Surface plasma enhanced fluorescence imaging sensor and measuring method thereof

Abstract

The invention discloses a surface plasma enhanced fluorescence imaging sensor and a measuring method thereof in the field of sensors and sensing technologies. The surface plasma enhanced fluorescenceimaging sensor comprises a prism, a glass substrate, an upper metal coating layer, a waveguide dielectric layer, a lower metal coating layer, a refractive index matching dielectric layer and a samplecell, wherein the glass substrate is mounted at the bottom of the prism; the upper metal coating layer is arranged at the bottom of the glass substrate; the waveguide dielectric layer is mounted at the bottom of the upper metal coating layer; and the lower metal coating layer is arranged at the bottom of the waveguide dielectric layer. According to the invention, the waveguide mode and the LRSPR can realize high-proportioned incident light energy coupling; the combination of the two modes further improves the energy of the incident light coupled into the evanescent wave, thereby further improving the field enhancement coefficient of the interface of the lower metal coating layer-refractive index matching dielectric layer and the intensity of the WCLRSPEF signal on the basis of the traditional LRSPR.

Description

technical field [0001] The invention relates to the field of sensors and sensing technologies, in particular to a surface plasmon enhanced fluorescence imaging sensor and a measurement method thereof. Background technique [0002] When light is irradiated on a fluorescently labeled biochemical molecule, the energy of the light causes some electrons around some nuclei in the fluorescent label to transition from the ground state to an unstable singlet state such as the first excited singlet state or the second excited singlet state, and then quickly return to the ground state , while emitting energy in the form of light, resulting in fluorescence. Under the condition that a p-polarized beam of a certain wavelength couples energy into the evanescent wave at the metal film-dielectric interface through a prism coupler, when the incident angle is fixed at the surface plasmon resonance (Surface Plasmon Resonance, referred to as SPR) angle, the interface The nearby electromagnetic ...

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Problems solved by technology

[0004] The purpose of the present invention is to provide a surface plasmon-enhanced fluorescence imaging sensor and its measurement method, to solve the defects of low sensor field enhancement coefficient and fluorescence intensity signal in the prior art

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