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A strain of Lactobacillus johnsonii and its application

A technology of Lactobacillus johnsonii and microbial strains, applied in the field of Lactobacillus johnsonii, can solve problems such as human health and environmental hazards

Active Publication Date: 2021-04-20
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although antibiotics play a certain positive role in disease prevention and resistance, their long-term use brings harm to human health and the environment is self-evident

Method used

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  • A strain of Lactobacillus johnsonii and its application
  • A strain of Lactobacillus johnsonii and its application
  • A strain of Lactobacillus johnsonii and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Isolation, screening and identification of embodiment one lactic acid bacteria

[0027] 1.1 Isolation of lactic acid bacteria

[0028] 50 fresh feces of healthy pigs from several pig farms in Qinghai Province were collected. Weigh 10g of feces sample and mix with 90mL sterile PBS and filter, take 1mL of the filtrate for gradient dilution, and dilute 10 -3 、10 -4 、10 -5 The dilutions were spread on MRS plates containing 0.75% calcium carbonate, and cultured under anaerobic conditions at 37°C for 24h. In each sample, about 4 to 7 single colonies that produced calcium-dissolving circles with different shapes and colors were streaked and purified on the MRS plate for three times, and the single colonies were picked and inoculated in MRS liquid medium, and cultured anaerobically at 37°C. After 24 hours, 325 isolates were initially isolated.

[0029] The MRS liquid culture medium formula of the above-mentioned use of the present invention is as follows:

[0030] Peptone...

Embodiment 2

[0055] Example 2 Study on the in vitro probiotic properties of Lactobacillus johnsonii GHZ10a

[0056] 2.1 Preparation of Lactobacillus johnsonii GHZ10a seed solution

[0057] Pick a single colony from the purified MRS plate and inoculate it in 10 mL of MRS medium, culture at a constant temperature of 37 °C for 24 hours, pass two times in a row to prepare the isolated bacterial seed liquid, and set aside.

[0058] 2.2 Determination of growth curve and pH curve of Lactobacillus johnsonii GHZ10a

[0059] Take the Lactobacillus johnsonii GHZ10a seed solution in 2.1 and inoculate it in the MRS medium at 2%, and culture it statically at 37°C. Take the uninoculated liquid medium as the control, within 0-24h after inoculation and culture, at intervals of 2h Take a sample once to determine the OD of the bacterial solution (600nm) value and pH value. the result shows( Figure 7 ), Lactobacillus johnsonii GHZ10a entered logarithmic growth phase 4h after inoculation, OD within 4-8h ...

Embodiment 3

[0088] The safety research of embodiment three Lactobacillus johnsonii GHZ10a

[0089] 3.1 Drug susceptibility test (K-B method) of bacterial strain GHZ10a

[0090] Centrifuge the activated strain GHZ10a and adjust the concentration with sterile PBS to about 1×10 6 CFU / mL, use a sterilized cotton swab to dip the bacterial solution and spread it evenly on the MRS solid plate; use sterile tweezers to evenly place the drug-sensitive sheet on the surface of the plate, and after anaerobic incubation at 37°C for 24 hours, record the results of each drug-sensitive sheet The diameter of the antibacterial zone of the tablet was repeated 3 times to obtain the average value. In this test, Escherichia coli (Escherichia coli) ATCC 25922 was selected as the quality control strain, and the minimum inhibition zone diameter for antimicrobial susceptibility in the American CLSI (Clinical and Laboratory Standards Institute) international standard was used to determine the strain's resistance to...

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Abstract

The invention discloses a strain of Lactobacillus johnsonii and its application in diarrhea of ​​piglets, lambs and calves. The strain is isolated from healthy pig feces, and has good in vitro probiotic potential and safety. When the Lactobacillus johnsonii of the present invention is combined with other bacterial strains and applied to young animals with diarrhea, the bacterial strains have obvious preventive and therapeutic effects on diarrhea cases of different degrees.

Description

technical field [0001] The invention relates to a strain of Lactobacillus johnsonii and its application. Background technique [0002] my country is a large animal husbandry country, and the healthy development of livestock and poultry is the key to the economic development of the breeding industry. In the pig industry, piglet diarrhea is the most serious problem faced by large-scale farms, and has become one of the main problems affecting the healthy and stable development and economic benefits of the pig industry. Many pathogenic microorganisms can cause diarrhea in young animals such as piglets, lambs and calves, among which Escherichia coli, Salmonella and streptococcus are the main ones. In large-scale farms, the solution to such problems is the use of antibiotics. Although antibiotics play a certain positive role in disease prevention and resistance, their long-term use brings harm to human health and the environment is self-evident. With the restriction and prohibi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18A23K50/10A23K50/30A61K35/747A61P1/12C12R1/225
CPCA23K10/18A23K50/30A23K50/10A61K35/747A61P1/12C12R2001/225C12N1/205A23V2400/151
Inventor 李有全贾丹刘爱红王锦明孟林明王佳慧李贺海关贵全刘军龙殷宏罗建勋石红梅赵索南任巧云杨吉飞刘志杰独军政高闪电刘光远李东平
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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