Soybean peptide with alpha-glucosidase activity inhibition function, preparation method and application

A technology of glucosidase and soybean peptide, applied in the field of soybean peptide and preparation, can solve the problems of incapable of industrial scale preparation, complicated separation and purification process, low product activity, etc. powerful effect

Pending Publication Date: 2020-05-01
广州合诚实业有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the current preparation technology of glucosidase-inhibiting active peptides is still in the laboratory research stage, focusing on enzymatic hydrolysis process, preliminary exploration of product activity and identification of active peptides. Some key link technologies have not yet been resolved, mainly including: (1) Preparation process , especially the separation and purification process is complicated, and it cannot be prepared on an industrial scale; (2) the activity of the product is low, and it has not been confirmed whether it still has activity after passing through the gastrointestinal system in the human body
This type of product has not yet been seen in the market

Method used

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  • Soybean peptide with alpha-glucosidase activity inhibition function, preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] (1) Dispersion and dissolution of soybean protein isolate powder: use deionized water equivalent to 9 times the quality of soybean protein isolate powder (model SD-300, Linyi Shansong Biological Products Co., Ltd., the same below) to prepare soybean protein isolate powder For protein slurry, disperse and mix the slurry at a shear rate of 2000rpm for 20min, and raise the temperature to 55°C;

[0049] (2) Alkaline protease enzymolysis: the pH value of the protein slurry dispersed and mixed in step (1) is adjusted to 8.0 with a NaOH solution with a concentration of 2mol / L, and an alkaline solution equivalent to 1.5% of the quality of soybean protein isolate powder is added. Protease Alcalase 2.4L, keep stirring at a constant temperature of 55°C (at a speed of 36rpm) for enzymolysis for 6h;

[0050] (3) Adjust the pH of the enzymolysis solution, inactivate the enzyme, and centrifuge: adjust the pH value of the enzymolysis solution in step (2) to 4.5 with a concentration of ...

Embodiment 2

[0056] (1) Dispersion and dissolution of soybean protein isolate powder: use deionized water equivalent to 12 times the quality of soybean protein isolate powder to prepare protein slurry from soybean protein isolate powder, disperse and mix the slurry for 10 minutes at a shear rate of 3000 rpm, and heat up to 50°C;

[0057] (2) Alkaline protease hydrolysis: adjust the pH value of the protein slurry dispersed and mixed in step (1) to 9.0 with a concentration of 4mol / L NaOH solution, and add alkaline protease equivalent to 0.5% of the quality of soybean protein isolate powder Alcalase 2.4L, keep stirring at a constant temperature of 50°C (36rpm) for enzymolysis for 10h;

[0058] (3) Adjust the pH of the enzymolysis solution, inactivate the enzyme, and centrifuge: adjust the pH value of the enzymolysis solution in step (2) to 4.2 with a concentration of 4mol / L HCl solution, raise the temperature to 95°C for 10 minutes to inactivate the enzyme, and pump it into the horizontal Th...

Embodiment 3

[0064] (1) Dispersion and dissolution of soybean protein isolate powder: use deionized water equivalent to 6 times the quality of soybean protein isolate powder to prepare protein slurry from soybean protein isolate powder, disperse and mix the slurry for 15 minutes at a shear rate of 2500 rpm, and heat up to 50°C;

[0065] (2) Alkaline protease enzymatic hydrolysis: adjust the pH value of the protein slurry dispersed and mixed in step (1) to 7.0 with a concentration of 1mol / L NaOH solution, and add alkaline protease equivalent to 3.0% of the mass of soybean protein isolate Alcalase 2.4L, keep stirring at a constant temperature of 60°C (at a speed of 36rpm) for 2 hours;

[0066] (3) Adjust the pH of the enzymolysis solution, inactivate the enzyme, and centrifuge: adjust the pH value of the enzymolysis solution in step (2) to 4.8 with a concentration of 2mol / L HCl solution, raise the temperature to 85°C for 30min to inactivate the enzyme, and pump it into the horizontal The sc...

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Abstract

The invention discloses a soybean peptide with an alpha-glucosidase activity inhibition function, a preparation method and application. Soybean protein isolate powder is taken as a raw material, and the soybean peptide with the strong alpha-glucosidase activity inhibition function is prepared through alkaline protease enzymolysis, isoelectric point enzyme deactivation centrifugal separation, pepsin enzymolysis, ultrafiltration, nanofiltration and drying. The technology aims at preparing the soybean peptide with the strong alpha-glucosidase activity inhibition function, the target peptide is efficiently obtained by fully utilizing an enzyme combination technology and integrating separation technologies such as isoelectric point centrifugal separation, ultrafiltration and nanofiltration, theproduction technology process is scientific, compact and energy-saving, the preparation method is easy to operate, and the soybean peptide with the alpha-glucosidase activity inhibition function issuitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of high value-added deep processing and utilization of by-products of agricultural products, and in particular relates to a soybean peptide with the activity of inhibiting α-glucosidase, its preparation method and application. Background technique [0002] At present, diabetes mellitus (DM) is a metabolic disorder caused by hyperglycemia due to insufficient insulin secretion. It is a disease that seriously endangers human health. Cause endocrine disorders in the body. At present, diabetes has become one of the three major chronic diseases that threaten human health. The number of patients worldwide exceeds 300 million, and it is estimated that the number of patients will reach more than 500 million by 2030. In China, about 114 million people suffer from diabetes. China has become the largest diabetes country in the world. More than 90% of diabetes mellitus belongs to type Ⅱ diabetes mellitus. Its main path...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34A23L33/18
CPCC12P21/06C07K1/34A23L33/18A23V2002/00A23V2200/328A23V2250/55Y02P20/10
Inventor 周雪松曾建新蒋文真
Owner 广州合诚实业有限公司
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