Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bioreactor and method for preparing pectin oligosaccharide and natural food preservative of pectin oligosaccharide

A bioreactor, pectin oligosaccharide technology, applied in specific-purpose bioreactors/fermenters, biochemical equipment and methods, enzyme production/bioreactors, etc., can solve temperature and pH difficult to control, substrates Axial diffusion inhibition, easy loss of immobilized enzymes, etc., to improve reusability, facilitate efficient recovery, and facilitate industrialization

Active Publication Date: 2020-04-17
SHENYANG AGRI UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The batch stirred reactor has the problems that the carrier is easily damaged by the shear force of the stirring blades, and the immobilized enzyme is easily lost during the recovery process; the packed bed reactor has the problems of easy blockage in the bed, difficult control of temperature and pH, and axial flow of the substrate. Disadvantages such as diffusion inhibition and troublesome cleaning and replacement; fluidized bed reactor overcomes the shortcomings of the packed bed temperature and pH value, which are difficult to control and easy to block, but must maintain a certain flow rate during the production process, which is costly and difficult to scale up in industry. ; Membrane bioreactors are also widely used enzyme reactors, which can eliminate the inhibition of the product on the enzyme reaction, but are prone to "leakage" and membrane fouling; in view of the above problems, a shaft-imitation membrane type is designed The production of antibacterial oligosaccharides and the development of natural preservatives in biobioreactors are of great significance for promoting the industrial production of natural food preservatives

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bioreactor and method for preparing pectin oligosaccharide and natural food preservative of pectin oligosaccharide
  • Bioreactor and method for preparing pectin oligosaccharide and natural food preservative of pectin oligosaccharide
  • Bioreactor and method for preparing pectin oligosaccharide and natural food preservative of pectin oligosaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] A kind of bioreactor for preparing pectin oligosaccharides, its structural schematic diagram is shown in figure 1 , including a substrate tank 1, an enzyme carrier 3, a temperature control system 4 and a drive 2;

[0067] A driver 2 is arranged on the top of the substrate tank 1 , an enzyme carrier 3 is arranged at the lower end of the driver 2 , and a temperature control system 4 is arranged on the periphery of the substrate tank 1 .

[0068] The enzyme carrier 3 is filled with graded-pore carbon microsphere carrier immobilized pectinase; and the graded filling is carried out according to the macroscopic particle size of the graded-pore carbon microsphere carrier immobilized pectinase 3cm:5mm=3:7.

[0069] Wherein, the schematic diagram of the structure of the enzyme carrier is shown in figure 2 , which includes an enzyme carrier housing 301 and a through hole 302 arranged on the enzyme carrier housing, wherein the charcoal microsphere carrier with a macroscopic part...

Embodiment 2

[0084] Production of pectin oligosaccharides with the bioreactor for preparing pectin oligosaccharides in embodiment 1, its specific method is as follows:

[0085] step 1:

[0086] Take an aqueous pectin solution with a mass percentage concentration of 0.1%, adjust the pH to 4, add it to the substrate tank of the bioreactor for preparing pectin oligosaccharides, and immerse the enzyme carrier provided at the lower end of the actuator in the aqueous pectin solution. Adjust the temperature control system so that the temperature of the pectin aqueous solution in the substrate tank is 40°C, and the immobilized pectinase in the fixed pectinase in the carbon microsphere carrier filled in the enzyme carrier is contacted with the pectin for 4 hours to decompose and react to obtain Pectin breakdown products;

[0087] Step 2:

[0088] The decomposed pectin product is treated with a hollow fiber membrane to recover pectin oligosaccharides with a molecular weight of 3000 daltons or less...

Embodiment 3

[0090] Production of pectin oligosaccharides with the bioreactor for preparing pectin oligosaccharides in Example 1, its specific method is as follows: Step 1:

[0091] Take an aqueous pectin solution with a mass percentage concentration of 0.5%, adjust the pH to 4, add it to the substrate tank of the bioreactor for preparing pectin oligosaccharides, and immerse the enzyme carrier provided at the lower end of the actuator in the aqueous pectin solution. Adjust the temperature control system so that the temperature of the pectin aqueous solution in the substrate tank is 45°C, and the immobilized pectinase in the fixed pectinase in the carbon microsphere carrier filled in the enzyme carrier is contacted with the pectin for 8 hours to decompose and react to obtain Pectin breakdown products;

[0092] Step 2:

[0093] The pectin decomposition product is subjected to ultrafiltration and nanofiltration treatment with a size-exclusion filtration membrane, and pectin oligosaccharides wi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
pore sizeaaaaaaaaaa
specific surface areaaaaaaaaaaa
Login to View More

Abstract

The invention discloses a bioreactor and a method for preparing pectin oligosaccharide and a natural food preservative of the pectin oligosaccharide, belonging to the field of development and application of natural food preservatives. According to the bioreactor for preparing the pectin oligosaccharide, a driver is arranged at the top of a substrate tank of the bioreactor; an enzyme carrier is connected to the lower end of the driver, and a temperature control system is arranged at the periphery of the substrate tank; and the enzyme carrier is filled with graded porous carbon microsphere carrier-immobilized pectinase. The method comprises the following steps: adjusting the pH value of an aqueous pectin solution to 3-5, putting the aqueous pectin solution into the substrate tank, carrying out a decomposition reaction for 30-24 hours at a temperature of 30-50 DEG C and a rotating speed of 120-160 rpm to obtain a pectin decomposer, and purifying the pectin decomposer to obtain the pectinoligosaccharide with a molecular weight of 200-3000 Daltons and an average polymerization degree of 2-15. The oligosaccharide-based natural food preservative is prepared by taking the pectin oligosaccharide as a basic raw material, and the preservative is green, safe and good in antibacterial spectrum adaptability. The bioreactor adopted for producing the pectin oligosaccharide has the advantagesof convenience, high efficiency, low cost, easiness in implementation, environmental protection and high yield.

Description

technical field [0001] The invention specifically relates to a bioreactor and method for preparing pectin oligosaccharides and a natural food preservative, and belongs to the technical field of development and application of natural food preservatives. Background technique [0002] At present, the food preservatives on the market are still mainly chemical synthetics. In recent years, there have been frequent incidents of chemical preservatives endangering human health. Based on the improvement of consumers' awareness of self-health management and resistance to synthetic food additives, natural food additives Into people's field of vision, but the current natural food preservatives have weak antibacterial properties, narrow antibacterial spectrum, and low usability. Therefore, the development of an efficient natural food preservative that is beneficial to the human body and has antiseptic ability has become an urgent problem to be solved in the field of food additives at home...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/40C12M1/38C12M1/12C12M1/00C12N11/14C12P19/14C12P19/00A23L3/3562
CPCC12M21/18C12M23/02C12M41/12C12M47/12C12N11/14C12P19/14C12P19/00C12N9/18C12N9/2402C12N9/88C12Y301/01011C12Y302/01015C12Y402/02002A23L3/3562
Inventor 李拖平张金凤徐昱峰李苏红
Owner SHENYANG AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com