In-vitro evaluation model and method for hemostatic performance of toothpaste containing bletilla striata extract

A technology for evaluating models and extracts, applied in the preparation of test samples, measurement of color/spectral characteristics, measurement devices, etc., can solve the problems that the content has not been compared and analyzed in detail, so as to reduce the cost of in vivo tests, reduce and use , The effect of improving the efficiency of research work

Pending Publication Date: 2020-02-28
SHANGHAI OCEAN UNIV
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  • Description
  • Claims
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Problems solved by technology

However, in the prior art, it is only briefly stated that Bletilla striata toothpaste has the effect of preventing oral bleeding, and no specific comparative analysis h

Method used

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  • In-vitro evaluation model and method for hemostatic performance of toothpaste containing bletilla striata extract
  • In-vitro evaluation model and method for hemostatic performance of toothpaste containing bletilla striata extract
  • In-vitro evaluation model and method for hemostatic performance of toothpaste containing bletilla striata extract

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 toothpaste hemostatic performance evaluation model in vitro

[0038] To construct an in vitro evaluation model for the hemostatic performance of toothpaste containing Bletilla striata extract, the specific method is as follows:

[0039] Collect arterial blood from female New Zealand white rabbits, centrifuge at 900rpm for 8min at 22°C, slowly transfer the supernatant liquid after centrifugation to obtain platelet-rich plasma; centrifuge the remaining part at 3000rpm for 10min, take the supernatant, and adjust the platelet density to 1×10 12 / L, to obtain platelet-poor plasma, put it in a 96-well plate, and detect the absorbance OD of the blank 0 , add different samples A to F groups (5uL / well), shake slightly. Then incubate the 96-well plate containing the samples at a constant temperature of 37°C for 5 minutes to fully react. Then add 5uL of ADP solution and shake slightly to induce platelet aggregation. After platelet aggregation occurs, the turbidity in t...

Embodiment 2

[0049] Embodiment 2 Animal experiments

[0050] Set toothpaste sample groups, respectively: A-Bletilla striata Ⅰ toothpaste group; B-Bletilla striata Ⅱ toothpaste group; C-negative toothpaste group; D-tranexamic acid toothpaste group; E-Bletilla striata extract group; F-control group ; G-blank group. IgA: F=311.7, a P<0.01, establish an animal gingival inflammation model, and verify the hemostatic performance evaluation model of toothpaste in vitro by observing the bleeding index (BOP) of the X-ray tissue and the content of immunoglobulins (IgA, IgM, IgG) in saliva instead of in vivo Feasibility of animal experiments.

[0051] IgA is secretory immunoglobulin A, which is the first immune defense line of the body against pathogens, effectively preventing harmful substances in the oral cavity from entering epithelial cells; IgM is immunoglobulin M, which is the earliest antibody in the immune response. Its content is used to evaluate the repairing effect of gingivitis in the o...

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Abstract

The invention discloses an in-vitro evaluation model and an in-vitro evaluation method for hemostatic performance of toothpaste containing a bletilla striata extract. Evaluation indexes of the in-vitro evaluation model comprise at least one of platelet aggregation rate, cyclic adenosine monophosphate (cAMP) and thromboxane B2 (TXB2) content. The in-vitro evaluation method comprises the steps of: acquiring arterial blood of an animal, centrifuging the arterial blood, adjusting plasma concentration, and detecting absorbance OD0 when the arterial blood is blank; respectively adding a toothpaste sample containing bletilla striata extracts with different concentrations and a control sample into a pore plate filled with blood plasma, slightly oscillating the samples, incubating the samples at aconstant temperature of 37 DEG C for full reaction, and adding an adenosine diphosphate solution into the samples to induce platelet aggregation reaction; and testing plasma absorbance ODt and contentof cAMP and TXB2 when the turbidity in the reaction system changes, and calculating a platelet aggregation rate for evaluating the hemostatic performance of the toothpaste. The in-vitro evaluation model and the in-vitro evaluation method effectively reduce and avoid the usage of experimental animals, can greatly improve the working efficiency of toothpaste formula research, and reduce the in-vivotest cost.

Description

technical field [0001] The invention relates to the technical field of performance testing of oral care products, relates to evaluating the hemostatic performance of toothpaste containing bletilla striata extract, and in particular to constructing an in vitro evaluation model for the hemostatic performance of toothpaste containing bletilla striata extract, which is used to evaluate the hemostatic performance of the toothpaste. Background technique [0002] Nowadays, people's oral problems such as dental caries, bad breath, gingivitis, periodontitis and tooth sensitivity are becoming more and more prominent, and the quality and function requirements of oral care products are also getting higher and higher. However, it is still difficult to verify the anti-inflammatory and repair functions of oral care raw materials and products. The traditional testing method is to verify through animal experiments. In March 2009, EU Cosmetics Regulation (EC) 1223 / 2009 was officially implemen...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N1/28
CPCG01N21/3103G01N1/28
Inventor 吴文惠郭庆冯婧文包斌郭锐华
Owner SHANGHAI OCEAN UNIV
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