Supercharge Your Innovation With Domain-Expert AI Agents!

Process for preparing high-purity pregnant mare serum gonadotropin (PMSG)

A technology of gonadotropin and pregnant horse serum, which is applied in the preparation method of peptides, peptides, organic chemistry, etc., can solve the problems of low potency and poor quality, and achieve the effects of high potency, low risk of harm, and high purity

Active Publication Date: 2020-04-03
NINGBO SANSHENG BIOLOGICAL TECH CO LTD +1
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the defects of low titer and poor quality in the PMSG preparation process in the prior art, the present invention provides a PMSG preparation process to obtain high-purity PMSG with a titer ≥ 1500IU / mg

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Process for preparing high-purity pregnant mare serum gonadotropin (PMSG)
  • Process for preparing high-purity pregnant mare serum gonadotropin (PMSG)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation process of pregnant horse serum gonadotropin of the present embodiment comprises the following steps:

[0026] (1) Preparation of PMSG crude product: add 1.5% metaphosphoric acid to adjust the pH of pregnant horse plasma to 3.8, stir for 10 minutes, centrifuge and filter, and the filtrate is concentrated to 1 / 2 volume, then add -20 ℃ absolute ethanol to make the ethanol concentration of the solution system 45% (v / v), after stirring for 10 minutes, centrifuge, add -20 ℃ absolute ethanol to the filtrate to make the concentration of alcohols in the solution system 83% (v / v), after stirring for 20 minutes, let stand for 12 hours to separate layers , The supernatant was discarded by siphoning, and the lower precipitate was collected and vacuum-dried for 48 hours to obtain crude PMSG.

[0027](2) Preparation of PMSG intermediate product: add 10% (m / v) sodium 4-hydroxybenzoate solution to the crude product of PMSG, the mass ratio of the crude product of PMSG an...

Embodiment 2

[0030] The preparation process of pregnant horse serum gonadotropin of the present embodiment comprises the following steps:

[0031] (1) PMSG crude product preparation: add 1.5% metaphosphoric acid to adjust the pH of pregnant horse plasma to 3.8, stir for 10 minutes, centrifuge and filter, and the filtrate is concentrated to 1 / 2 volume, then add -20 ℃ absolute ethanol to make the ethanol concentration of the solution system 45% (v / v), stirred for 10 minutes, centrifuged, added -20 ℃ absolute ethanol to the filtrate to make the concentration of alcohols in the solution system 83% (v / v), stirred for 20 minutes, allowed to stand for 12 hours to separate layers , The supernatant was discarded by siphoning, and the lower precipitate was collected and vacuum-dried for 48 hours to obtain crude PMSG.

[0032] (2) Preparation of PMSG intermediate product: add 10% (m / v) sodium 4-hydroxybenzoate solution to the crude product of PMSG, the mass ratio of crude product of PMSG and sodium 4...

Embodiment 3

[0035] The preparation process of pregnant horse serum gonadotropin of the present embodiment comprises the following steps:

[0036] (1) PMSG crude product preparation: add 1% metaphosphoric acid to adjust the pH of pregnant horse plasma to 3.5, stir for 5 minutes, centrifuge and filter, and the filtrate is concentrated to 1 / 2 volume, then add -20 ℃ absolute ethanol to make the ethanol concentration of the solution system 40% (v / v), after stirring for 20 minutes, centrifuge, add -20 ℃ absolute ethanol to the filtrate to make the concentration of alcohols in the solution system 80% (v / v), after stirring for 20 minutes, let stand for 12 hours to separate layers , The supernatant was discarded by siphoning, and the lower precipitate was collected and vacuum-dried for 48 hours to obtain crude PMSG.

[0037] (2) Preparation of PMSG intermediate product: add 10% (m / v) sodium benzoate solution to PMSG crude product, the mass ratio of PMSG crude product and sodium benzoate is 1:0.1, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a process for preparing high-purity pregnant mare serum gonadotropin (PMSG). The process comprises the following steps: (1) adjusting a pH of pregnant mare blood plasma to 3.0to 4.0, carrying out solid-liquid separation, concentrating filter liquor, adding an alcoholic substance to enable an alcoholic substance concentration of a solution system to be 30% to 55% (v / v), then, carrying out solid-liquid separation, adding an alcoholic substance into filter liquor to enable an alcoholic substance concentration of a solution system to be 70% to 90% (v / v), and drying obtained precipitates, so as to obtain crude PMSG; (2) adding a toluene derivative or ethylbenzene derivative solution into the crude PMSG, carrying out solid-liquid separation, then, adding an alcoholic substance to precipitates, and drying obtained precipitates, so as to obtain a PMSG intermediate; and (3) adding an ammonium salt and / or a calcium salt and / or a potassium salt solution and an alcoholicsubstance into the PMSG intermediate, carrying out solid-liquid separation, then, adding an ammonium salt and / or a calcium salt and / or a potassium salt solution and an alcoholic substance into clear liquid again, carrying out solid-liquid separation, then, adding an alcoholic substance into clear liquid, and drying obtained precipitates, thereby obtaining refined PMSG.

Description

technical field [0001] The invention belongs to the technical field of pregnant horse serum gonadotropin production, and in particular relates to a preparation process of high-purity pregnant horse serum gonadotropin. Background technique [0002] Pregnant horse serum gonadotropin (referred to as PMSG) is a glycoprotein gonadotropin secreted by the allantoic chorionic cells of pregnant mare. PMSG has dual activities similar to follicle-stimulating hormone (FSH) and luteinizing hormone (LH), but the effect of FSH is the main one, and it has obvious follicle-stimulating effect; meanwhile, it also has certain functions of ovulation-stimulating and corpus luteum formation, which is beneficial to male animals. It has the function of promoting the development of seminiferous tubules and the differentiation of sex cells. In practice, PMSG can regulate the simultaneous estrus of female animals, realize controllable and synchronous batch production, shorten mating time, reduce the p...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K14/59C07K1/30
CPCC07K14/59
Inventor 于钢沙乐俞通泰王荣庆
Owner NINGBO SANSHENG BIOLOGICAL TECH CO LTD
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com