Unlock instant, AI-driven research and patent intelligence for your innovation.

Biopeptide Composition for Promoting Skin Damage Repair

A biological peptide and skin injury technology, applied in the biological field, can solve problems such as skin tissue loss, limited donor sources, immune rejection, etc., and achieve the effect of promoting wound healing, promoting skin wound healing, and promoting angiogenesis

Active Publication Date: 2021-01-08
北医博大(北京)皮肤病医学研究院有限公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Trauma, extensive burns, diabetes or other chronic diseases can cause severe loss of skin tissue. At present, autologous, allogeneic skin transplantation or artificial substitutes through tissue engineering are commonly used in clinical repair, but these methods have new donor areas. Issues such as trauma, limited donor sources, and immune rejection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Biopeptide Composition for Promoting Skin Damage Repair
  • Biopeptide Composition for Promoting Skin Damage Repair
  • Biopeptide Composition for Promoting Skin Damage Repair

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Preparation of Skin Repair Solution 1:

[0024] A biological polypeptide composition for promoting skin damage repair, its preparation method is as follows:

[0025] Mix the defatted low-value small yellow croaker leftovers with distilled water, add 3% (E / S, based on substrate weight) compound enzyme, and mix well to carry out hydrolysis.

[0026] Put 20g of low-value fish scraps (defatted) and 100mL of distilled water in a 500mL Erlenmeyer flask with a stopper, add 3% (E / S, based on substrate weight) compound enzyme, and mix well.

[0027] The composite enzyme is a composite protease composed of flavor protease:papain:bromelain in a mass ratio of 2:1:1.

[0028] The hydrolysis process is as follows: constant temperature oscillating enzymatic hydrolysis for 300 minutes, after the hydrolysis is completed, the temperature is raised to 95° C. to inactivate the enzyme, cooled to room temperature, and suction filtered.

[0029] The hydrolysis process is as follows: enzymat...

Embodiment 2

[0032] Prepare Skin Repair Solution 2:

[0033] A biological polypeptide composition for promoting skin damage repair, its preparation method is as follows:

[0034] Mix the defatted low-value small yellow croaker leftovers with distilled water, add 3% (E / S, based on substrate weight) compound enzyme, and mix well to carry out hydrolysis.

[0035] Put 20g of low-value fish scraps (defatted) and 100mL of distilled water in a 500mL Erlenmeyer flask with a stopper, add 3% (E / S, based on substrate weight) compound enzyme, and mix well.

[0036] The composite enzyme is a composite protease composed of flavor protease:papain:bromelain in a mass ratio of 2:1:1.

[0037] The hydrolysis process is as follows: constant temperature oscillating enzymatic hydrolysis for 300 minutes, after the hydrolysis is completed, the temperature is raised to 95° C. to inactivate the enzyme, cooled to room temperature, and suction filtered.

[0038] The hydrolysis process is as follows: enzymatic hydr...

Embodiment 3

[0043] Embodiment 3 tests the repairing function to skin

[0044] Wound Healing Test:

[0045] Experimental animals: Choose 50 healthy female BALB / c mice, about 8 weeks old, with a body weight of 35-40g, and divide them into 5 groups, with 10 mice in each group. There is no statistical difference between each group, and there are no mice until the end of the experiment die.

[0046] The control group used 3 groups, the one without modeling was used as the blank control group, the normal saline was used as the normal saline control group, and the aqueous solution of 0.2% xanthate glucoside A (dissolved in water after dissolving with a small amount of butanediol) was used as the xanthate eucalyptin A solution. Theoside A control group.

[0047] Group 1: blank control group (no modeling);

[0048] Group 2: normal saline group (modeling);

[0049] Group 3: 0.2% xanthate glucoside A control group (modeling);

[0050] Group 4: skin repair solution 1 intervention group (modeling...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a biological polypeptide composition for promoting skin injury repair, which has a very good effect on skin repair and can promote secretion of epidermal growth factors of a wound surface. A preparation method comprises the following steps: mixing degreased fish leftovers with distilled water, adding 3% (E / S, based on the weight of a substrate) of compound enzyme, uniformlymixing, and hydrolyzing. The traditional Chinese medicine composition can further comprise Gordonia chrysandra Cowan glycoside A.

Description

technical field [0001] The invention relates to a biological polypeptide composition for promoting skin damage repair and belongs to the field of biotechnology. Background technique [0002] Peptides are formed by decondensing the amino group of one amino acid and the carboxyl group of another amino acid and connecting them through peptide bonds. They are functional and structural fragments of proteins. It is divided according to the number of amino acids: those with the number of amino acids in the range of 2-10 are called oligopeptides or oligopeptides; those with the number of amino acids in the range of 10-50 are called polypeptides. Polypeptide is a high molecular compound between amino acid and protein, and has strong biological activity. It is reported in the prior art that the enzymatic hydrolyzate of corn protein has good anti-inflammatory effect on trinitrobenzenesulfonic acid-induced colitis in rats, and also has good antioxidant activity. [0003] Trauma, exten...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/01A61K31/7034A61P17/02
CPCA61K31/7034A61K38/012A61P17/02A61K2300/00
Inventor 朱由瑾
Owner 北医博大(北京)皮肤病医学研究院有限公司