Preparation method of Ag/Cu alloy nanocluster and application of Ag/Cu alloy nanocluster in Ag<+> detection

A technology of alloy nanometers and mixtures, applied in the field of fluorescent probes, can solve the problems of high cost, complex preparation method of alloy nanoclusters, and unsatisfactory application effect of detection methods, and achieves low cost, good practical application value and good optical properties. Effect

Active Publication Date: 2020-04-03
HEBEI UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a preparation method of Ag/Cu alloy nanoclusters and its + The application in detection to solve the compl...

Method used

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  • Preparation method of Ag/Cu alloy nanocluster and application of Ag/Cu alloy nanocluster in Ag&lt;+&gt; detection
  • Preparation method of Ag/Cu alloy nanocluster and application of Ag/Cu alloy nanocluster in Ag&lt;+&gt; detection
  • Preparation method of Ag/Cu alloy nanocluster and application of Ag/Cu alloy nanocluster in Ag&lt;+&gt; detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Preparation of DPA@Ag / Cu NCs fluorescent probe

[0041] Add 950 μL ultrapure water to the reagent bottle, then add DPA solution (100 mM, 800 μL), AgNO 3 solution (100 mM, 200 μL) and Cu(NO 3 ) 2 Solution (100 mM, 50 μL), mixed at room temperature, and finally placed on a magnetic stirrer while irradiating with UV light for 6 h, to prepare 2 mL of DPA@Ag / Cu NCs solution. Prepared sample DPA at room temperature @ The Ag / Cu NCs were placed for 12 h, then moved to a centrifuge tube and placed on the material tray of a freeze dryer and placed in a cold trap for 4 h to be pre-frozen. After lyophilization, the sample was taken out after 24 h of lyophilization, and the DPA@Ag / Cu NCs fluorescent probe was obtained, which was used for subsequent experiments.

[0042] DPA@Ag / Cu NCs DPA @ Characterization of Fluorescent Probes for Ag / Cu NCs

[0043] For prepared DPA @ Ag / Cu NCs fluorescent probes use scanning electron microscopy (SEM), transmission electron microscopy (TEM),...

Embodiment 2

[0045] Ag in buffer + detection

[0046] Different concentrations of Ag + (0.05, 0.1, 0.5, 1, 5, 15, 30, 50, 80, 100, 200, 500, 800, 100, 1200, 1500 µM) were added to the prepared DPA @ Ag / Cu NCs fluorescent probe solution, then add phthalic acid-hydrochloric acid buffer solution to adjust the pH of the system to 2.8, incubate at room temperature for 30 min, and collect fluorescence emission in the range of 450-650 nm at an excitation wavelength of 353 nm Spectrum, and draw the standard curve, the result is as Figure 11 shown. When Ag + concentration in the range of 0-1500µM, with the Ag + As the concentration increases, the fluorescence intensity of the system decreases gradually, and the Ag + At high concentrations, fluorescence quenching gradually slows down. Figure 11 are different concentrations of Ag + The relationship diagram of the fluorescence change caused by it, the inset is the corresponding standard curve, and the results show that [Ag + ] and Δ F It h...

Embodiment 3

[0052] Experimental condition optimization

[0053] Change the UV lamp irradiation time in the preparation method of Example 1 (other conditions are the same as Example 1), and the results show that 6 h of irradiation is the best time ( Figure 10 a).

[0054] Changing DPA and AgNO 3 The dosage ratio and Cu(NO 3 ) 2 The dosage (other conditions are the same as Example 1), the results show that [Ag + ]:[DPA]=1:4( Figure 10 b) and [Cu 2+ ]=2.5 mM( Figure 10 c), the prepared DPA @ Ag / Cu NCs fluorescent probes have strong fluorescence intensity.

[0055] The influence of changing different buffer solutions and their pH on the fluorescence change of the detection system (other conditions are the same as in Example 2), DPA @ Ag / Cu NCs solution and Ag + In Britton-Robinson buffer solution (B.R buffer solution), phthalic acid-hydrochloric acid buffer solution (KHP-HCl buffer solution), glycine-hydrochloric acid buffer solution (Gly-HCl buffer solution), disodium hydrogen p...

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Abstract

The invention discloses a preparation method of an Ag/Cu alloy nano-cluster and application of the Ag/Cu alloy nano-cluster in Ag < + > detection. The preparation method comprises the following steps:sequentially adding a D-penicillamine solution, an AgNO3 solution and a Cu (NO3) 2 solution into a container filled with ultrapure water, and uniformly mixing to obtain a mixture; and stirring the mixture with a magnetic stirrer, and irradiating with an ultraviolet lamp for 4-10 hours to obtain the DPA-Ag/Cu alloy nano-cluster solution capable of emitting yellow fluorescence. The preparation method is low in cost, easy and convenient to operate, free of toxic reagents and more environmentally friendly. The prepared DPA-coated Ag/Cu NCs are used for constructing a fluorescence sensor for detecting Ag < + > in a water environment. The fluorescence quenching value and the Ag < + > concentration of the fluorescent probe have a good linear relation within the range of 0.01-800 [mu] M, the detection selectivity and sensitivity are high, and the fluorescent probe has an important practical application value.

Description

technical field [0001] The invention relates to the technical field of fluorescent probes, in particular to a preparation method of Ag / Cu alloy nanoclusters and its + application in detection. Background technique [0002] Metal nanoclusters usually contain several to dozens of metal atoms. When the size of metal nanoparticles is further reduced to close to the Fermi wavelength of electrons, the free movement of electrons inside the particles will be very restricted, and the continuous energy level structure will become unsatisfactory. Continuous, showing some molecular-like behaviors, such as electron transfer in HOMO–LUMO orbitals, intrinsic magnetism, photoluminescence phenomena, etc. Metal nanoparticles with a size less than 2 nm are generally defined as metal nanoclusters (NCs). Compared with conventional fluorescent materials (such as organic dyes and quantum dots), metal nanoclusters have better photoelectric properties, super The advantages of small size, large spe...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6402
Inventor 董江雪安苗李慧苏明申世刚高舒王明晨
Owner HEBEI UNIVERSITY
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