Preparation method and applications of chitosan polypeptide derivative self-cross-linking hydrogel taking oxidized hyaluronic acid as cross-linking agent

A technology for oxidizing hyaluronic acid and chitosan polypeptides, applied in the field of polymer chemistry, can solve problems such as hindering development and poor solubility, and achieve the effects of no biological toxicity, excellent swelling performance, and good blood compatibility.

Inactive Publication Date: 2020-04-17
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the poor solubility of chitosan in water and other organic solvents hinders its development

Method used

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  • Preparation method and applications of chitosan polypeptide derivative self-cross-linking hydrogel taking oxidized hyaluronic acid as cross-linking agent
  • Preparation method and applications of chitosan polypeptide derivative self-cross-linking hydrogel taking oxidized hyaluronic acid as cross-linking agent
  • Preparation method and applications of chitosan polypeptide derivative self-cross-linking hydrogel taking oxidized hyaluronic acid as cross-linking agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1. Preparation of carboxymethyl chitosan (CMCS):

[0042](1) Alkalinization of chitosan (CS): Measure 20 mL of distilled water in a beaker, put into a magnetic stirrer to stir, add 20 g of NaOH solid, and prepare a 50% NaOH solution as an alkalizing agent for CS. After the NaOH solution was completely cooled, slowly add 20 g CS to it in batches, and stir with a glass rod while adding, until it became fluffy, and finally put it in a -5 °C refrigerator for 24 h.

[0043] (2) Preparation reaction of CMCS: Take out the CS that has been frozen for 24 hours from the refrigerator, and after the temperature rises to room temperature, weigh 30 g of basified CS and add it to a three-necked flask, then add 200 mL of isopropanol as a dispersion medium, and stir mechanically 30min and adjust the temperature to 55°C. Then weigh 5g of chloroacetic acid and add it into the three-necked flask. After 15min, weigh 10g of chloroacetic acid and add it slowly, set the reaction time to 6h, a...

Embodiment 2

[0055] Preparation of hydrogel:

[0056] The difference between this example and Example 1 is that 0.8mL of OHA solution was added to 2.0mL of CMCS / COP solution, stirred thoroughly and left to form a gel, which was coded as CCHA-4.

Embodiment 3

[0058] Preparation of hydrogel:

[0059] The difference between this example and Example 1 is that 1.2mL of OHA solution was added to 2.0mL of CMCS / COP solution, stirred thoroughly and left to form a gel, coded as CCHA-6.

[0060] Carry out infrared characterization to raw material HA in embodiment 1, the prepared OHA and the CCHA-6 hydrogel prepared in embodiment 3, see the attached Figure 5 , the results showed that the amino groups in CMCS / COP and the aldehyde groups in OHA had a cross-linking reaction, and were connected in the form of amide bonds to form a composite hydrogel. The CCHA-6 hydrogel prepared in Example 3 is characterized by a scanning electron microscope, and the scanning electron microscope picture of the hydrogel sample section is shown in the attached Figure 6 , the results show that this hydrogel has a uniformly distributed pore structure, good continuity, smooth and flat pore structure, and a certain thickness. This excellent three-dimensional space ...

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Abstract

The invention belongs to the field of polymer chemistry, and particularly relates to a preparation method and applications of a chitosan polypeptide derivative self-cross-linking hydrogel taking oxidized hyaluronic acid as a cross-linking agent. The preparation method comprises the following steps: (1) preparing oxidized hyaluronic acid; (2) preparing carboxymethyl chitosan; (3) preparing carboxymethyl chitosan / collagen peptide; and (4) respectively preparing a carboxymethyl chitosan / collagen peptide solution and an oxidized hyaluronic acid solution, uniformly mixing at a certain temperature,and carrying out self-cross-linking to obtain the hydrogel. According to the preparation method, the grafting reaction of carboxymethyl chitosan and collagen peptide is catalyzed by using transglutaminase as a catalyst, so that the process is simple, the reaction conditions are mild, the covalent bonds formed by the catalytic reaction are firm, the biotoxicity is avoided, the repeatability is good, and good economic value is achieved.

Description

technical field [0001] The invention belongs to the field of polymer chemistry, and in particular relates to a preparation method and application of chitosan polypeptide derivative self-crosslinking hydrogel with oxidized hyaluronic acid as a crosslinking agent. Background technique [0002] Hydrogel is a polymer material that can swell in water but is insoluble in water. Through intermolecular cross-linking, a three-dimensional network structure is formed, and its molecular chain has hydrophilic groups. With the development of modern science and technology, hydrogels have many applications in the fields of controlled release of drugs, tissue engineering, and regeneration of organs. Hydrogel dressing is a new type of medical dressing that is similar to the extracellular matrix of human tissue and has good biocompatibility. The surface of the hydrogel dressing is soft, easy to fit the wound well, can provide a good moist healing environment for the wound, can absorb the exud...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/24C08J3/075C08G81/00C08L87/00C08L5/08A61L24/08A61L24/10A61L24/00A61L26/00
CPCA61L24/001A61L24/0031A61L24/08A61L24/102A61L26/0023A61L26/0033A61L26/0061A61L26/008A61L2400/04C08J3/075C08J3/24C08J2387/00C08J2405/08C08L5/08
Inventor 樊李红罗鹏峰徐文艳杨晓爽张畅胡婉庆
Owner WUHAN UNIV OF TECH
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