Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Prussian blue-based nanoprobe and preparation method and application thereof

A nanoprobe, Prussian blue technology, applied in the field of biosensors, can solve the problem of lack of ONOO-selectivity, and achieve the effect of improving poor penetration depth, no detection and imaging, and high sensitivity

Active Publication Date: 2020-04-17
HUNAN UNIV
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Recently, Qu and co-workers reported the detection of ROS in vivo by MRI, but this MRI probe lacks the ability to detect ONOO - selectivity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Prussian blue-based nanoprobe and preparation method and application thereof
  • Prussian blue-based nanoprobe and preparation method and application thereof
  • Prussian blue-based nanoprobe and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1 Preparation of simple PB nanoprobe:

[0060] 5mL containing K 4 [Fe(CN) 6 ] (1mM) of citric acid solution (30mM) was slowly added to 5mL containing FeCl 3 (1 mM) in HCl solution (25 mM); at room temperature, and then vigorously stirred for 60 minutes; then, the obtained PB nanoparticles were centrifuged and washed 3 times with ultrapure water to remove excess unreacted ions, and stored in 4°C.

[0061] figure 2 Be the transmission electron microscope figure of embodiment 1 gained PB, from figure 2 It can be seen that the particle size of the synthesized PB is about 35nm.

Embodiment 2

[0062] Example 2 PB detects ONOO - proficiency testing

[0063] Using the PB obtained in Example 1, quickly 10 μL of different concentrations of ONOO - Solutions (eg 0, 40, 80, 120, 160 and 200 μM) were added to 190 μL of PBS (pH=7.4) containing PB solution;

[0064] The final concentration of PB was 3.8 μg / mL, which was tested after 10 minutes by UV-Vis absorption; and ONOO was selected - Two samples at concentrations of 0 and 200 μM were tested for particle size change by DLS.

[0065] Additionally, using ONOO - Donor 5-amino-3-(4-morpholinyl)-1,2,3-oxadiazole hydrochloride (SIN-1) confirmed that the response of PB was indeed caused by ONOO - caused by other substances in the reaction system. After incubation of PB with SIN-1 in PBS (0.1 mg / mL, pH=7.4) for 5 hours, the uptake of SIN-1-treated PB (3 μg / mL) was greatly reduced compared to untreated PB.

[0066] image 3 Detect ONOO for PB - The change in particle size after DLS, from image 3 It can be seen that the s...

Embodiment 3

[0070] Example 3PB detects ONOO by photoacoustic imaging - proficiency testing

[0071] Using the PB obtained in Example 1, quickly add 20 μL of different concentrations of ONOO - Solutions (eg 0, 40, 80, 120, 160 and 200 μM) were added to 380 μL of PBS (pH=7.4) containing PB solution. The final concentration of PB was 9.5 μg / mL, and photoacoustic imaging was performed 10 min later.

[0072] Figure 6 Detection of ONOO for PB photoacoustics - proficiency testing from Figure 6 It can be seen that the photoacoustic signal of PB in the near-infrared region increases with the ONOO - decrease with increasing concentration.

[0073] Figure 7 Detection of ONOO for PB photoacoustics - Photoacoustic signal at 710nm and ONOO - The relationship between concentrations, from Figure 7 It can be seen that PB detects ONOO - , the photoacoustic signal at 710nm and ONOO - Concentrations are negatively correlated.

[0074] Analysis: Thanks to ONOO -The near-infrared absorption o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Particle sizeaaaaaaaaaa
Particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention discloses a prussian blue-based nanoprobe, and a preparation method and an application of the prussian blue-based nanoprobe in detection and imaging of peroxynitrite ionsand belongs to the technical field of biosensors. The nano-probe is any one of a PB nano-probe and a CuS-coated PB nano-probe, and the structural formula of the nano-probe is shown in the specification, through the two nanoprobes, PAI and MRI dual-mode imaging is realized by changing a valence state of iron ions and the structure of PB under the oxidation effect of ONOO <->, detection and imaging with high sensitivity, good specificity and no penetration depth limitation on ONOO <-> are realized, and problems of poor penetration depth and low specificity of a conventional probe are greatly improved. The method is advantaged in that the PB nanoprobe and the CuS-coated PB nanoprobe are prepared through a simple method, the preparation process is short, operation is simple, and cost is low.

Description

technical field [0001] The invention belongs to the technical field of biosensors, and relates to a Prussian blue-based nanoprobe and its preparation method and application, in particular to a Prussian blue (PB) nanoprobe based on biomarker peroxynitrite ion (ONOO - ) detection and imaging applications. Background technique [0002] Peroxynitrite ion (ONOO - ) is a typical reactive oxygen species (ROS), composed of nitric oxide (NO) and superoxide anion radicals (O 2 -· ), which plays a crucial role in physiological and pathological processes. ONOO - The high reactivity and the presence of a large number of targets lead to its short biological half-life (<10ms), although ONOO - Plays a crucial role in signal transduction and antibacterial activity, but excess ONOO - Key components of cells are damaged, including lipids, DNA, proteins, thiols, and iron-sulfur clusters, and the accumulation of cellular damage eventually leads to apoptosis and necrosis. There is growi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/31G01N21/01G01N21/17G01N24/08
CPCG01N21/01G01N21/1702G01N21/31G01N24/08
Inventor 宋国胜陈芳芳张晓兵
Owner HUNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com