Preparation method of composite electro-spun tissue engineering scaffold material, engineering scaffold material and application thereof

A tissue engineering scaffold and electrospinning technology, which is applied in textiles and papermaking, rayon manufacturing, and conjugated synthetic polymer artificial filaments, etc., can solve degradation product cells, tissue damage, structure vulnerable to external force damage, and related complications and other issues, to achieve good mechanical properties and biocompatibility, improve bioavailability, and proper degradation speed

A tissue engineering scaffold and electrospinning technology, which is applied in textiles and papermaking, rayon manufacturing, and conjugated synthetic polymer artificial filaments, etc., can solve degradation product cells, tissue damage, structure vulnerable to external force damage, and related complications and other issues, to achieve good mechanical properties and biocompatibility, improve bioavailability, and proper degradation speed

CN111188102AActive Publication Date: 2020-05-22THE SIXTH AFFILIATED HOSPITAL OF SUN YAT SEN UNIV +1
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  • Preparation method of composite electro-spun tissue engineering scaffold material, engineering scaffold material and application thereof
  • Preparation method of composite electro-spun tissue engineering scaffold material, engineering scaffold material and application thereof
  • Preparation method of composite electro-spun tissue engineering scaffold material, engineering scaffold material and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A preparation method for a composite electrospun tissue engineering scaffold material, comprising the steps of:

[0029] S1. Preparation of poly(3-hydroxybutyrate-4-hydroxybutyrate) / L-polylactic acid electrospun fibers: Weigh 40 mg of medical-grade P34HB powder (purity greater than 99.5%) and 40 mg of medical-grade L-lactic acid The powder was dissolved in a composite solvent of 1000 mL of dichloromethane and dimethylformamide (DMF) (the volume ratio of dichloromethane and dimethylformamide was 3:7), stirred and dissolved by a magnetic stirrer for 2 hours, and the temperature was 60 ℃, stir evenly, and make P34HB / PLLA solution.

[0030] S2. Preparation of polyhydroxyalkanoate / L-polylactic acid composite electrospun fiber mixed solution loaded with epirubicin: Weigh 5 mg of epirubicin and add poly(3-hydroxybutyrate-4- Hydroxybutyrate) / L-polylactic acid electrospun fiber solution, continue magnetic stirring until the epirubicin is completely dissolved.

[0031] S3. Add ...

Embodiment 2

[0033]A preparation method for a composite electrospun tissue engineering scaffold material, comprising the steps of:

[0034] S1. Preparation of poly(3-hydroxybutyrate-4-hydroxybutyrate) / poly-L-lactic acid electrospun fibers: Weigh 40 mg of medical-grade P34HB powder (purity greater than 99.5%) and 32 mg of medical-grade poly-L-lactic acid The powder was dissolved in a composite solvent of 1000 mL of dichloromethane and dimethylformamide (DMF) (the volume ratio of dichloromethane and dimethylformamide was 6:4), stirred and dissolved by a magnetic stirrer for 2 hours, and the temperature was 60 ℃, stir evenly, and make P34HB / PLLA solution.

[0035] S2. Preparation of polyhydroxyalkanoate / L-polylactic acid composite electrospun fiber mixed solution loaded with epirubicin: Weigh 4 mg of epirubicin and add poly(3-hydroxybutyrate-4- Hydroxybutyrate) / L-polylactic acid electrospun fiber solution, continue magnetic stirring until the epirubicin is completely dissolved.

[0036] S3....

Embodiment 3

[0038] A preparation method for a composite electrospun tissue engineering scaffold material, comprising the steps of:

[0039] S1. Preparation of poly(3-hydroxybutyrate-4-hydroxybutyrate) / L-lactic acid electrospun fibers: Weigh 40 mg of medical-grade P34HB powder (purity greater than 99.5%) and 48 mg of medical-grade L-lactic acid The powder was dissolved in a composite solvent of 1000 mL of dichloromethane and dimethylformamide (DMF) (the volume ratio of dichloromethane and dimethylformamide was 2:8), stirred and dissolved by a magnetic stirrer for 2 hours, and the temperature was 60 ℃, stir evenly, and make P34HB / PLLA solution.

[0040] S2. Preparation of polyhydroxyalkanoate / L-polylactic acid composite electrospun fiber mixed solution loaded with epirubicin: Weigh 6 mg of epirubicin and add poly(3-hydroxybutyrate-4- Hydroxybutyrate) / L-polylactic acid electrospun fiber solution, continue magnetic stirring until the epirubicin is completely dissolved.

[0041] S3. Add the ...

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Abstract

The invention discloses a preparation method of a composite electro-spun tissue engineering scaffold material, the engineering scaffold material and application thereof. The preparation method of thecomposite electro-spun tissue engineering scaffold material comprises the steps of adopting a poly (3-hydroxybutyrate-4-hydroxybutyrate) copolymer and levorotatory polylactic acid mixed material to prepare electro-spun fibers through an electrostatic spinning technology, and spinning epirubicin into a P34HB / PLLA fiber scaffold through mixed electro-spinning to prepare the epirubicin-loaded P34HB / PLLA composite electro-spun fibers. The epirubicin-loaded P34HB / PLLA composite electro-spun fibers of the composite electro-spun tissue engineering scaffold material prepared by the preparation methodhave good mechanical property and biocompatibility, proper degradation speed and excellent anti-tumor property, and have wide application prospect in the biomedical field of tumor treatment.

Description

technical field [0001] The invention relates to the field of medical biomaterials, more specifically, the invention relates to a preparation method of a composite electrospun tissue engineering scaffold material, an engineering scaffold material and applications thereof. Background technique [0002] Polyhydroxyalkanoate (PHA) is a linear saturated polyester whose fiber waste can be completely absorbed by the natural environment and has good biodegradability and biocompatibility, while poly 3-hydroxybutyrate / 4- Hydroxybutyrate (P34HB) is the latest generation of biodegradable materials in the PHA family. Fibrous scaffolds prepared based on electrospinning technology have a similar fiber structure to natural extracellular matrix (ECM), and thus are widely used in cell proliferation culture, drug delivery, biosensors and other fields. With the deepening of tissue engineering research, electrospinning technology continues to develop, and different spinning processes and method...

Claims

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Application Information

Patent Timeline
22 May 2020
Publication
CN111188102A
IPC
D01F8/14; D01F1/10; A61L27/18; A61L27/54; A61L27/58
CPC
A61L27/18; A61L27/54; A61L27/58; A61L2300/416; D01F1/10; D01F8/14; C08L67/04
Inventors
叶雷; 郭强