Preparation method of biologically-fermented washing liquid derived from agallochum leaves

A technology of biological fermentation and washing liquid, which is applied in the field of preparation of biological fermentation washing liquid, can solve the problems of insufficient quality of bacterial protease, affect the washing effect, increase the production cost, etc., achieve the inhibition of bacterial spore reproduction and formation, worry-free washing, The effect of reducing emissions

Inactive Publication Date: 2020-02-21
广西美悦天香实业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, strong alkaline phosphorus-containing detergents are also easy to damage fabrics, especially pure cotton. Long-term use of these detergents that use strong alkalinity for decontamination will also burn the clothes
[0005] Although the production process of the old-style enzyme detergent uses fresh plant and kitchen waste to turn waste into treasure, sugar is still used as one of the raw materials, which increases the production cost
In addition, the production of old-style enzyme detergents did not absorb and decolorize the viscous liquid after fermentation, and fermented detergents have color
In addition, the production of old-style enzyme detergents uses natural bacteria for fermentation, and the protease of the bacteria is not high enough, which affects the washing effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Under the bushes, collect soil samples from the soil with rotten leaves, place them in a test tube filled with 5mL sterilized water, shake and mix; take 1mL of the liquid in the test tube, and dilute to 10-1, 10- 2, 10-3, 10-4, 10-5, 10-6, 10-7, take 0.2mL of soil sample liquid with 10-4, 10-5, 10-6, 10-7 dilution times and add casein respectively Plates and CMC-Na plates were coated, and then placed in a 37°C incubator for 48 hours to observe; after cultivation, there would be obvious transparent circles around the colonies on the casein plate surface, and the CMC-Na plates were stained with 0.2% Congo red for 30 minutes. Then wash off the dye solution thoroughly with distilled water and 1mol / L NaCl in turn, and a transparent circle can be seen around the colony. On the two plates, 5 colonies whose diameter of the transparent circle was larger than that of the colony were selected for continuous streak culture until pure. The purified bacterial strain is inoculated in...

Embodiment 2

[0018] Under the bushes, collect soil samples from the soil with rotten leaves, place them in a test tube filled with 5mL sterilized water, shake and mix; take 1mL of the liquid in the test tube, and dilute to 10-1, 10- 2, 10-3, 10-4, 10-5, 10-6, 10-7, take 0.2mL of soil sample liquid with 10-4, 10-5, 10-6, 10-7 dilution times and add casein respectively Plates and CMC-Na plates were coated, and then placed in a 37°C incubator for 48 hours to observe; after cultivation, there would be obvious transparent circles around the colonies on the casein plate surface, and the CMC-Na plates were stained with 0.2% Congo red for 30 minutes. Then wash off the dye solution thoroughly with distilled water and 1mol / L NaCl in turn, and a transparent circle can be seen around the colony. On the two plates, 5 colonies whose diameter of the transparent circle was larger than that of the colony were selected for continuous streak culture until pure. The purified bacterial strain is inoculated in...

Embodiment 3

[0020]Under the bushes, collect soil samples from the soil with rotten leaves, place them in a test tube filled with 5mL sterilized water, shake and mix; take 1mL of the liquid in the test tube, and dilute to 10-1, 10- 2, 10-3, 10-4, 10-5, 10-6, 10-7, take 0.2mL of soil sample liquid with 10-4, 10-5, 10-6, 10-7 dilution times and add casein respectively Spread on the plate and CMC-Na plate, and then put it into a 37°C incubator for 48 hours to observe; after culture, there will be obvious transparent circles around the colonies on the surface of the casein plate, and the CMC-Na plate will be stained with 0.2% Congo red for 30min. Then wash off the dye solution thoroughly with distilled water and 1mol / L NaCl in turn, and a transparent circle can be seen around the colony. On the two plates, 5 colonies whose diameter of the transparent circle was larger than the diameter of the colony were selected for continuous streak culture until pure. The purified bacterial strain is inocu...

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PUM

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Abstract

The invention relates to a microbial fermentation technology, in particular to a preparation method of a biologically-fermented washing liquid derived from agallochum leaves. The method comprises thefollowing steps: (1) screening of strains with high yield of cellulase and protease; (2) mixed fermentation; (3) rough filtration: filtering and centrifuging fermentation liquor to obtain clarified yellow thick liquid; (4) fine filtration: adding 4-5% of powdered activated carbon into a coarse filtrate, carrying out heating to 50 DEG C, performing sufficient stirring for 2 h, and carrying out filtering to obtain clear liquid; (5) preparation of a detergent. According to the invention, agricultural wastes are fully utilized, and the utilization rate of energy is improved; the resources of agallochum leaves and bagasse are utilized, the waste is turned into the valuable, and the very good detergent is produced, so resource utilization and environmental pollution prevention are both realized;and in addition, the biologically-fermented washing liquid has the advantages of usage of cheap raw materials, environment-friendly process, less emission and the like, and is very suitable for commercial application.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a method for preparing a biological fermentation washing solution derived from leaves of agarwood. Background technique [0002] Enzyme refers to a polymer substance with biocatalytic function. The essence of an enzyme is an enzyme. In the catalytic reaction system of an enzyme, the reactant molecule is called a substrate, and the substrate is converted into another molecule through the catalysis of the enzyme. Almost all cellular processes require the participation of enzymes to improve efficiency. Similar to other non-biological catalysts, enzymes speed up the reaction rate by reducing the activation energy of chemical reactions. Most enzymes can increase the rate of the reaction they catalyze by millions of times; It is consumed and does not affect the chemical balance of the reaction. For example, proteases can act as bioactive catalysts to catalyze the break...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11D1/00C11D3/382C11D3/386C11D3/38C11D3/04C11D3/48C11D3/60C12N1/02
CPCC11D1/00C11D3/044C11D3/381C11D3/382C11D3/38618C11D3/38645C11D3/48C12N1/02
Inventor 史学文
Owner 广西美悦天香实业有限公司
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