Improved culture medium for determining biological value of polyoxin and preparation method thereof
A bio-potency, polyantimycin technology, applied in biochemical equipment and methods, microorganism-based methods, and microbial determination/inspection, etc., can solve the problem of blurred edges, long incubation time of indicator bacteria, and clarity of inhibition zone. Not high problems, to save time, dissolve faster, and achieve the effect of high clarity
Active Publication Date: 2020-04-24
陕西麦可罗生物科技有限公司
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Problems solved by technology
[0006] In order to solve the above-mentioned problems existing in the determination of the biological potency of polydactin in the prior art, the present invention provides an improved medium for measuring the biological potency of polydactin and its preparation method, which can effectively solve the problem that the clarity of the inhibition zone is not high. , fuzzy edges, and the indicator bacteria have been cultured for too long
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[0037] This example discloses a specific embodiment for preparing a polyoxin biological potency determination medium, which includes the following steps:
[0038] 1) Preparation of vegetable juice: Take 250 g of tomatoes, wash with pure water, remove the skin, chop them, add 500 mL of pure water, boil for 15 minutes, filter with 4-6 layers of gauze to obtain tomato juice, put it at room temperature and seal it, 4℃ Keep in the refrigerator for later use. Vegetable juice should be sterilized under ultraviolet light for 30-45 min before use.
[0039] 2) Preparation of the upper medium: 1.0% sucrose, 0.05% L-asparagine, 0.05% potassium dihydrogen phosphate, 1.6% vegetable juice, 1.5% agar, the balance is pure water, heat and boil for 15-30 minutes, use 4 Filter with 6 layers of gauze, adjust the pH between 5.9 and 6.3 after placing it at room temperature, and sub-pack to obtain the upper medium. Among them, the vegetable juice is added before measuring the biological potency of the u...
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The invention relates to an improved culture medium for determining the biological value of polyoxin and a preparation method thereof. The improved culture medium is composed of an upper-layer culturemedium and a lower-layer culture medium. The upper-layer culture medium is prepared from 0.5%-3% of cane sugar, 0.01%-0.2% of L-asparagine, 0.01%-0.2% of monopotassium phosphate, 0.5%-5% of vegetablejuice, and 10%-20% of agar, with the balance being pure water. The lower-layer culture medium is prepared from 0.5% to 3% of cane sugar, 0.01% to 0.2% of L-asparagine, 0.01% to 0.2% of monopotassiumphosphate and 10% to 20% of agar, with the balance being pure water. Compared with an original PDA culture medium, the preparation method of the invention is simple, an indicator bacterium colony cultured by the culture medium of the invention grows better and more stably, culture time is shortened, the edge of an inhibition zone is clearer and is easy to observe and measure, and cost is effectively saved.
Description
Technical field [0001] The invention belongs to the technical field of microbial fermentation, and specifically relates to an improved polyoxin biological potency determination culture medium and a preparation method thereof. Background technique [0002] Polyoxin is a metabolite produced by Streptomyces aureus and belongs to a broad-spectrum antibiotic fungicide. Polyoxin has a good systemic conduction effect. Its mechanism of action is to interfere with the biosynthesis of chitin in the cell wall of the bacteria, which prevents the biosynthesis of the cell wall of the bacteria and causes the death of the bacteria. In addition, it also inhibits the production of spores and lesions. Expanded role. Polyoxin has different targets because of its different components. It is mainly composed of a and b components. It is mainly used to prevent apple leaf spotting, ring streak, pear black spot, and grape gray mold. There are more than ten crop diseases such as powdery mildew, downy mil...
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IPC IPC(8): C12Q1/18C12N1/14C12R1/645
CPCC12Q1/18C12N1/14
Inventor 姚亚丽翁婧郑鹏飞杨宏勃潘忠成李蒲民
Owner 陕西麦可罗生物科技有限公司