Unlock instant, AI-driven research and patent intelligence for your innovation.

A method for determining main sweeteners in edible flavors and fragrances by hplc-ms/ms

A technology for food flavors and sweeteners, applied in the field of physical and chemical index detection of food additives, can solve the problems of matrix interference, low detection accuracy, complex components, etc., and achieve the effects of high sensitivity, good repeatability, and strong anti-matrix interference ability

Active Publication Date: 2022-07-01
CHINA TOBACCO JIANGSU INDAL
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as a food additive, flavors have complex components and serious matrix interference, resulting in low detection accuracy

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for determining main sweeteners in edible flavors and fragrances by hplc-ms/ms
  • A method for determining main sweeteners in edible flavors and fragrances by hplc-ms/ms
  • A method for determining main sweeteners in edible flavors and fragrances by hplc-ms/ms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1. Instruments and reagents

[0043] Xylitol, acesulfame potassium, sodium saccharin, sodium cyclamate, sucralose, aspartame, neohesperidin, neotame

[0044] Standard samples of 9 sweeteners such as stevioside and stevia; methanol, ethanol, ammonium acetate (all of which are chromatographically pure); meet the requirements of first-class water in GB / T6682.

[0045] 6460 HPLC-MS / MS mass spectrometer (Agilent, USA), TM-1 vortex mixer (Wuxi

[0046]Woxin Instrument Co., Ltd.); KQ-500DE ultrasonic generator (Kunshan Ultrasonic Instrument Co., Ltd.); electronic balance (METTLER TOLEDO company, with a sense of 0.1 mg); 50 mL centrifuge tube (corning company, PP material).

[0047] 2. Preparation of matrix solvent

[0048] Choose one that does not contain xylitol, acesulfame potassium, sodium saccharin, sodium cyclamate, sucralose, aspartame,

[0049] For the edible flavors of 9 sweeteners such as neohesperidin, neotame and stevia, accurately weigh 0.5201 g of the sample, a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The application discloses a method for the determination of main sweeteners in edible flavors and fragrances by HPLC-MS / MS. The method includes matrix solvent preparation, series standard working solution preparation, sample solution preparation, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS) analysis, establishment of standard working curve and calculation of sample results. For the first time, this method uses a matrix solvent-based calibration curve for the simultaneous determination of xylitol, acesulfame potassium, sodium saccharin, sodium cyclamate, sucralose, aspartame, and new orange peel in food flavors by HPLC-MS / MS chromatograph. 9 main sweeteners including glycosides, neotame and stevia. The method prepares a suitable matrix solvent for configuring the standard working curve, eliminates the influence of the matrix effect on the quantification of the target substance in the mass spectrometry ionization process, can simultaneously detect the content of the main sweeteners in the food essence, and has the advantages of accurate results and interference. It has the advantages of less, high sensitivity and good repeatability.

Description

technical field [0001] The invention belongs to the technical field of physical and chemical index detection of food additives, and particularly relates to a method for determining main sweeteners in edible flavors and fragrances by HPLC-MS / MS. Background technique [0002] Flavors and spices are often used in the processing of food and related products. With the attention of the society to food safety issues, relevant government departments have established reasonable and effective supervision and management and safety evaluation regulations to regulate the use of flavors, spices and related additives. Production, operation and use. Sweeteners are an important class of food additives to improve the taste and flavor of food. Among them, non-sugar sweeteners have become substitutes for sucrose due to their high sweetness, low calorie, and no involvement in metabolic processes. widely used in industry. In recent years, with the improvement of consumption and awareness level,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/86
CPCG01N30/02G01N30/06G01N30/14G01N30/86G01N2030/062G01N2030/146
Inventor 廖惠云王瑞吴洋张华曹毅朱龙杰毛淑蕊沈晓晨朱怀远
Owner CHINA TOBACCO JIANGSU INDAL