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Eurotium cristatum selenium-enriched strain and domestication and fermentation method

A technology of R. coronoides and a fermentation method, which is applied to the fields of selenium-enriched strains of R. coronoidans and the fields of domestication and fermentation, can solve the problems of low conversion rate and large body irritation, and achieves the effect of meeting the needs of industrialized production.

Inactive Publication Date: 2020-03-17
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Organisms generally take in trace elements in the form of inorganic salts, which has a low conversion rate and is highly irritating to the body

Method used

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  • Eurotium cristatum selenium-enriched strain and domestication and fermentation method
  • Eurotium cristatum selenium-enriched strain and domestication and fermentation method
  • Eurotium cristatum selenium-enriched strain and domestication and fermentation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Screening of selenium-enriched wild strains of R. coronoidans

[0026] Weigh 10g of Fuzhuan tea with bright yellow closed capsules from the commercially available "Jingwei Fucha" in Xi'an, put it in a mortar and roughly crush it, and then add 90mL of sterile water with 3-5 glass beads in the Erlenmeyer flask. Shake and mix at 150rpm for about 30min. Take 1 mL of the spore mixture to make a 10-fold gradient dilution with sterile water, and select 10 -3 and 10 -4 Two gradients of spore suspensions were drawn and spread on PDA solid medium containing 10 mg / L selenium, and cultured at 30°C for 60 h. The colonies with larger colonies and bright yellow color were picked and streaked on PDA plates containing 10mg / L selenium element to continue screening for 8-10 generations.

Embodiment 2

[0027] Example 2 Mutagenesis, domestication and isolation and purification of wild strains

[0028] The bacterial moss screened in Example 1 was eluted with sterile physiological saline, and dispersed with glass beads, and the concentration was adjusted to 2 × 10 6 -2×10 8 spore suspension of spores / mL, draw a certain amount of spore suspension and place it in a petri dish (so that the liquid level does not exceed 3mm), irradiate it with a 20W UV lamp at a distance of 30cm for 25min, and absorb 0.1mL of the bacterial suspension. PDA solid plates containing various concentrations (0; 5; 10; 15; 20; 25 mg / L) of inorganic selenium and coated. Incubate at 30°C for 60h. Select several colonies with larger colonies and bright colors on different concentrations of selenium and streak them on the PDA solid plate containing 10 mg / L inorganic selenium, compare the colony size and colony appearance again, and select the largest colony and the brightest color as the dominant bacteria. ...

Embodiment 3

[0030] Example 3 Preservation of selenium-enriched strains of R. coronoidans

[0031] Pick the selenium-enriched selenium-enriched R. coronoides fungus moss screened in Example 2 on a solid PDA slant, cultivate at 30°C for about 60 hours, add 20 mL of sterile water, and scrape the fungal moss with a bamboo stick. After mixing, the spore concentration is about 2×10 6 --2×10 8 pcs / mL. Add PDA liquid medium containing 50% glycerol at a volume ratio of 1:1 and mix well. Pre-frozen at -20°C first, and then transferred to -80°C for medium and long-term storage, the spore germination rate is above 90%.

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Abstract

The invention discloses an eurotium cristatum selenium-enriched strain Eurotium cristatum XD-01, which is preserved in the China General Microbiological Culture Collection Center on April 2, 2018, anda preservation number is CGMCC No. 15395. In addition, the invention also discloses a domestication and fermentation method of the strain. The eurotium cristatum selenium-enriched strain disclosed bythe invention is derived from Fuzhuan tea, which has the advantages of ecological safety and harmlessness, the strain can tolerate inorganic selenium on an optimized culture medium, the inorganic selenium can be efficiently converted into organic selenium, the intracellular organic selenium content is as high as 690mg / kg, and the strain can be used as a production strain for efficiently converting the inorganic selenium into the organic selenium, so that the industrial production requirement is met.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, in particular to a selenium-enriched strain of R. coronoides and a method for domestication and fermentation. Background technique [0002] Selenium is one of the essential trace elements in human and animal bodies, which can protect cell membranes and enable them to play important functions and enhance immunity. Selenium deficiency can greatly reduce the body's antioxidant capacity, and lead to a variety of diseases, such as Keshan disease, Kashin-Beck disease, heart disease and tumors. The form of selenium deficiency in my country's population is severe, and more than 20 provinces, municipalities, autonomous regions and municipalities directly under the Central Government have selenium deficiency. Organisms generally ingest trace elements in the form of inorganic salts. This method has a low conversion rate and is highly irritating to the body. [0003] The present invention sele...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/02C12N1/36C12N13/00C12R1/645
CPCC12N1/02C12N1/14C12N1/36C12N13/00C12N1/145C12R2001/645
Inventor 范代娣惠俊峰段志广唐育岐
Owner NORTHWEST UNIV
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