Fluorescent probe for detecting viscosity as well as preparation method and application thereof
A fluorescent probe and viscosity technology, applied in the field of analytical chemistry, to achieve the effect of convenient purification, high yield and high sensitivity
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Embodiment 1
[0032] Example 1 Synthesis of Fluorescent Probes
[0033] (1) Weigh 4.78 g of methyl isoflavone (29.9 mmol) and 2.40 g of malononitrile (36.2 mmol) into an eggplant-shaped bottle containing 25 mL of acetic anhydride, reflux in an oil bath at 120°C for 14 hours, and then vacuum The solvent was evaporated. Deionized water (80 mL) was added to the residue, and the mixture was refluxed for 0.5 h, then extracted twice with dichloromethane, and the organic layer was washed with anhydrous Na 2 SO 4 Drying, the system was distilled under reduced pressure to spin dry the solvent, and the purified product was obtained through column chromatography separation. The mobile phase of the column chromatography separation was dichloromethane and petroleum ether with a volume ratio of 1:5 to obtain a red solid 2-(2-methanol) -4H-chromene-4-methylene) malononitrile;
[0034]
[0035] (2) Weigh 0.208 g 2-(2-methyl-4H-chromene-4-methylene) malononitrile (0.1 mol), and 0.273 g 4-(diphenylamin...
Embodiment 2
[0040] Example 2 Emission spectra of fluorescent probes in different viscosity systems
[0041] A test mother solution of dimethyl sulfoxide (DMSO) with a concentration of 1 mM fluorescent probe TBM-V obtained in Example 1 was prepared for use. In the test solution, take 3 mL solvents of different ratios of glycerol and methanol (glycerol: methanol is 0:10, 1:9, 2:8, 3:7, 4:6, 5:5, 6:4, 7: 3, 8:2, 9:1, 10:0), then add the probe mother solution (final concentration is 10 μM), perform fluorescence scanning (excitation wavelength 510 nm, detection band 520-900 nm), and measure the Relative fluorescence intensity, such as image 3 shown. Depend on image 3 It can be seen that with the increase of solvent viscosity, the relative fluorescence intensity becomes stronger.
Embodiment 3
[0042] Example 3 Application of Fluorescent Probes to Detect Blood Viscosity
[0043] Prepare the test mother solution of dimethyl sulfoxide (DMSO) with a concentration of 1 mM for the fluorescent probe TBM-V obtained in Example 1 for use; use the mouse eyeball blood collection method to obtain normal mice (Normal mice) with small tumors mice (Tumor mice), and the blood of cancer mice treated with Paclitaxel; then the probe mother solution (final concentration 10 μM) was added to the blood of the three groups, and the fluorescence scanning was performed (excitation wavelength 510 nm, The detection band is 520-900 nm), and the relative fluorescence intensity in each system is measured, such as Figure 4 shown. Depend on Figure 4 It can be seen that the fluorescence intensity of cancer mouse blood is stronger than that of normal mouse blood, indicating that the blood viscosity in cancer mice is high; the fluorescence intensity of mouse blood after paclitaxel treatment has weake...
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