A kind of preparation method of ag/cu alloy nanocluster and its application in ag + Applications in testing
A technology of alloy nanometers and mixtures, applied in the field of fluorescent probes, can solve the problems of high cost, complex preparation method of alloy nanoclusters, and unsatisfactory application effect of detection methods, and achieves low cost, good practical application value and good optical properties. Effect
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Embodiment 1
[0040] Preparation of DPA@Ag / Cu NCs fluorescent probe
[0041] Add 950 μL ultrapure water to the reagent bottle, then add DPA solution (100 mM, 800 μL), AgNO 3 solution (100 mM, 200 μL) and Cu(NO 3 ) 2 Solution (100 mM, 50 μL), mixed at room temperature, and finally placed on a magnetic stirrer while irradiating with UV light for 6 h, to prepare 2 mL of DPA@Ag / Cu NCs solution. Place the prepared sample DPA@Ag / Cu NCs at room temperature for 12 h, then move it to a centrifuge tube and put it on the material tray of a freeze dryer and put it into a cold trap for 4 h to pre-freeze. The sample was quickly placed on a freeze-drying rack for lyophilization, and after 24 h of lyophilization, the sample was taken out to obtain the DPA@Ag / Cu NCs fluorescent probe for subsequent experiments.
[0042] Characterization of DPA@Ag / Cu NCs DPA@Ag / Cu NCs fluorescent probe
[0043] Scanning electron microscopy (SEM), transmission electron microscopy (TEM), ultraviolet-visible absorption spec...
Embodiment 2
[0045] Ag in buffer + detection
[0046] Different concentrations of Ag + (0.05, 0.1, 0.5, 1, 5, 15, 30, 50, 80, 100, 200, 500, 800, 100, 1200, 1500µM) were added to the prepared DPA@Ag / Cu NCs fluorescent probe solution, Afterwards, phthalic acid-hydrochloric acid buffer solution was added to adjust the pH of the system to 2.8, and after incubation at room temperature for 30 min, the fluorescence emission spectrum in the range of 450-650 nm was collected at an excitation wavelength of 353 nm, and a standard curve was drawn. The results are as follows: Figure 11 shown. When Ag + concentration in the range of 0-1500µM, with the Ag + As the concentration increases, the fluorescence intensity of the system decreases gradually, and the Ag + At high concentrations, fluorescence quenching gradually slows down. Figure 11 are different concentrations of Ag + The relationship diagram of the fluorescence change caused by it, the inset is the corresponding standard curve, and the...
Embodiment 3
[0052] Experimental condition optimization
[0053] Change the UV lamp irradiation time in the preparation method of Example 1 (other conditions are the same as Example 1), and the results show that irradiation 6h is the best time ( Figure 10 a).
[0054] Changing DPA and AgNO 3 The dosage ratio and Cu(NO 3 ) 2 The dosage (other conditions are the same as Example 1), the results show that [Ag + ]:[DPA]=1:4( Figure 10 b) and [Cu 2+ ]=2.5 mM( Figure 10 c), the fluorescence intensity of the prepared DPA@Ag / Cu NCs fluorescent probe is stronger.
[0055] The effect of changing different buffer solutions and their pH on the fluorescence change of the detection system (other conditions are the same as in Example 2), DPA@Ag / Cu NCs solution and Ag + In Britton-Robinson buffer solution (B.R buffer solution), phthalic acid-hydrochloric acid buffer solution (KHP-HCl buffer solution), glycine-hydrochloric acid buffer solution (Gly-HCl buffer solution), disodium hydrogen phosphat...
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