Compound with antibacterial effect, preparation method thereof and application thereof in cigarettes

A compound, tobacco technology, applied in the field of phytochemistry, achieves good antibacterial activity, simple preparation and operation process, and high purity

Active Publication Date: 2020-04-17
CHINA TOBACCO YUNNAN IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention isolates a new benzopyranolactone compound from the stem bark of a genetically improved tobacco variety (Yunyan-300) with high resistance to black shank, and no related reports have been seen on this compound so far

Method used

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  • Compound with antibacterial effect, preparation method thereof and application thereof in cigarettes
  • Compound with antibacterial effect, preparation method thereof and application thereof in cigarettes
  • Compound with antibacterial effect, preparation method thereof and application thereof in cigarettes

Examples

Experimental program
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Effect test

Embodiment 1

[0045] The tobacco samples were from Yuxi, Yunnan, and the variety was Yunyan-300.

[0046] The preparation method of benzopyranolactone compound of the present invention comprises the following steps:

[0047] Sampling 20 kg of tobacco stem bark, crushing and extracting 5 times with 95 wt% methanol aqueous solution for 24 hours each time, combining the extracts, filtering, and concentrating under reduced pressure to obtain 1.2 kg of extract. The weight ratio of the aqueous methanol solution to the tobacco stem bark is 3.

[0048] The obtained extract was subjected to molecular distillation and separation, and fractions at 170-210°C were collected to obtain 112 g of distillation products.

[0049] The distillation product obtained by molecular distillation was dissolved in pure methanol with a weight ratio of 2.0 times, and then mixed with 120 g of 100-mesh crude silica gel, and 0.6 kg of 160-mesh silica gel was packed into a column for silica gel column chromatography. The v...

Embodiment 2

[0053] The tobacco sample comes from Dali, Yunnan, and the variety is Yunyan-300. The tobacco stem bark is sampled 35kg and chopped, extracted 4 times with 95wt% ethanol aqueous solution, each extraction is 48h, the extracts are combined, filtered, and concentrated under reduced pressure to form an extract. Obtain extractum 2.63kg. The weight ratio of the ethanol aqueous solution to the tobacco stem bark is 2.

[0054] The obtained extract was subjected to molecular distillation and separation, and fractions at 170-210°C were collected to obtain 234 g of a distillation product.

[0055] The distillation product obtained by molecular distillation was dissolved in pure methanol with a weight ratio of 2.0 times, and then mixed with 250 g of 80-mesh crude silica gel, and 1.2 kg of 200-mesh silica gel was packed into a column for silica gel column chromatography. The volume ratio was 1:0, 20:1, 9:1, 8:2, 7:3, 6:4, 1:1, 1:2 chloroform-acetone solution for gradient elution, TLC moni...

Embodiment 3

[0059] The tobacco sample comes from Kunming, Yunnan, and the variety is Yunyan-300. Take 50kg of tobacco stem bark and grind it, and use 75wt% acetone aqueous solution to extract 3 times with ultrasound, each time for 72 hours. The extracts are combined, filtered, and concentrated under reduced pressure to form an extract. , to obtain extract 3.92kg. The weight ratio of the acetone aqueous solution to the tobacco stem bark is 4.

[0060] The extract was separated by molecular distillation, and the fractions at 170-210°C were collected to obtain 438g of distillation products. The part obtained by molecular distillation was dissolved in pure methanol with a weight ratio of 1.6 times, and then mixed with 400g of 90-mesh crude silica gel, and 2.4kg of 180-mesh Silica gel column was packed for silica gel column chromatography, using a chloroform-acetone gradient with a volume ratio of 1:0, 20:1, 9:1, 8:2, 7:3, 6:4, 1:1, 1:2 Elution, TLC monitoring Merge the same polar fractions t...

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Abstract

The invention belongs to the technical field of phytochemistry, and particularly relates to a compound with an antibacterial effect, a preparation method of the compound and application of the compound in cigarettes. The molecular formula of the compound is C16H18O4, and the compound has a structural formula disclosed in the invention. The invention also discloses the preparation method and application of the compound. According to the preparation method, a new benzopyran lactone compound is separated from genetically modified tobacco stem bark with high black shank resistance for the first time for the first time. The compound shows good antibacterial activity, and the antibacterial rates on escherichia coli, staphylococcus aureus and the like all reach 95% or above.

Description

technical field [0001] The invention belongs to the technical field of phytochemistry, and in particular relates to a compound with antibacterial effect, its preparation method and its use in cigarettes. Background technique [0002] Tobacco is an important commercial crop grown all over the world. Tobacco contains rich chemical components and complex biological activities. More than 4,000 monomeric chemical substances have been identified from tobacco, and many components have not yet been identified. In addition to being mainly used as raw materials in the tobacco industry, the whole plant can also be used as a pesticide and insecticide; it can also be used medicinally for anesthesia, sweating, sedation and emetics. In addition, a variety of valuable chemical components can be extracted from it, and leading compounds with development and utilization value can be found therefrom. [0003] Benzopyranolactones are a class of important heterocyclic compounds with pharmaceut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/58A01N43/16A01P3/00A01P1/00B65D65/38
CPCC07D311/58A01N43/16B65D65/38
Inventor 李银科李雪梅杨光宇米其利孔维松向海英曾婉俐高茜李晶胡秋芬张承明
Owner CHINA TOBACCO YUNNAN IND
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