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A Korean Pseudomonas strain and its screening method and application

A technology of pseudomonas and strains, applied in the field of microorganisms, can solve the problems of unsatisfactory phosphorus-dissolving effect of strains, and achieve the effects of good phosphorus-dissolving ability, saving planting costs, and reducing pollution problems.

Active Publication Date: 2022-03-01
辽宁土木启生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the researches are based on traditional strain screening techniques, and the phosphorus-dissolving effect of the screened strains is not satisfactory.

Method used

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  • A Korean Pseudomonas strain and its screening method and application
  • A Korean Pseudomonas strain and its screening method and application
  • A Korean Pseudomonas strain and its screening method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1 Pseudomonas Korea (Pseudomonas koreansis) screening and identification.

[0053] 1. Pseudomonas Korea (Pseudomonas koreansis) Screening for P623-9:

[0054] The antagonistic bacteria Pseudomonas korean P623-9 of the present invention is screened from rhizosphere soil of continuous cropping tomato seedlings.

[0055] 1.1 Preparation of Pseudomonas culture medium.

[0056] 1.1.1 Phosphorous bacteria enrichment medium: take the following components by weighing: (NH 4 ) 2 SO0.5g, NaCl 0.5g, KCl 0.3g, MgSO 4 7H 2 0 0.3g, Ca(PO 4 ) 2 10g, FeS0 4 7H 2 O 0.03g, MnSO 4 4H 2 0 0.03g, 1.0g of phosphate rock powder, 10g of glucose, 1000ml of distilled water, adjust the pH to 7.0-7.5, sterilize under high temperature and high pressure at 121℃ for 20min, and set aside.

[0057] 1.1.2 Separation and screening of solid medium: (NH 4 ) 2 SO 4 0.1g, KCL0.2g, MgSO 4 7H 2 0 0.25g, NaCl 0.5g, glucose 10g, phosphate rock powder 5g, agar powder 18g, dilute to 100...

Embodiment 2

[0068] Example 2 Phosphorus-solubilizing ability experiment of Pseudomonas Koreana P623-9.

[0069] (1) Qualitative phosphorus-dissolving effect test (primary screening): Determination of phosphorus-dissolving ability under solid culture conditions: the phosphorus-dissolving circle method is used. The purified strains were picked and placed on solid medium culture dishes. Each treatment was repeated three times, and cultured in a 28 °C incubator for 7 days. The ratio of the diameter of the phosphorus-dissolving circle to the diameter of the colony (D / d) was measured. Determine the strength of each strain's phosphorus-solubilizing ability, such as figure 1 shown.

[0070] (2) Quantitative phosphorus-dissolving ability test (molybdenum-antimony anti-colorimetric method): Pick the strains with larger phosphorus-dissolving circles and good colony growth in the qualitative phosphorus-dissolving effect test and inoculate them into 100 mL inorganic phosphorus liquid medium (such as...

Embodiment 3

[0071] Example 3 Comparative experiment on the phosphorus-solubilizing ability of Pseudomonas korean P623-9 on tomato, a facility vegetable.

[0072] 1. Test sample: Pseudomonas Korea P623-9.

[0073] 2. Field test crops:

[0074] Facility vegetables: tomato, pepper, cucumber, melon.

[0075] 3. Test methods and procedures.

[0076] (1) Activation of strains and preparation of bacterial solution

[0077] Pick a single colony on the Pseudomonas korean P623-9 plate and inoculate it in 5ml of liquid LB medium, and inoculate it in 100ml of liquid LB medium with shaking at 30°C for 24 hours, then inoculate it in 100ml of liquid LB medium respectively, and culture it with shaking at 30°C for 24 hours to obtain the culture solution, count (The number of bacteria is 1.1×10 8 CFU / ml), spare.

[0078] (2) Field test

[0079] After the four crops were planted, when the second true leaf unfolded, 5-fold diluted bacterial solution was added to the roots, and controls were set up for ...

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Abstract

The invention provides a strain of Pseudomonas Korea ( Pseudomonas koreansis ) strain, named P623‑9, has been preserved in Guangdong Microbial Culture Collection Center (GDMCC), address: 5th Floor, Building 59, Compound, No. 100 Xianlie Middle Road, Guangzhou City, Zip Code: 510070, preservation number GDMCC NO.60806, The date of deposit was October 14, 2019; and the application of the strain of Pseudomonas korean in improving soil phosphorus utilization and growth promotion of facility vegetables was disclosed. The Pseudomonas Korean strain P623-9 provided by the present invention has a phosphorus-dissolving capacity as high as 496 mg / L, has good phosphorus-dissolving ability, and can convert insoluble phosphate phosphorus such as fixed phosphorus in soil into soluble phosphorus that is easy for vegetables to absorb and utilize , reduce the amount of phosphorus fertilizer application, promote the growth of vegetables, increase the yield by more than 15% during the application process, save planting costs, and reduce pollution problems.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to Pseudomonas korean and its screening method and application. Background technique [0002] Soil is the material basis for the sustainable development of agriculture and an important nutrient source for crop growth. Phosphorus is one of the important nutrient elements for plant growth. 74% of the cultivated land in my country is deficient in phosphorus, and the phosphorus fertilizer applied to the soil is only 5%-25% % utilization rate, most of the phosphorus has become insoluble phosphate that cannot be absorbed, and it is not easy for plants to directly absorb and utilize it. Bacterial strains, researchers use biotechnology to improve the availability of soil phosphorus to plants by breeding phosphorus-dissolving microorganisms. At present, most studies are based on traditional strain screening techniques, and the phosphorus-dissolving effect of the screened strains ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02A01G7/06C12R1/38
CPCC12N1/20C12N1/02A01G7/06C12R2001/38C12N1/205
Inventor 吴红艳池景良于淼冯健冯敏陈飞郭玲玲王智学
Owner 辽宁土木启生物科技有限公司
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