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A kind of method and its application of establishing dse and amf and plant symbiosis system

A symbiotic system, plant technology, applied in the fields of microbiology, ecology, and mycorrhizology, to achieve the effect of easy operation

Active Publication Date: 2021-10-12
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In many studies, there are few reports on the methods and applications of establishing AMF-DSE-plant symbiosis system

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Cultivation of Exophila pisciphila ACCC32496 in DSE-Maize Symbiotic System

[0043] 1. Strain activation and cultivation

[0044] After activating the Exophyllium ichthyophila strains from the frozen test tubes, they were respectively inoculated into plates containing potato sucrose agar medium, and cultured at 28°C for 45 days in the dark.

[0045] 2. Cultivation of sterile corn seedlings

[0046] Use 75% ethanol and 10% sodium hypochlorite solution to disinfect the surface of corn seeds, first soak the seeds in 75% ethanol for 30-60 seconds, then disinfect with 10% sodium hypochlorite solution for 10 minutes, and finally rinse with sterile water 4-5 times , Soak in sterile water for 20 minutes to make the seeds fully absorb water and swell. Inoculate the fully water-absorbed and swollen seeds into a sterile petri dish lined with sterile water soaked with sterilized filter paper, add a small amount of sterile water, and treat in the dark for 3 days in a light incubat...

Embodiment 2

[0057] Cultivation of Funneliformis mosseae in AMF-Maize Symbiotic System

[0058] 1. AMF "mycorrhizal soil" propagation

[0059] Mix the sandy soil agent containing the pure strain of Paleoma mosesi with sterilized sand (W / W3:1) and place it in a sterilized flowerpot. After transplanting the aseptic plant seedlings into the flowerpot, water it regularly. Sprinkle sterilized water to ensure the water requirements for the normal growth of plant roots, and cultivate for 90 days. Then, put the flowerpot under sunlight for 3 days to make the sand medium dry. Remove the aboveground part of the plant, take a small amount of fibrous roots for infection detection, and dry the remaining roots at 75°C, crush them, mix them in sand medium, put them in plastic bags for low temperature storage;

[0060] 2. Establishment of AMF and maize symbiosis system

[0061] (1) Inoculation of AMF and corn seedlings

[0062] Select a flowerpot with a height of 15cm and an outer diameter of 25cm to ...

Embodiment 3

[0065] Example 3: Culture of Exophila pisciphila ACCC32496 DSE-Moses Funneliformis mosseae AMF-Maize Symbiotic System

[0066] 1. Establishment of DSE-AMF-maize symbiosis system

[0067] (1) Inoculation of DSE and AMF with corn seedlings

[0068] The previous implementation steps were the same as in Example 1, so that the DSE-plant symbiont was successfully established. Then select a flower pot with a height of 15 cm and an outer diameter of 25 cm to sterilize and fill 5 kg of sterilized soil, sprinkle 45 g of P. Enter DSE to symbiotically cultivate two corn seedlings, and do not separate the two corn seedlings. Cultivated in a greenhouse at a temperature of about 25°C with natural light. Spray 50mL of 50% Hoagland nutrient solution every week. During the test, the water content of the soil was maintained at 15% by weighing and replenishing water, and the cultivation period was 90 days. At the same time, a blank control was established, and the number of parallels was con...

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Abstract

The invention discloses a method and application for establishing a symbiotic system between dark septate endophytes (Dark septate endophytes, DSE) and arbuscular mycorrhizal fungi (AMF) and plants, and mainly focuses on double inoculation. For practicability and operability, perlite with good air permeability is selected as the matrix, and modified Hoagland solution is added as nutrition, which meets the vegetative growth requirements for the establishment of the DSE-plant symbiosis system. After the DSE-plant symbiosis was formed, it was transplanted to sterilized soil containing AMF inoculants, and the modified Hoagland was added as a nutrient solution, which met the nutritional requirements for the establishment and later growth of the DSE-AMF-plant symbiosis system. The invention has the advantages of high efficiency, simplicity, easy operation, clarity, order and the like, and can be used for DSE-plant, AMF-plant, AMF-DSE-plant relationship research, DSE and AMF morphological research, suspected AMF, DSE strains Preliminary detection and application of DSE, AMF, DSE and AMF vaccinations.

Description

technical field [0001] The invention belongs to the technical fields of mycorrhizology, microbiology and ecology, and relates to a method for establishing a symbiosis system between DSE and AMF and plants and its application, specifically to effectively establish dark-colored compartmentalized endophytic fungi and arbuscular mycorrhizal fungi and Method and application of plant symbiotic culture system. Background technique [0002] In nature, plant roots will form a mutually beneficial symbiosis with certain types of soil fungi - mycorrhizae. Mycorrhizae mainly include Arbuscular mycorrhizas (AM), Ectomycorrhizas (ECM), Ectoendo mycorrhizas (EEM), and Arbutoid mycorrhizas (ARM). ), Crystal orchid mycorrhizas (Monotropoid mycorrhizas, MM), European heather mycorrhizas (Ericoid mycorrhizas, ERM) and Orchid mycorrhizas (Orchid mycorrhizas, OM). [0003] Among them, Arbuscular mycorrhizal fungi (AMF) can co-exist with most terrestrial plants to form arbuscular mycorrhiza, mak...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/10A01G31/00A01G24/20
CPCA01G31/00A01G18/10A01G24/20
Inventor 湛方栋张光群范旭杪李天国何永美李明锐阎凯祖艳群
Owner YUNNAN AGRICULTURAL UNIVERSITY
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