Zero-mode waveguide hole wall modification method and zero-mode waveguide hole structure

A zero-mode waveguide and modification method technology, applied in the field of zero-mode waveguide hole wall modification and zero-mode waveguide hole structure, can solve the problems of fluorescence signal detection interference, nucleotides, low signal-to-noise ratio, etc. Small free nucleotides, sensitive detection, enhanced fluorescence effect

Active Publication Date: 2020-05-08
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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Problems solved by technology

[0004] However, during the sequencing process, there are many free nucleotides in the pores, which interfere with the detection of fluorescent signals and cause low signal-to-noise ratios; the closer the fluorescence is excited to the metal pore wall, the weaker the effect will be, and it will occur when it is completely close fluorescence quenching

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  • Zero-mode waveguide hole wall modification method and zero-mode waveguide hole structure
  • Zero-mode waveguide hole wall modification method and zero-mode waveguide hole structure
  • Zero-mode waveguide hole wall modification method and zero-mode waveguide hole structure

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[0036] Below, the present invention will be further described in conjunction with the accompanying drawings and specific implementation methods. It should be noted that, under the premise of not conflicting, the various embodiments described below or the technical features can be combined arbitrarily to form new embodiments. .

[0037] The invention provides a method for modifying the wall of a zero-mode waveguide hole, such as Figure 1-6 shown, including the following steps:

[0038] S1. Cover the hole wall of the zero-mode waveguide hole with a polymer 104 and cure it; wherein, the hole wall of the zero-mode waveguide hole includes a metal cladding layer 103 and an optical fiber waveguide layer 102 before covering. In one embodiment, such as figure 2 , 3 As shown, the metal cladding layer 103 and the optical fiber waveguide layer 102 form a hole whose wall is the hole of the metal cladding layer 103 and whose bottom end is the hole of the optical fiber waveguide layer 1...

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Abstract

The invention provides a zero-mode waveguide hole wall modification method. The method comprises the following steps: covering a polymer; irradiating ultraviolet light on the surface of a metal covering layer to form a first chemical bond; and stripping the polymer. The invention also relates to a zero-mode waveguide hole structure. The hole wall of the zero-mode waveguide hole is covered with thepolymer, and ultraviolet light irradiates the surface of the metal covering layer for bonding to form a high-refractive-index non-reflective first chemical bond; the in-hole volume of the zero-mode waveguide hole can be reduced by increasing the deposition thickness of the first chemical bond of the high-refractive-index non-reflective material, so that free nucleotides in the hole can be significantly reduced, and the signal-to-noise ratio can be improved. Besides, the position of excited fluorescence can be far away from the metal wall of the zero-mode waveguide hole by depositing the firstchemical bond of a high-refractive-index non-reflective material in the hole, so that the fluorescence cannot be weakened or even quenched, and the detection is more sensitive while the fluorescenceeffect is enhanced.

Description

technical field [0001] The invention relates to the field of micro-nano processing technology, in particular to a method for modifying the hole wall of a zero-mode waveguide hole and a zero-mode waveguide hole structure. Background technique [0002] Currently, real-time single-molecule sequencing is achieved using zero-mode waveguide pores (ZMW). are fluorescently labeled phosphate-linked nucleotides that allow visualization of uninterrupted DNA polymerization. [0003] ZMWs nanostructures consist of dense arrays of holes deposited on transparent substrates such as silicon dioxide. Each ZMW becomes a nanophotonic visualization chamber for recording a single polymerization reaction, offering a detection volume of only 10 -21 Lift. This volume is a 1000-fold improvement over diffraction-limited confocal microscopy, enabling the visualization of single nucleotide incorporation events in the context of diffusion of fluorescently labeled nucleotides. In addition to reducing ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G02B6/10G02B6/13C12M1/00C12Q1/6869
CPCG02B6/107G02B6/13C12Q1/6869C12Q2565/631
Inventor 周连群付博文郭振李传宇李金泽张威李超姚佳张芷齐
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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