Medical recombinant collagen spray and preparation method thereof

A recombinant collagen, a certain amount of technology, applied in medical science, bandages, etc., can solve the problems of rejection, uncertain molecular weight, wide distribution, and hidden dangers of animal viruses, etc., to achieve easy portability, promote wound repair, and accelerate The effect of epidermal cell migration

Inactive Publication Date: 2020-05-15
南京艾澜德生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Common collagen is isolated and extracted from animal tissues such as bovine bone, bovine hide, fish scale, fish skin, and pig skin. There are hidden dangers of animal viruses. Disadvantages and deficiencies such as wide distribution

Method used

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  • Medical recombinant collagen spray and preparation method thereof
  • Medical recombinant collagen spray and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Embodiment 1 Preparation of medical recombinant collagen spray 1

[0024] Weigh 7.164g disodium hydrogen phosphate (Na2HPO4.12H2O) and dissolve it in 100ml deionized water. Weigh 3.121g of sodium dihydrogen phosphate (NaH2PO4.2H2O) and dissolve it in 100ml of deionized water. Mix 61ml of disodium hydrogen phosphate aqueous solution with 39ml of sodium dihydrogen phosphate aqueous solution to obtain a 0.2M / L buffer solution with a pH of 7.0. Weigh 0.07g of recombinant human collagen, 0.01g of hyaluronic acid, 0.9g of sodium chloride, and 5.42ml of glycerin into 94.18ml of buffer solution, and fully dissolve on a magnetic stirrer at 1000r / min at 37°C. Weigh 0.06g of methylparaben and 0.01g of ethylparaben in 400ul of phenoxyethanol, and dissolve completely in a 37°C water bath. Add the phenoxyethanol dissolved in methylparaben and ethylparaben to the previously prepared recombinant human collagen and hyaluronic acid solution, stir well, and then ultrasonically debubble ...

Embodiment 2

[0025] Embodiment 2 Preparation 2 of medical recombinant collagen spray

[0026] Weigh 7.164g disodium hydrogen phosphate (Na2HPO4.12H2O) and dissolve it in 100ml deionized water. Weigh 3.121g of sodium dihydrogen phosphate (NaH2PO4.2H2O) and dissolve it in 100ml of deionized water. Mix 61ml of disodium hydrogen phosphate aqueous solution with 39ml of sodium dihydrogen phosphate aqueous solution to obtain a 0.2M / L buffer solution with a pH of 7.0. Weigh 0.1g of recombinant human collagen, 0.02g of hyaluronic acid, 0.9g of sodium chloride, and 3.24ml of glycerin into 96.396ml of buffer solution, and fully dissolve on a magnetic stirrer at 1000r / min at 37°C. Weigh 0.03g of methylparaben and 0.03g of ethylparaben, dissolve them in 364ul of phenoxyethanol, and dissolve them completely in a 37°C water bath. Add the phenoxyethanol dissolved in methylparaben and ethylparaben to the previously prepared recombinant human collagen and hyaluronic acid solution, stir well, and then ultr...

Embodiment 3

[0027] Embodiment 3 Preparation of medical recombinant collagen spray 3

[0028]Weigh 7.164g disodium hydrogen phosphate (Na2HPO4.12H2O) and dissolve it in 100ml deionized water. Weigh 3.121g of sodium dihydrogen phosphate (NaH2PO4.2H2O) and dissolve it in 100ml of deionized water. Mix 61ml of disodium hydrogen phosphate aqueous solution with 39ml of sodium dihydrogen phosphate aqueous solution to obtain a 0.2M / L buffer solution with a pH of 7.0. Weigh 0.1g of recombinant human collagen, 0.022g of hyaluronic acid, 0.9g of sodium chloride, and 4.10ml of glycerin into 95.52ml of buffer solution, and fully dissolve on a magnetic stirrer at 1000r / min at 37°C. Weigh 0.01g of methylparaben and 0.06g of ethylparaben in 380ul of phenoxyethanol, and dissolve completely in a 37°C water bath. Add the phenoxyethanol dissolved in methylparaben and ethylparaben to the previously prepared recombinant human collagen and hyaluronic acid solution, stir well, and then ultrasonically debubble a...

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Abstract

The invention provides a medical recombinant collagen spray and a preparation method thereof and belongs to the technical field of skin injury dressings and skin repairing. The hydrogel provided by the invention is mainly prepared by compounding recombinant collagen, hyaluronic acid, sodium chloride, glycerinum, p-hydroxy benzoate, phenoxyethanol, sodium dihydrogen phosphate and a disodium hydrogen phosphate buffer solution. The medical recombinant collagen spray provided by the invention has good moisture retention, provides a continuous wet environment for a wound, and is capable of promoting wound healing and protecting newly generated wound tissue, reconstructing skin barrier functions and alleviating partial dryness, pain and itching symptoms. The medical recombinant collagen spray issimple in production process, low in production cost and applicable to large-scale production. In addition, compared with dressings, ointments and the like of other modes, the spray provided by the invention has the advantages of being easy to carry over, free of swab smearing and safe and convenient to use.

Description

technical field [0001] The invention relates to a medical recombinant collagen spray and a preparation method thereof, in particular to the technical fields of skin injury dressing and skin repair. Background technique [0002] Collagen is the main component of the extracellular matrix and the most abundant protein in animals. All multicellular animals are rich in collagen, so collagen has good biocompatibility. Collagen has a unique tissue structure and biological function, which plays an important role in maintaining the normal physiological functions and repairing of cells, tissues, and organs. It has a high affinity, good biodegradation safety, and can be degraded and absorbed. Biomaterials with excellent performance have been widely used in the medical field. Common collagen is isolated and extracted from animal tissues such as bovine bone, bovine hide, fish scale, fish skin, and pig skin. There are hidden dangers of animal viruses. Disadvantages and deficiencies such...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L26/00
CPCA61L26/0004A61L26/0023A61L26/0033A61L26/0076A61L26/009C08L5/08
Inventor 杨树林王子勋邓爱鹏
Owner 南京艾澜德生物科技有限公司
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