Application of corydalis saxicola and preparation thereof in preparing medicines for treating non-alcoholic fatty liver diseases

A fatty liver disease, non-alcoholic technology, applied in the field of medicine, can solve the problems of no effective drug treatment and complex pathogenesis, and achieve the effect of expanding clinical indications, preventing liver damage, improving biochemical indicators and imaging evaluation results

Active Publication Date: 2020-05-22
JIANGSU HONGDIAN RES INST OF TRADITIONAL CHINESE MEDICINE IND CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The pathogenesis of non-alcoholic fatty liver disease is very complicated, and there is no recognized effective drug treatment at present, so the development of effective drugs based on the research on the pathogenesis still needs to continue to work

Method used

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  • Application of corydalis saxicola and preparation thereof in preparing medicines for treating non-alcoholic fatty liver diseases
  • Application of corydalis saxicola and preparation thereof in preparing medicines for treating non-alcoholic fatty liver diseases
  • Application of corydalis saxicola and preparation thereof in preparing medicines for treating non-alcoholic fatty liver diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Preparation of total alkaloids of Litectin

[0035] (1) Take 30kg of Yanhuanglian decoction pieces, add 10 times the volume concentration of 75% ethanol and soak for 1 hour;

[0036] (2) Then heat and reflux for extraction three times, each time for 2 hours, and combine the extracts;

[0037] (3) Recover as much ethanol as possible and concentrate to 1:1.3 (Rectinus rhizome W: Concentrate V) to obtain a concentrate;

[0038] (4) Take the above concentrated solution and add 1% hydrochloric acid which is 4 times the amount of the raw material (Rectinus root decoction pieces), put it in an acid-resistant multifunctional extraction tank and heat and reflux for extraction for 1 hour;

[0039] (5) Adjust the extract with 40% sodium hydroxide and keep the pH value to 8, then concentrate to a relative density of 1.06-1.08 (60°C), refrigerate the concentrate, let it stand for 48 hours, and filter; filter the precipitate for use Heat 1% hydrochloric acid 16 times the ...

Embodiment 2

[0043] Example 2 Observation of curative effect of Yanhuanglian total alkaloid capsules on cell experiments.

[0044] 1. Test drug: the total alkaloid extract (CSBTA) of Litectinus chinensis prepared in Example 1.

[0045] 2. Cytotoxicity experiment

[0046] 1.1 Experimental instrument: MULTSKAN Sky full-wavelength microplate reader (Thermo Scientific, USA)

[0047] 1.2 Reagents: MTT cell proliferation and cytotoxicity detection kit (article number: KGA321, Jiangsu Kaiji Biotechnology Co., Ltd.); total alkaloids of Litectinum prepared in Example 1;

[0048] 1.3 Experimental method: Add 100 µL / well of cells in a 96-well plate (about 1×10 4 ), set at 37℃, 5%CO 2 Cell culture incubator for 24 hours. Aspirate the medium of all wells, use the blank medium without adding serum to configure CSTBA (100ug / mL) solution, dilute it to seven concentrations of 50, 25, 12.5 and so on, and mix the blank medium with Each concentration of medium was added to a 96-well plate and placed at 3...

Embodiment 3

[0050] Example 3 Observation of the curative effect of total alkaloids of Litectinum chinensis on hypolipidemic effect of hyperlipidemia model rats

[0051] 1. Experimental modeling

[0052] 1.1 Experimental animals: male C57BL / 6 black mice, 4 weeks old, weighing about 19g, purchased from the Comparative Medicine Center of Yangzhou University.

[0053] 1.2 Feed: 60% high-fat feed, fructose, and normal feed were purchased from Nantong Trophy Feed Technology Co., Ltd.

[0054] 1.3 Reagents: the total alkaloids extract of Litectinus rhizome prepared in Example 1.

[0055] 1.4 Experimental method: Take 35 male C57BL / 6 small black mice, 4 weeks old, after one week of adaptive feeding, divide the mice into two initial groups randomly, and feed them with normal diet or high-fat (60% fat) high-sugar Diet (20% fructose). After feeding for 10 weeks, the mice were randomly divided into model groups, total alkaloids of Litectin (25mg / kg), total alkaloids of Litectin (100mg / kg), metformin...

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Abstract

The present invention discloses an application of corydalis saxicola and a preparation thereof in treating non-alcoholic fatty liver diseases and/or non-alcoholic steatohepatitis. According to the invention, through a large amount of experimental screening, it is found that corydalis saxicola total alkaloids have good effects of reducing synthesis in fatty acid cells, and can reduce the levels ofTG, TC, LDL cholesterol, HDL cholesterol and free fatty acid induced by HFD. Animal anatomical experiments show that the corydalis saxicola total alkaloids can effectively reduce the weight of livers,and pathological sections show that liver tissues are well protected. In addition clinical experiments show that corydalis saxicola total alkaloid capsules can obviously improve biochemical indexes and imaging evaluation results of fatty liver patients. The drug-induced liver injury experimental results show that the corydalis saxicola total alkali capsules can obviously improve drug-induced liver injury and have the effect of preventing liver injury.

Description

technical field [0001] The present invention relates to a new application of Yanhuanglian and Yanhuanglian total alkaloid capsules, in particular to the application of Yanhuanglian and its Yanhuanglian total alkaloid capsules in the treatment of nonalcoholic fatty liver disease and nonalcoholic steatohepatitis. field of medical technology. Background technique [0002] Non-alcoholic fatty liver disease (NAFLD) is defined as hepatic steatosis confirmed by imaging and liver histology, and other causes of hepatic steatosis have been excluded, such as heavy drinking, long-term use of pro-adipogenic drugs or Single-gene genetic disorders, etc., according to their clinical manifestations, mainly include hepatic steatosis, non-alcoholic steato-hepatitis (non-alcoholic steato-hepatitis, NASH) and liver cirrhosis. The pathogenesis of non-alcoholic fatty liver disease is very complicated, and there is no recognized effective drug treatment at present, so the development of effective ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/66A61K9/48A61P1/16
CPCA61K36/66A61K9/48A61P1/16A61K2236/333A61K2236/39A61K2236/53A61K2236/51A61K36/505
Inventor 成俊刁和芳黄芳郑艳萍赵开军
Owner JIANGSU HONGDIAN RES INST OF TRADITIONAL CHINESE MEDICINE IND CO LTD
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