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Method for synchronously detecting six kinds of aflatoxins based on graphene nano material and stirring rod

An aflatoxin and nanomaterial technology, applied in the field of detection, can solve the problems affecting the detection of target substances, insufficient mechanical strength, falling off of target substances, etc., achieve good chemical inertness and thermal stability, avoid impurity chromatographic peak interference, device The effect of cost reduction

Active Publication Date: 2020-02-21
深圳茜晗健康有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The quality of the coating on the stirring rod directly determines the accuracy of the test results. Existing coatings such as sulfamethazine coating, metsulfuron-methyl coating, ractopamine coating, polydimethylsiloxane coating etc., it is easy to fall off the stirring rod together with the target substance during the analysis process, resulting in miscellaneous peaks in the mass spectrometry results, which affects the detection of the target substance and its content; and the mechanical strength is not enough, it is easy to be worn during the stirring process, resulting in adsorption on The target substance on the coating falls off during the stirring process, which affects the accuracy of the test results

Method used

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  • Method for synchronously detecting six kinds of aflatoxins based on graphene nano material and stirring rod
  • Method for synchronously detecting six kinds of aflatoxins based on graphene nano material and stirring rod
  • Method for synchronously detecting six kinds of aflatoxins based on graphene nano material and stirring rod

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preparation example Construction

[0040] The preparation method of the stir bar of the synchronous detection multiple aflatoxin based on graphene nano material of the present invention comprises the following steps:

[0041] S1 coating: ultrasonically pulverize graphene and make a graphene solution with a concentration of 0.1-0.6mg / ml, and then spray the obtained graphene solution on the stirring rod base material preheated to 120°C-130°C to obtain Graphene-coated stir bars;

[0042] S2 Conjugated Antibodies: Conjugate Aflatoxin B1 Antibody, Aflatoxin B2 Antibody, Aflatoxin G1 Antibody, Aflatoxin G2 Antibody, Aflatoxin M1 Antibody and Aflatoxin M2 Antibody to On the graphene coating, obtain the stirring bar that is coupled with aflatoxin antibody on the graphene coating.

[0043] Wherein, the stirring rod base material is any one of stainless steel, tempered glass and ceramics; in step S2, the coupling method between the graphene coating and various aflatoxin antibodies is EDC / NHS coupling Any one of couplin...

Embodiment 1

[0045] The structure of the stirring bar for synchronous detection of six aflatoxins based on graphene nanomaterials of the present invention will be described below in conjunction with specific examples. see image 3 , which is a schematic diagram of the structure of a stirring rod for simultaneous detection of six aflatoxins based on graphene nanomaterials of the present invention. The stirring rod capable of synchronous detection of six aflatoxins of the present invention is made of stainless steel, and includes a rod body 10 and a bearing surface surrounding the rod body 10 and extending radially along the rod body 10 . Preferably, the bearing surface is a helical curved surface 20 spirally extending along the axial direction of the rod body 10 , and the helical curved surface 20 is more conducive to the full contact between the stirring rod and the sample solution. The spiral curved surface 20 and the column surface 11 of the rod body 10 are coated with a graphene oxide ...

Embodiment 2

[0049] This embodiment takes Camellia japonica as an example to specifically illustrate the method for synchronous detection of six aflatoxins based on graphene nanomaterials and the stirring rod for synchronous detection of six aflatoxins based on graphene nanomaterials of the present invention. application.

[0050]Pretreatment: Camellia camellia flowers are extracted, washed, and vacuum-dried in acetonitrile-water (90:10) solvent, extracted with stirring at 50°C and concentrated to obtain Camellia camellia flower extract.

[0051] S1 adsorption: Stirring with a stirring bar to adsorb aflatoxin in Camellia japonica flower extract;

[0052] First, put 2ml of camellia flower extract into a 50ml sample bottle; then, at 50°C, stir the stir bar at ~rpm for 15 minutes to make the various yellow koji in the camellia flower extract Aflatoxin and graphene oxide nanocoating and aflatoxin B1 antibody, aflatoxin B2 antibody, aflatoxin G1 antibody, aflatoxin G2 antibody, and aflatoxin c...

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Abstract

The invention relates to a method for synchronously detecting six kinds of aflatoxins based on a graphene nanotechnology. The method comprises the following steps that: a sample solution is made to contact with a graphene coating, so that the graphene coating adsorbs aflatoxins in the sample solution; and the aflatoxins adsorbed and combined to the graphene coating are desorbed, so that the aflatoxins are released and detected. According to the method for synchronously detecting six kinds of aflatoxins, on the basis of weak pi-pi interaction formed between rich large pi bonds of the graphene nano material and the aflatoxins with aromatic ring large pi bond structures, and the characteristics of the super-large specific surface area and the like of the graphene nano material, the adsorptionand combination of the six kinds of aflatoxin in the sample liquid by the graphene coating are realized; the graphene has good chemical inertness and thermal stability, so that compared with a coating formed by other substances, the graphene does not release substances of the coating in an analysis process which are mixed with the aflatoxins, and therefore, the detection accuracy of the aflatoxins is improved.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a method for synchronous detection of six aflatoxins based on graphene nanomaterials and a stirring rod. Background technique [0002] Aflatoxin is a class of compounds with strong biological toxicity, which is metabolized by fungi such as Aspergillus flavus and Aspergillus parasiticus. Studies have shown that aflatoxins have six different structures, namely B1, B2, G1, G2, M1 and M2, and these six aflatoxins all contain benzene rings and furan rings, such as figure 1 shown. Aflatoxin is extremely harmful to the human body, mainly manifested in the following three aspects: first, it damages various tissues and organs, mainly the liver; second, it can induce a variety of cancers, such as gastric cancer, kidney cancer, Rectal cancer, breast cancer, and induce tumor growth in ovary and small intestine; third, suppress the activity of the immune system. [0003] Due to the heat ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/08
CPCG01N30/02G01N30/06G01N30/08G01N2030/062
Inventor 程金生
Owner 深圳茜晗健康有限公司
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