Prussian blue-based nanoprobe and preparation method and application thereof

A nanoprobe, Prussian blue technology, applied in the field of biosensors, can solve the problem of lack of ONOO-selectivity, and achieve the effect of improving poor penetration depth, no detection and imaging, and high sensitivity

Active Publication Date: 2020-04-17
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Recently, Qu and co-workers reported the detection of ROS in vivo by M

Method used

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  • Prussian blue-based nanoprobe and preparation method and application thereof
  • Prussian blue-based nanoprobe and preparation method and application thereof
  • Prussian blue-based nanoprobe and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1 Preparation of simple PB nanoprobe:

[0060] 5mL containing K 4 [Fe(CN) 6 ] (1mM) of citric acid solution (30mM) was slowly added to 5mL containing FeCl 3 (1 mM) in HCl solution (25 mM); at room temperature, and then vigorously stirred for 60 minutes; then, the obtained PB nanoparticles were centrifuged and washed 3 times with ultrapure water to remove excess unreacted ions, and stored in 4°C.

[0061] figure 2 Be the transmission electron microscope figure of embodiment 1 gained PB, from figure 2 It can be seen that the particle size of the synthesized PB is about 35nm.

Embodiment 2

[0062] Example 2 PB detects ONOO - proficiency testing

[0063] Using the PB obtained in Example 1, quickly 10 μL of different concentrations of ONOO - Solutions (eg 0, 40, 80, 120, 160 and 200 μM) were added to 190 μL of PBS (pH=7.4) containing PB solution;

[0064] The final concentration of PB was 3.8 μg / mL, which was tested after 10 minutes by UV-Vis absorption; and ONOO was selected - Two samples at concentrations of 0 and 200 μM were tested for particle size change by DLS.

[0065] Additionally, using ONOO - Donor 5-amino-3-(4-morpholinyl)-1,2,3-oxadiazole hydrochloride (SIN-1) confirmed that the response of PB was indeed caused by ONOO - caused by other substances in the reaction system. After incubation of PB with SIN-1 in PBS (0.1 mg / mL, pH=7.4) for 5 hours, the uptake of SIN-1-treated PB (3 μg / mL) was greatly reduced compared to untreated PB.

[0066] image 3 Detect ONOO for PB - The change in particle size after DLS, from image 3 It can be seen that the s...

Embodiment 3

[0070] Example 3PB detects ONOO by photoacoustic imaging - proficiency testing

[0071] Using the PB obtained in Example 1, quickly add 20 μL of different concentrations of ONOO - Solutions (eg 0, 40, 80, 120, 160 and 200 μM) were added to 380 μL of PBS (pH=7.4) containing PB solution. The final concentration of PB was 9.5 μg / mL, and photoacoustic imaging was performed 10 min later.

[0072] Figure 6 Detection of ONOO for PB photoacoustics - proficiency testing from Figure 6 It can be seen that the photoacoustic signal of PB in the near-infrared region increases with the ONOO - decrease with increasing concentration.

[0073] Figure 7 Detection of ONOO for PB photoacoustics - Photoacoustic signal at 710nm and ONOO - The relationship between concentrations, from Figure 7 It can be seen that PB detects ONOO - , the photoacoustic signal at 710nm and ONOO - Concentrations are negatively correlated.

[0074] Analysis: Thanks to ONOO -The near-infrared absorption o...

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Abstract

The invention discloses a prussian blue-based nanoprobe, and a preparation method and an application of the prussian blue-based nanoprobe in detection and imaging of peroxynitrite ionsand belongs to the technical field of biosensors. The nano-probe is any one of a PB nano-probe and a CuS-coated PB nano-probe, and the structural formula of the nano-probe is shown in the specification, through the two nanoprobes, PAI and MRI dual-mode imaging is realized by changing a valence state of iron ions and the structure of PB under the oxidation effect of ONOO <->, detection and imaging with high sensitivity, good specificity and no penetration depth limitation on ONOO <-> are realized, and problems of poor penetration depth and low specificity of a conventional probe are greatly improved. The method is advantaged in that the PB nanoprobe and the CuS-coated PB nanoprobe are prepared through a simple method, the preparation process is short, operation is simple, and cost is low.

Description

technical field [0001] The invention belongs to the technical field of biosensors, and relates to a Prussian blue-based nanoprobe and its preparation method and application, in particular to a Prussian blue (PB) nanoprobe based on biomarker peroxynitrite ion (ONOO - ) detection and imaging applications. Background technique [0002] Peroxynitrite ion (ONOO - ) is a typical reactive oxygen species (ROS), composed of nitric oxide (NO) and superoxide anion radicals (O 2 -· ), which plays a crucial role in physiological and pathological processes. ONOO - The high reactivity and the presence of a large number of targets lead to its short biological half-life (<10ms), although ONOO - Plays a crucial role in signal transduction and antibacterial activity, but excess ONOO - Key components of cells are damaged, including lipids, DNA, proteins, thiols, and iron-sulfur clusters, and the accumulation of cellular damage eventually leads to apoptosis and necrosis. There is growi...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/01G01N21/17G01N24/08
CPCG01N21/01G01N21/1702G01N21/31G01N24/08
Inventor 宋国胜陈芳芳张晓兵
Owner HUNAN UNIV
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