Sugar-free culture and strong seedling transplanting method for virus-free Rehmannia glutinosa seedlings

A technology of sugar-free cultivation and virus-free seedlings, which is applied in the field of plant tissue culture, can solve the problems of microbial contamination transplanting survival rate, poor growth quality of Rehmannia glutinosa seedlings, etc., and achieve good water retention performance, good air permeability, and good plant growth Effect

Active Publication Date: 2020-02-11
COTTON RES INST SHANXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of poor growth quality, serious microbial contamination and low transplanting survival rate of existing rehmannia root virus-free seedlings, the present invention provides a transplanting method for sugar-free cultivation of strong seedlings of rehmannia root virus-free seedlings

Method used

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  • Sugar-free culture and strong seedling transplanting method for virus-free Rehmannia glutinosa seedlings
  • Sugar-free culture and strong seedling transplanting method for virus-free Rehmannia glutinosa seedlings
  • Sugar-free culture and strong seedling transplanting method for virus-free Rehmannia glutinosa seedlings

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Embodiment 1

[0031] A sugar-free culture and strong seedling transplanting method of Rehmannia glutinosa virus-free seedlings comprises the following steps:

[0032] (1) Carry out the detoxification tissue culture of Rehmannia glutinosa in a test tube, multiply the detoxified seedlings, select the detoxified seedlings grown for 35 days, cut off the old roots, cut into 3cm stems, and transplant them to the sugar-free culture with vermiculite In the box, each box contains 670g vermiculite, pour 800mL nutrient solution, transplant 80 seedlings in each box, cover the box cover and seal the gap between the box cover and the box body with sealing film; the nutrient solution contains 2g / L nitric acid Calcium ammonium and 170mg / LKH 2 PO 4 , The rest is deionized water;

[0033] (2) After transplanting, put it on the culture rack in a closed room, pass the CO 2 The controller passes CO into the culture box 2 , Open the ventilation hole of the culture box, no CO for the first 4 days during the culture pe...

Embodiment 2

[0041] A sugar-free culture and strong seedling transplanting method of Rehmannia glutinosa virus-free seedlings comprises the following steps:

[0042] (1) Carry out the detoxification tissue culture of Rehmannia glutinosa in a test tube, multiply the detoxified seedlings, select the detoxified seedlings grown for 35 days, cut off the old roots, cut into 3cm stems, and transplant them to the sugar-free culture with vermiculite In the box, each box contains 670g vermiculite, pour 800mL nutrient solution, transplant 80 seedlings in each box, cover the box cover and seal the gap between the box cover and the box body with sealing film; the nutrient solution contains 2g / L nitric acid Calcium ammonium and 170mg / LKH 2 PO 4 , The rest is deionized water;

[0043] (2) After transplanting, put it on a culture rack in a closed room, and let CO into the culture box 2 , Open the ventilation hole of the culture box, no CO for the first 4 days during the culture period 2 Gas supply, start CO on...

Embodiment 3

[0049] A sugar-free culture and strong seedling transplanting method of Rehmannia glutinosa virus-free seedlings comprises the following steps:

[0050] (1) Carry out the detoxification tissue culture of Rehmannia glutinosa in a test tube, multiply the detoxified seedlings, select the detoxified seedlings grown for 35 days, cut off the old roots, cut into 3cm stems, and transplant them to the sugar-free culture with vermiculite In the box, each box contains 670g vermiculite, pour 800mL nutrient solution, transplant 80 seedlings in each box, cover the box cover and seal the gap between the box cover and the box body with sealing film; the nutrient solution contains 2g / L nitric acid Calcium ammonium and 170mg / LKH 2 PO 4 , The rest is deionized water;

[0051] (2) After transplanting, put it on a culture rack in a closed room, and let CO into the culture box 2 , Open the ventilation hole of the culture box, no CO for the first 4 days during the culture period 2 Gas supply, start CO on...

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Abstract

The invention belongs to the technical field of plant tissue culture, and particularly relates to a sugar-free culture and strong seedling transplanting method for virus-free Rehmannia glutinosa seedlings. The method includes: performing Rehmannia glutinosa virus-free tissue culture in a test tube, propagating virus-free seedlings, removing the virus-free seedlings, cutting off old roots, cuttinginto stem segments, transplanting into sugar-free culture boxes filled with vermiculite, introducing CO2 into the culture boxes, opening air exchange holes of the culture boxes, and gradually increasing the amount of the introduced CO2 along with the extension of the culture period; carrying out no illumination on the first two days in the culture period, and increasing the illumination intensityfrom the third day; after 20 days of culture in the culture boxes, opening box covers, performing seedling exercising transition under natural conditions, and moving out to a greenhouse on the fifth day. According to the invention, sterilization is not needed, direct boxing for application is performed, the transplanted tissue culture seedlings are pollution free, the transplanting survival rate is higher than 97.1%, and the plant growth vigor is good. Calcium ammonium nitrate 2g / L and monopotassium phosphate 170 mg / L are selected to replace MS (Murashige and Skoog medium) as a nutrient solution for irrigation, so that the nutrition required by the growth of tissue culture seedlings is satisfied, the procedure is simplified, and the production cost is saved.

Description

Technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a method for transplanting rehmannia glutinosa detox seedlings for sugar-free culture and strong seedlings. Background technique [0002] Rehmannia glutinosa is a commonly used medicinal material in Chinese medicine. In recent years, virus diseases in the production areas of Rehmannia glutinosa have become more and more serious, resulting in a 30-50% reduction in production or even no harvest. Rehmannia glutinosa virus disease has become a restrictive factor in the development of rehmannia glutinosa industrialization. The research on the detoxification technology of rehmannia glutinosa has been mature, but the poor growth quality of detoxification tissue culture seedlings and the low survival rate of transplantation seriously affect the industrial development of detoxification rehmannia glutinosa. [0003] In the traditional tissue culture and rapid propagation of Rehmannia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 解晓红王凌云解红娥李江辉吴宇浩王萌张鸿兴武宗信王立虎
Owner COTTON RES INST SHANXI ACAD OF AGRI SCI
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