Method for synthesizing clubroot resistant brassica napus through pyramid breeding and biotechnology

Abstract

The invention belongs to the technical field of brassica napus germplasm improvement, and discloses a method for synthesizing clubroot resistant brassica napus through pyramid breeding and a biotechnology. Through distant hybridization, a disease-resistant descendant strain of a clubroot resistant Chinese cabbage variety namely Wendingchunlei and clubroot resistant mid-maturity Chinese kale are used as resistant gene sources of clubroot; double 11 in susceptible brassica napus varieties is used as a recurrent parent, a real hybrid obtained through distant hybridization is used as a female parent, backcross is performed, plants containing Chinese cabbage and Chinese kale resistance are screened through a molecular marker IP13-2 and a molecular marker KASP1 resisting the clubroot in the Chinese kale, SSR markers covering complete genomes are used for performing background selection on descendant plants, and resistant plants having highest similarity to the recurrent parent are selected.According to the method disclosed by the invention, the molecular markers are used for performing auxiliary selection and acceleration of a breeding process of clubroot resistant brassica napus strains, a plurality of clubroot resistant genes are polymerized, the resistance of the brassica napus to the clubroot is increased, and the breeding process is accelerated.

Description

technical field [0001] The invention belongs to the technical field of rapeseed breeding, and discloses a method for synthesizing brassica napus resistant to clubroot by means of aggregation breeding and biotechnology. Background technique [0002] At present, the closest existing technology: As early as the late 19th century, clubroot began to occur widely in North America and Australia. Until the beginning of the 20th century, clubroot occurred widely in almost all continents, and it was also distributed in most parts of my country . It leads to 10%-15% yield reduction of cruciferous crops in the world every year, thus becoming one of the main factors restricting the production of cruciferous crops. Clubroot is a soil-borne disease caused by Plasmodium brassicae, and it is one of the major devastating diseases of widely cultivated cruciferous crops. It mainly harms the roots of plants and causes the formation of tumors in the roots of plants. Tumors will hinder the absorp...

AI Technical Summary

Problems solved by technology

[0007](1) The germplasm resources of clubroot resistance in Brassica napus are relatively scarce, and it is difficult to meet the requirements of clubroot resistance breeding in Brassica napus

[0008](2) The source of clubroot resistance in the existing Brassica napus materials is relatively single, and only a few physiological races are resistant, and the resistance declines

[0009](3) The traditional method of breeding clubroot resistant materials in Brassica napus is relatively slow

At present, most researchers are looking for the source of resistance from cabbage, but the source of the resistance gene is relatively single, and the resistance is easy to disappear. Therefore, it is necessary to broaden the source of resistance genes. It is very necessary to obtain resistance from cabbage. Genetic, enhanced persistence of disease resistance in Brassica napus

[0011]The traditional method of backcrossing is mainly based on field phenotype selection, the workload is very heavy, and it is easy to make mistakes, lacking accurate identification at the molecular level, and it is effective very slow

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