast production cost, and large waste water discharge, and provides a clean production method for high-content yeast protein feed

Method used

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  • Clean production method of yeast protein feed with high content

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A clean production method for high-content yeast protein feed:

[0043] ①. Preparation of barley protein wet residue, dilute sugar solution, concentrated sugar solution and nutrient solution:

[0044] Grind 100kg of barley, add 200kg of water to make slurry, add liquefaction enzyme and glucoamylase to enzymatically hydrolyze, and obtain 300kg of saccharification liquid with slag;

[0045] Filter 300kg of saccharification liquid with slag, and then wash the filter residue with 50kg of clear water to obtain a total of 258kg of filtrate and 92kg of barley protein wet residue;

[0046] 253kg of dilute sugar solution was evaporated and concentrated by heating to obtain 120kg of concentrated sugar solution, and then 4kg of urea and 1kg of dipotassium hydrogen phosphate were added to completely dissolve to obtain 125kg of nutrient solution.

[0047] ② Preparation of high-density yeast fermentation broth

[0048] Sterilize the nutrient solution at 85°C for 30 minutes, then co...

Embodiment 2

[0057] A clean production method for high-content yeast protein feed:

[0058] ①. Preparation of barley protein wet residue, dilute sugar solution, concentrated sugar solution and nutrient solution:

[0059] Grind 100kg of barley, add 200kg of water to make slurry, add liquefaction enzyme and glucoamylase to enzymatically hydrolyze, and obtain 300kg of saccharification liquid with slag;

[0060] Filter 300kg of saccharification liquid with slag, and then wash the filter residue with 80kg of clear water to obtain a total of 292kg of filtrate and 88kg of barley protein wet residue;

[0061] 287kg of dilute sugar solution was evaporated and concentrated by heating to obtain 122kg of concentrated sugar solution, and then 5kg of urea and 1kg of dipotassium hydrogen phosphate were added to completely dissolve to obtain 128kg of nutrient solution.

[0062] ② Preparation of high-density yeast fermentation broth

[0063] Sterilize the nutrient solution at 85°C for 30 minutes, then co...

Embodiment 3

[0074] A clean production method for high-content yeast protein feed:

[0075] ①. Preparation of barley protein wet residue, dilute sugar solution, concentrated sugar solution and nutrient solution:

[0076] Grind 100kg of barley, add 200kg of fermentation supernatant to make slurry, add liquefaction enzyme and glucoamylase to enzymatically hydrolyze, and obtain 300kg of saccharification liquid with slag;

[0077] Filter 300kg of saccharification liquid with slag, and then wash the filter residue with 50kg of fermentation supernatant to obtain a total of 258kg of filtrate and 92kg of barley protein wet residue;

[0078] 253kg of dilute sugar solution was evaporated and concentrated by heating to obtain 120kg of concentrated sugar solution, and then 4kg of urea and 1kg of dipotassium hydrogen phosphate were added to completely dissolve to obtain 125kg of nutrient solution.

[0079] ② Preparation of high-density yeast fermentation broth

[0080] Sterilize the nutrient solution...

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Abstract

The invention relates to a clean production method of yeast protein feed with high content. The method comprises the following steps: pulverizing wheat; and adding water into the wheat and performingpulping and enzymatic hydrolysis to obtain saccharified liquid with residues, and performing solid-liquid separation to obtain wheat protein wet residues and dilute saccharified liquid; heating, evaporating and concentrating a part of dilute saccharified liquid to obtain concentrated saccharified liquid, and keeping surplus dilute saccharified liquid for later use; adding urea and dipotassium phosphate into the concentrated saccharified liquid, and performing dissolution to obtain nutrient liquid; diluting, sterilizing and cooking the dilute saccharified liquid, inoculating saccharomycetes seed liquid, introducing sterile air to culture, adding the nutrient liquid in a culturing process, stopping adding the nutrient liquid when dissolved oxygen of fermenting liquid is gradually up to 60 percent or above and performing culturing to obtain yeast fermenting liquid; and carrying out centrifugal separation to obtain high-concentration yeast pulp; mixing the high-concentration yeast pulp with the wheat protein wet residues and carrying out drying to obtain the yeast protein feed with the high content. The yeast protein feed with the high content disclosed by the invention has the advantages that must nutrients, such as aminio acid, beta-dextran, somatomedin and dietary fibers for animals are enriched, and the feed has the characteristics of promoting growth, easily digesting and enhancing immunity of the animals.

Description

technical field [0001] The invention belongs to the field of microbial fermentation technology and feed, and in particular relates to a clean production method of high-content yeast protein feed. Background technique [0002] China is a country with a large population and the largest pig raising country in the world. In 2017, the output value of China's animal husbandry industry reached 3.6 trillion yuan, 689 million pigs were slaughtered, of which 42 million sows were on hand, and pork output was 59.67 million tons. According to statistics from the Ministry of Agriculture, the national commercial feed production reached 205 million tons in 2017. Among them, 179 million tons of full-price feed, 21.51 million tons of concentrated feed, 6.9 million tons of premixed feed, including 89.74 million tons of pig feed, 90 million tons of poultry feed, 53 million tons of aquatic feed, and 8.5 million tons of cattle and sheep feed, 3.5 million tons of feed for rabbits, raccoons and p...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/18A23K10/37A23K20/132A23K20/147A23K20/163A23K20/28C12N1/14C12R1/865
CPCA23K10/12A23K10/18A23K10/37A23K20/132A23K20/147A23K20/163A23K20/28C12N1/14Y02P60/87
Inventor 符秀迪程勐万里孟龙薛芹
Owner FLSUGARPEPTIDE BIOLOGY ENG
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