Metal composites for enhanced imaging
一种结合元件、金纳米颗粒的技术，应用在生物化学设备和方法、微生物的测定/检验、抵抗媒介传播的疾病等方向，能够解决荧光信号不足、荧光输出强度限制等问题
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Embodiment 1
[0120] Embodiment 1: the solution phase method of the thin film preparation of silver on gold
[0121] This example describes the preparation of thin films of silver on gold. Materials used in this example included glass slides and quartz slides purchased from Fisher Scientific that were rinsed with acetone, IPA, and methanol before use. Silicon wafers with native oxide layers were purchased from the Center for Integrated Systems, Stanford University. Poly(vinyl chloride) coverslips were purchased from Ted Pella, Inc. Sylgard 184 was purchased from Dow Corning and cured by standard procedures. Chlorauric acid trihydrate, hydroxylamine hydrochloride, sodium borohydride and glucose were purchased from Sigma-Aldrich. Ammonium hydroxide (30% ammonia) was purchased from Fisher Chemicals. Plasmon resonances of cAg / Au films on glass substrates were measured by UV-vis-NIR absorption spectroscopy with a Cary 300 spectrophotometer, background corrected for any glass contribution. ...
Embodiment 2
[0129] Example 2: Seeding of gold precursors onto unmodified substrates
[0130] The preparation of Ag / Au thin films involves three steps: seeding of gold precursors, reduction to Au 0 clusters, and through Ag 1+ Reductive selective growth ( Picture 1-1 and Figure 1-2 Views (a)-(e)). Seeding is achieved by adding ammonium hydroxide to a chloroauric acid solution containing the substrate of choice. After ammonium hydroxide addition, clear yellow acidic HAuCl 4 The solution immediately became cloudy and orange-yellow, with a pH of about 9. Discovered Au 3+ The deposition rate of the substance on the substrate is faster. Increasing the exposure time from one minute to 20 minutes did not significantly affect the density or size of the gold seeds immobilized on the substrate.
[0131] The seeding density of Au seeds depends on the HAuCl the substrate is immersed in before precipitation with ammonium hydroxide 4 the initial concentration. For inorganic substrates such ...
Embodiment 3
[0132] Example 3: Solution Phase Reduction of Gold Precipitate Precursors
[0133] After deposition by Au seeds precipitated onto the chosen substrate, the substrate is immersed in a 0.1 mM to 100 mM sodium borohydride solution, which results in Au 0 Rapid formation of nanoparticles, evidenced by a pale pink-purple change in the substrate. Atomic force microscopy revealed the formation of Au NPs with a diameter of 5–10 nm ( Picture 1-1 (b)), and UV / Vis absorption spectroscopy reveals a weak surface plasmon resonance at 525 nm typical for Au NPs in this size range.
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