A kind of hyaluronic acid sustained-release filler and its preparation method
A technology of hyaluronic acid and sodium hyaluronate, which is applied in the field of biomaterials and medical cosmetology, can solve problems such as easy formation of depressions or collapses, limited effect of collagen regeneration, and insufficient tissue support, so as to reduce costs and save time , the effect of reducing the number of injections
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[0037] There are many preparation methods for absorbable polymer porous microspheres, such as: double emulsion solvent evaporation method, suspension polymerization method, dispersion polymerization method, seed swelling method, microfluidic polymerization method, etc. are recorded. The invention adopts the disclosed double-emulsion solvent evaporation method to prepare porous microspheres.
[0038] The absorbable polymer porous microspheres can be PCL porous microspheres, or can be replaced by in vivo degradable or water-soluble polymer materials such as PLA, PLGA, PGA, PVA, P-TMC;
[0039] Only one type of cross-linked sodium hyaluronate and small molecular sodium hyaluronate can be added, and can be replaced by surfactants / density increasing agents with excellent water solubility and biocompatibility such as CMC, MC, and Tween;
[0040] PBS buffer can be replaced by normal saline, nutrients such as growth factors can be added, and anesthetic ingredients such as lidocaine c...
Embodiment 1
[0044] Example 1 Preparation of PCL microspheres and porous microspheres with a specific surface area of 318m 2 / g
Embodiment 2
[0045] Example 2 Preparation of PLA microspheres and porous microspheres with a specific surface area of 238m 2 / g
[0046] Embodiment 1:
[0047] PCL porous microspheres were used as absorbable polymer porous microspheres. The recipe is as follows:
[0048] The mass ratio is 30%, the particle size is 25~50 microns, the average pore size is 1 micron PCL porous microspheres, 1% cross-linked sodium hyaluronate, 2% small molecular sodium hyaluronate, 67% PBS buffer configuration into an injection filling product, the external sodium hyaluronate slowly degrades and forms collagen outside the microspheres, and then the sodium hyaluronate in the back hole slowly degrades, inducing collagen fibers to grow into the hole and form a scaffold-like structure, and finally PCL Porous microspheres degrade slowly. The small molecule sodium hyaluronate on the outside of the microspheres was completely degraded within 2 months, and the cross-linked sodium hyaluronate outside the pores of t...
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Abstract
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