Bacillus megaterium FJW1 biocontrol preparation, preparation method and application thereof

A technology of Bacillus megaterium and biocontrol agents, applied in the field of Bacillus megaterium FJW1 biocontrol preparations and its preparation, can solve problems such as environmental pollution, decline in the quality of agricultural products, and impact on human health and survival

Active Publication Date: 2020-01-31
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Agricultural production has long relied on chemical fertilizers, which not only wastes a lot of non-renewable resources, but also leads to environmental

Method used

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  • Bacillus megaterium FJW1 biocontrol preparation, preparation method and application thereof
  • Bacillus megaterium FJW1 biocontrol preparation, preparation method and application thereof
  • Bacillus megaterium FJW1 biocontrol preparation, preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Isolation and identification of Bacillus megaterium FJW1:

[0022] Put 2.5g of soil samples from Pucheng County, Weinan City, Shaanxi into 50ml of sterile water, shake at 250rpm at 30°C for 1h, then take 500μl of the soil suspension and carry out gradient dilution in a PA bottle filled with 4.5ml of sterile water. take 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 The soil suspensions of these dilutions were added to the culture medium for coating, and after 24h, 48h, 72h, and 96h of culture, the growth of plate colonies was observed, and all grown colonies were picked out for antibacterial activity experiments; Bacteria with high bacteriostatic rate and broad bacteriostatic spectrum were selected, and then further screened for saline-alkali tolerance; finally, Bacillus megaterium FJW1 was screened out.

[0023] (1) Morphological characteristics

[0024] The isolated saline-alkali-tolerant giant bacillus (Bacillus magaterium) FJW1, the colony morphology on LB solid medium is ...

Embodiment 2

[0033] Tolerance test of Bacillus megaterium FJW1:

[0034] (1) pH range test:

[0035] The seed solution of Bacillus megaterium was inoculated into LB medium with different initial pH according to the inoculum amount of 1%, and cultured at 37°C and 200r / min. After 14 hours of culture, samples were taken to measure OD 600 . The initial pH gradient of the medium was set at 3, 4, 5, 6, 7, 8, 9, 10.

[0036] LB medium: peptone 10g, yeast extract powder 5g, NaCl 10g, distilled water 1000mL, pH 7.0, sterilized by high pressure steam at 121°C for 20min.

[0037] pH 3 4 5 6 7 8 9 10 OD 600

0.043 0.052 0.139 4.361 5.764 4.415 0.19 0.016

[0038] (2) Salt tolerance test:

[0039] The seed solution of Bacillus megaterium was inoculated into LB medium with different salt concentrations according to the amount of 1%, cultured at 37°C and 200r / min, and after 14 hours of culture, samples were taken to measure OD 600 . The salt concentration gradi...

Embodiment 3

[0046] Inhibitory effect of Bacillus megaterium FJW1 on plant pathogens:

[0047]The inhibitory ability of Bacillus megaterium to pathogenic bacteria was determined by zone of inhibition method. The activated Bacillus megaterium seed solution was appropriately diluted and spread on a PDA plate, and the sterile water was used as a control group. Punch holes on the PDA plate covered with pathogenic fungi, pick the bacterial block and inoculate it upside down on the PDA plate coated with Bacillus megaterium, make the side with the fungus touch the plate, and culture the plate at 37°C for 5 days. Then measure the fungal colony diameter, calculate the bacteriostatic rate, and then analyze the bacteriostatic effect.

[0048] The plant pathogens selected in this experiment are Fusarium oxysporum F.sp.Cubense, Fusarium oxysporum, Fusarium solani, Fusarium solani, Fusarium chlamydia Fusarium chlamydosporum, Penicillium glaucum, Rhizomucor variabilis, Banana wilt fungus, Rhizopus nigr...

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Abstract

The present invention belongs to the technical field of microorganisms and discloses a bacillus megaterium FJW1 biocontrol preparation, a preparation method and an application thereof. The bacterium strain is deposited in the China Center for Type Culture Collection and has a deposit number of CCTCC NO:M2019660. The bacillus megaterium has relatively good microorganism-inhibiting effects on a variety of phytopathogens. In field experiments, tomato plants are relatively good in growth by applying the strain of the bacillus megaterium. Height and width of the plants are significantly higher thanthose of the control group inoculated with only the pathogens. Due to the fact the bacillus megaterium secretes organic acids during a fermentation process, the bacillus megaterium is difficult for producing high-content spores during fermentation. Aiming at the fact that the a high-density fermentation method aiming at the strain is provided, the method breaks through the highest level of bacillus megaterium fermentation levels and bacterium spore number in the current market, the spore number is at least 10<10> CFU/ml when tank releasing is conducted.

Description

technical field [0001] The invention belongs to the technical field of microbial bacterial agents, and in particular relates to a Bacillus megaterium FJW1 biocontrol agent and its preparation method and application. [0002] technical background [0003] Agricultural production has long relied on chemical fertilizers, which not only wastes a lot of non-renewable resources, but also leads to environmental problems such as soil quality deterioration, agricultural product quality decline, and environmental pollution, which greatly affects human health and survival. With the gradual improvement of people's awareness of environmental protection, biological control, as a non-toxic, harmless and non-polluting treatment method, has been widely used in the prevention and treatment of plant diseases. Bacillus has the advantages of rapid growth and reproduction, simple nutritional requirements, strong stress resistance, green and pollution-free, etc., and has been a research hotspot of ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/11
CPCA01N63/00C12N1/20C12N1/205C12R2001/11
Inventor 王巧梅陈振民吴鹏宇任浩然黄丽龚泽奇梁运祥
Owner HUAZHONG AGRI UNIV
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