High-throughput sequencing detection method for pathogenic microorganisms with full-process quality control

A technology of pathogenic microorganisms and detection methods, which is applied in the field of high-throughput sequencing and detection of pathogenic microorganisms for whole-process quality control, to achieve the effect of reducing pollution, reducing false positives, and rapid clinical detection

Active Publication Date: 2020-05-22
HUGOBIOTECH BEIJING CO LTD +2
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Problems solved by technology

However, there is currently no systematic, full-process quality control ...

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  • High-throughput sequencing detection method for pathogenic microorganisms with full-process quality control
  • High-throughput sequencing detection method for pathogenic microorganisms with full-process quality control
  • High-throughput sequencing detection method for pathogenic microorganisms with full-process quality control

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Embodiment Construction

[0066] The preferred embodiments of the present invention will be described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

[0067] Compared with the traditional pathogenic microorganism diagnosis technology and the most similar existing technology, this solution provides a systematic whole-process quality control mNGS solution for the detection of pathogenic microorganisms for the clinical needs of infectious disease diagnosis of pathogenic microorganisms, as follows:

[0068] The embodiment of the present invention provides a high-throughput sequencing detection method for pathogenic microorganisms in the whole process of quality control, such as figure 1 shown, including:

[0069] Step 1: adding an internal reference to the first microbial sample to be detected to obtain a second micro...

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Abstract

The invention provides a high-throughput sequencing detection method for pathogenic microorganisms with full-process quality control. The method comprises the following steps: adding an internal reference into a first to-be-detected microorganism sample to obtain a second to-be-detected microorganism sample; extracting DNA in the second to-be-detected microorganism sample, a preset positive control sample and a blank control sample respectively; constructing a sequencing library for the extracted DNA samples by adopting a pathogenic microorganism detection kit respectively; sequencing the sequencing library based on a second-generation sequencing platform, performing quality control processing on original sequencing data in a sequencing result, and removing a sequence compared to a host genome to obtain to-be-compared data; and comparing the to-be-compared data based on a pre-established comparison database, and determining main pathogenic microorganisms according to a comparison result. Rapid clinical detection of the pathogenic microorganisms which do not need to be predicted, do not have preference and have high throughput for infectious diseases is realized, meanwhile, full-process quality control of a system is realized, and false positive is reduced.

Description

technical field [0001] The invention relates to the technical field of clinical detection of infectious diseases, in particular to a high-throughput sequencing detection method for pathogenic microorganisms in the whole process of quality control. Background technique [0002] Traditional diagnostic techniques for pathogenic microorganisms in infectious diseases include microscopy, culture, and serological methods. Microscopy is less sensitive unless the sample has a high disease burden and is not specific. The sensitivity of culture varies with pathogens, especially some difficult-to-culture microorganisms with complex nutritional requirements, such as cryptococcus, the most common 3 neuroinvasive fungi can be cultured on standard, variable sensitivity bacterial media , as for more rare fungi, specific media are required; for example, herpes simplex virus can only be cultured from CSF in up to 5% of cases of herpes simplex virus encephalitis, and antibiotic treatment can a...

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Application Information

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IPC IPC(8): C12Q1/6869G16B30/10
CPCC12Q1/6869G16B30/10C12Q2535/122C12Q2525/191C12Q2531/113C12Q2537/165
Inventor 夏涵
Owner HUGOBIOTECH BEIJING CO LTD
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