Method for photoelectrochemically detecting aflatoxin based on homogeneous anode of bismuth tungstate

A kind of aflatoxin, photoelectrochemical technology, applied in the field of analysis and detection, analysis and detection technology, can solve the problems of complicated operation steps, decreased recognition efficiency, low sensitivity, etc., and achieve the effect of high sensitivity, simple operation and low cost

Active Publication Date: 2020-02-07
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Most of the currently reported photoelectrochemical immunosensors or aptasensors for aflatoxin detection require high-cost antibodies or labeled nucleic acid probes,

Method used

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  • Method for photoelectrochemically detecting aflatoxin based on homogeneous anode of bismuth tungstate
  • Method for photoelectrochemically detecting aflatoxin based on homogeneous anode of bismuth tungstate
  • Method for photoelectrochemically detecting aflatoxin based on homogeneous anode of bismuth tungstate

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Experimental program
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Effect test

Embodiment 1

[0027] a. Bi 2 WO 6 Preparation: Weigh 0.3g of bismuth nitrate pentahydrate and dissolve it in 20mL of dilute nitric acid with pH=1, stir magnetically until dissolved, and count it as solution A; weigh 0.17g of sodium tungstate dihydrate and dissolve it in 20mL of deionized water, Stir magnetically until dissolved, and count as solution B; add solution B dropwise to solution A, stir for 20 minutes, transfer to a 50mL autoclave, and heat at 180°C for 12 hours; after natural cooling to room temperature, anhydrous ethanol and distilled water alternately Wash and dry overnight at 60°C in a vacuum oven;

[0028] b. Preparation of Bi 2 WO 6 / ITO electrode: Weigh the pre-prepared Bi2 WO 6 The powder was added to deionized water, and ultrasonically obtained a suspension with a concentration of 1 mg / mL; drop-coated 30 μL of Bi on the surface of the pre-cleaned ITO electrode 2 WO 6 The suspension was dried at 40°C for 2 hours for later use;

[0029] c. Biorecognition and signal a...

Embodiment 2

[0032] a. Bi 2 WO 6 Preparation: Weigh 0.3g of bismuth nitrate pentahydrate and dissolve it in 20mL of dilute nitric acid with pH=1, stir magnetically until dissolved, and count it as solution A; weigh 1.49g of ammonium tungstate and dissolve it in 20mL of deionized water, stir magnetically Until it dissolves, it is counted as solution B; add solution B dropwise to solution A, stir for 20 minutes, transfer to a 50mL autoclave, and heat at 160°C for 16 hours; after naturally cooling to room temperature, wash with absolute ethanol and distilled water alternately Dry overnight at 40°C in a vacuum oven;

[0033] b. Preparation of Bi 2 WO 6 / ITO electrode: Weigh the pre-prepared Bi 2 WO 6 The powder was added to deionized water, and ultrasonically obtained a suspension with a concentration of 1 mg / mL; drop-coated 30 μL of Bi on the surface of the pre-cleaned ITO electrode 2 WO 6 The suspension was dried at 40°C for 2 hours for later use;

[0034] c. Biorecognition and signa...

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Abstract

The invention belongs to the field of analysis and detection, which relates to a method for photoelectrochemically detecting aflatoxin based on a homogeneous anode of bismuth tungstate. An ITO electrode modified by bismuth tungstate is used as a photoanode, and signal molecules (methylene blue or thionein T) can be used as electron donors to increase the photocurrent of the anode. In a homogeneoussolution, in combination with a rolling circle amplification (RCA) reaction mediated by an aflatoxin and aptamer recognition reaction and the characteristic that signal molecules are specifically embedded into a G-quadruplex, a signal enhanced detection platform is constructed. According to the present invention, the aflatoxin detection sensitivity is high, a linear range is 0.01-10000 pg/mL, andthe detection limit is as low as 2.6 fg/mL. Compared with the traditional method, the method provided by the invention has the advantages of low cost, simplicity and convenience in operation (no needof marking and electrode fixation of biomolecules), small reagent dosage and high practicability, and is expected to become one of efficient methods for detecting aflatoxin.

Description

technical field [0001] The invention relates to an analysis and detection technology, belonging to the technical field of analysis and detection. Background technique [0002] Describe the status and existing problems of the prior art closest to the present invention. [0003] Aflatoxin is mainly produced by Aspergillus flavus and Aspergillus parasiticus [Reverberi M,Ricelli A,ZjalicS,Fabbri A A,Fanelli C.Appl.Microbiol.Biotechnol.2010,87:899-911.], is the most hepatotoxic and carcinogenic and teratogenic mycotoxins [Rawal S, Kim J E, Coulombe Jr R. Res. Vet. Sci. 2010, 89:325-331.]. The International Agency for Research on Cancer (IARC) classifies aflatoxins as Group 1 human carcinogens. Among the natural pollution of grain, oil and food, aflatoxin is the most common, and the toxicity and carcinogenicity are the strongest. Among them, peanuts, peanut oil, and corn are the most serious, and their toxicity is 10 times that of potassium cyanide. Food manufacturers spend $50...

Claims

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Application Information

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IPC IPC(8): C12Q1/6804G01N27/26
CPCC12Q1/6804G01N27/26C12Q2531/125C12Q2565/607
Inventor 王光丽刘田利孙冬雪顾萌萌
Owner JIANGNAN UNIV
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