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Trace amoxicillin detection method taking Ag@Au as SERS substrate

A technology of amoxicillin and detection method, which is applied in the detection field of trace substances, can solve problems such as complicated methods, and achieve the effect of simple preparation method, good linear relationship and strong SERS intensity

Active Publication Date: 2020-04-10
GUANGXI UNIVERSITY OF TECHNOLOGY
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  • Abstract
  • Description
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Problems solved by technology

[0004] Chinese patent application 201810611514.9 discloses a method for preparing a SERS substrate of Au@Ag nanoparticles and a method for detecting glucose using the substrate. Although this patent application also uses bimetallic nanoparticles as the SERS substrate, it uses Au@Ag nanoparticle Particles are used as the substrate, Ag@Au nanoparticles are not used as the SERS substrate, and the method for preparing Au@Ag nanoparticles in this patent application is complicated, and the target object of the patent is glucose
Therefore, there is no technical solution for detecting trace amoxicillin using Ag@Au as a SERS substrate.

Method used

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  • Trace amoxicillin detection method taking Ag@Au as SERS substrate
  • Trace amoxicillin detection method taking Ag@Au as SERS substrate
  • Trace amoxicillin detection method taking Ag@Au as SERS substrate

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Embodiment 1

[0025] Embodiment 1: A method for detecting trace amoxicillin using Ag@Au as a SERS substrate, comprising the following steps:

[0026] (1) Preparation of AgNPs: 0.01M AgNO 3(Silver nitrate) aqueous solution 4mL and trisodium citrate 0.1M solution 6mL are mixed together, then add water to make the final volume of the reaction solution reach 40mL, the mixed solution is magnetically stirred for 10min, then add 4mL of freshly prepared 100mM NaBH 4 (Sodium borohydride) solution; subsequently, 1 mL of H 2 o 2 (Hydrogen peroxide) is quickly injected into the above solution, and the color of the reaction mixture changes to light yellow, red, green, and finally blue within 3-4 minutes. The final product AgNPs (nano silver) is collected after centrifugation at 10,000 rpm for about 30 minutes. Then repeatedly wash with deionized water, stand-by;

[0027] (2) Preparation of Ag@Au nanoparticles: Using AgNPs as template particles, Ag@Au core-shell nanocomposites were prepared at room te...

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Abstract

The invention relates to a trace amoxicillin detection method taking Ag@Au as an SERS substrate. According to the method, a simple seed mediation method is adopted to prepare bimetal Ag@Au nanoparticles with stable chemical properties, finally triangular AgNPs are converted into discoid Ag@Au nanoparticles, and the obtained discoid Ag@Au nanoparticles are taken as an SERS substrate for trace amoxicillin detection, thereby realizing trace detection of amoxicillin. According to the method, the bimetallic Ag@Au nanoparticles with uniform morphology and particle size and good dispersity are synthesized, and the Ag@Au nanoparticles have high sensitivity, good spectral reproducibility and high SERS intensity. Experimental results show that the substrate is simple in preparation method, good in dispersity and high in sensitivity, can be used for rapid detection of foods and medicines, and has a wide application prospect.

Description

technical field [0001] The invention relates to a method for detecting trace substances, in particular to a method for detecting trace amoxicillin using Ag@Au as a SERS substrate. Background technique [0002] The detection methods of amoxicillin currently reported include microbial detection, enzyme-linked immunoassay, high performance liquid chromatography with fluorescence detector, ultraviolet detector and mass spectrometry detector, and high performance liquid chromatography-tandem mass spectrometry. The sensitivity of high performance liquid chromatography is low, and the sample treatment needs to be purified by derivatization or solid phase extraction (SPE) column, which is cumbersome to operate. The specificity of amoxicillin detection is not strong, and all require SPE column purification, which is costly. Other methods also have disadvantages such as complicated sample pretreatment, low sensitivity, and time-consuming. [0003] Surface-enhanced Raman scattering (...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 冯军徐娅娟程昊李利军黄文艺卢浩
Owner GUANGXI UNIVERSITY OF TECHNOLOGY
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