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A method for simultaneous detection of six kinds of aflatoxins based on graphene nanomaterials and stirring bar

A technology of aflatoxin and nanomaterials, applied in the field of detection, can solve the problems of affecting the detection of target substances, insufficient mechanical strength, and falling off of target substances, achieve good chemical inertness and thermal stability, avoid interference of chromatographic peaks of impurities, and device The effect of cost reduction

Active Publication Date: 2022-07-22
深圳茜晗健康有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The quality of the coating on the stirring rod directly determines the accuracy of the test results. Existing coatings such as sulfamethazine coating, metsulfuron-methyl coating, ractopamine coating, polydimethylsiloxane coating etc., it is easy to fall off the stirring rod together with the target substance during the analysis process, resulting in miscellaneous peaks in the mass spectrometry results, which affects the detection of the target substance and its content; and the mechanical strength is not enough, it is easy to be worn during the stirring process, resulting in adsorption on The target substance on the coating falls off during the stirring process, which affects the accuracy of the test results

Method used

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  • A method for simultaneous detection of six kinds of aflatoxins based on graphene nanomaterials and stirring bar
  • A method for simultaneous detection of six kinds of aflatoxins based on graphene nanomaterials and stirring bar
  • A method for simultaneous detection of six kinds of aflatoxins based on graphene nanomaterials and stirring bar

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preparation example Construction

[0040] The preparation method of the stirring rod for synchronous detection of multiple aflatoxins based on graphene nanomaterials of the present invention comprises the following steps:

[0041] S1 coating: ultrasonically pulverize graphene and prepare a graphene solution with a concentration of 0.1-0.6 mg / ml, and then spray the obtained graphene solution on the stirring bar base material preheated to 120 ℃-130 ℃ to obtain Stir bar coated with graphene coating;

[0042] S2 Conjugated Antibodies: Conjugate aflatoxin B1 antibody, aflatoxin B2 antibody, aflatoxin G1 antibody, aflatoxin G2 antibody, aflatoxin M1 antibody and aflatoxin M2 antibody to On the graphene coating, a stirring bar coupled with aflatoxin antibody on the graphene coating is obtained.

[0043] Wherein, the stirring rod base material is any one of stainless steel, tempered glass and ceramics; in step S2, the coupling method between the graphene coating and various aflatoxin antibodies is EDC / NHS coupling An...

Embodiment 1

[0045] The structure of the stirring rod for simultaneous detection of six kinds of aflatoxins based on graphene nanomaterials of the present invention will be described below in conjunction with specific embodiments. see image 3 , which is a schematic structural diagram of a stirring rod for synchronously detecting six kinds of aflatoxins based on graphene nanomaterials of the present invention. The stirring rod capable of synchronously detecting six kinds of aflatoxins of the present invention is made of stainless steel, and includes a rod body 10 and a bearing surface surrounding the rod body 10 and extending along the radial direction of the rod body 10 . Preferably, the bearing surface is a helical curved surface 20 spirally extending along the axial direction of the rod body 10 , and the helical curved surface 20 is more conducive to full contact between the stirring rod and the sample solution. The helical curved surface 20 and the cylindrical surface 11 of the rod bo...

Embodiment 2

[0049] The present embodiment specifically describes the method for synchronously detecting six kinds of aflatoxins based on graphene nanomaterials and the stirring rod for synchronously detecting six kinds of aflatoxins based on graphene nanomaterials by taking golden flower tea as an example of the present invention. application.

[0050]Pretreatment: Camellia japonica flowers were selected, washed and vacuum-dried in acetonitrile-water (90:10) solvent, stirred and extracted at 50°C and concentrated to obtain Camellia japonica flower extract.

[0051] S1 adsorption: stirring with a stirring rod to adsorb aflatoxin in the extract of Camellia sinensis;

[0052] First, put 2ml of Camellia japonica flower extract in a 50ml sample bottle; then at 50°C, stir the Camellia japonica flower extract with a stirring rod at ~rpm for 15 minutes, so that a variety of yellow koji in the Camellia japonica flower extract are made. Mycin and graphene oxide nanocoatings and aflatoxin B1 antibo...

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Abstract

The invention relates to a method for synchronously detecting six kinds of aflatoxins based on graphene nanotechnology, which comprises the following steps: contacting a sample solution with a graphene coating, so that the graphene coating adsorbs the aflatoxin in the sample solution Aflatoxin; the aflatoxin bound to the graphene coating is analyzed and adsorbed, so that the aflatoxin is released and detected. Compared with the prior art, the method for synchronously detecting six kinds of aflatoxins of the present invention utilizes the weak bonds formed between the abundant large π bonds of graphene nanomaterials and the aflatoxins that also have an aromatic ring large π bond structure. π-π interaction, combined with the characteristics of graphene nanomaterials such as large specific surface, realize the adsorption and binding of six kinds of aflatoxins in the sample liquid by graphene coating; in addition, graphene has good chemical inertness and thermal stability. , compared with the coating formed by other substances, it will not release the substance of the coating itself into the aflatoxin during the analysis process, thereby improving the accuracy of the aflatoxin detection.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a method for synchronously detecting six kinds of aflatoxins based on graphene nanomaterials and a stirring bar. Background technique [0002] Aflatoxin is a class of compounds with strong biological toxicity, which are metabolized by Aspergillus flavus and Aspergillus parasiticus. Studies have shown that aflatoxin has six different structures, namely B1, B2, G1, G2, M1 and M2, and the six aflatoxins all contain benzene and furan rings, such as figure 1 shown. Aflatoxin is extremely harmful to the human body, mainly in the following three aspects: first, it damages various tissues and organs, especially the liver; second, it can induce a variety of cancers, such as gastric cancer, kidney cancer, Rectal cancer, breast cancer, and induce the growth of tumors in the ovary and small intestine; third, inhibit the activity of the immune system. [0003] Due to the heat resistance ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/08
CPCG01N30/02G01N30/06G01N30/08G01N2030/062
Inventor 程金生
Owner 深圳茜晗健康有限公司
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