Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing flexible lipidosome

A technology of flexible liposomes and active substances, applied in the field of biomedicine, can solve problems such as difficult stability, complicated operation procedures, and difficulty in standardizing and expanding production

Pending Publication Date: 2020-05-26
ADVITA BIOTEC BEIJING CO LTD
View PDF9 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of these methods require high energy input, complex operating procedures, easy to bring impurities, difficult to standardize and expand production, and stability is also a problem that is difficult to solve

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing flexible lipidosome
  • Method for preparing flexible lipidosome
  • Method for preparing flexible lipidosome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Example 1: Preparation of stem cell-derived factor flexible liposomes and application dosage forms

[0076] Stem cell exosomes contain more than 300 biological factors, which exist in the culture medium and can be isolated and purified or directly used as active factors in the conditioned medium. They have the functions of whitening, beautifying the face, stimulating regeneration, and repairing wounds. It can be used in fields such as medicine or cosmetics.

[0077] The specific method for preparing the stem cell-derived factor flexible liposome and application dosage form is as follows:

[0078] 1. Preparation of Phospholipid Solution

[0079] Add 0.5g of soybean lecithin with 90w / v% phosphatidylcholine and 0.09g of Span 80 into 0.7mL of ethanol, wait for it to dissolve at room temperature, add 0.1g of vitamin E, stir at room temperature at 700rmp for 10 minutes, mix well, and obtaining a lipid solution;

[0080] 2. Preparation of Stem Cell-Derived Factor Solution ...

Embodiment 2

[0086] Embodiment 2: hepatitis B vaccine flexible liposome

[0087]Hepatitis B vaccine (Hepatitis B vaccine) is a purified hepatitis B surface antigen, a special drug used to prevent hepatitis B. After vaccination, the immune system can be stimulated to produce protective antibodies, which are present in human body fluids. Once the hepatitis B virus appears, the antibodies will act immediately to remove it and prevent infection without harming the liver, thus making the human body Have the immunity to prevent hepatitis B, so as to achieve the purpose of preventing hepatitis B infection. The common way of vaccination is intramuscular injection, and the positive rate of antibody can reach more than 96% after three injections. And the antibody effect is maintained at a high level. The use of transdermal technology can improve patient compliance without the pain of injections, and liposomes can encapsulate drugs to increase stability and sustained release. Improve the effectiven...

Embodiment 3

[0097] Example 3: Flexible liposomes of docetaxel and its topical use

[0098] Paclitaxel is one of the most widely used and effective antineoplastic agents obtained from natural sources. It has broad-spectrum antitumor activity, especially against ovarian cancer, breast cancer, non-small cell lung cancer, head and neck tumors, Kaposi's sarcoma, and malignant tumors of the urinary tract. This is a highly lipophilic substance with a Log P value of 3.96 and a water solubility of less than 0.01 mg / mL. In addition, the component lacks ionizable functional groups, so it cannot be used to increase its solubility by changing the pH, and the administration of paclitaxel faces great challenges. The inventors have confirmed through experiments that docetaxel has the highest solubility in caprylic triglyceride (Table 1), and this solvent is preferably used in the subsequent preparation of phospholipid solution.

[0099] Table 1. Solubility of docetaxel in different oils (n=3)

[0100]...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Parcel rateaaaaaaaaaa
Polydispersity indexaaaaaaaaaa
Login to View More

Abstract

The invention provides a simple and feasible method for preparing a flexible liposome. The preparation method comprises the following steps: dissolving phospholipid and a surfactant in a solvent, preparing a phospholipid solution, adding glycerol or propylene glycol into a buffer solution, preparing an aqueous phase solution, injecting the phospholipid solution into the aqueous phase solution, andstirring to obtain the flexible liposome. The flexible liposome obtained through the method is uniform in diameter and has good stability.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for flexible liposomes. Background technique [0002] In recent decades, various active pharmaceutical ingredients (APIs), such as heat-labile proteins, acid-labile drugs, enzyme-sensitive, highly lipophilic, hydrophilic, photosensitive drugs, and high-molecular-weight molecules, have been encapsulated within vesicles to carry. However, traditional liposomes are largely useless as transdermal delivery vehicles, and they usually accumulate in the stratum corneum, making it difficult to penetrate deeper regions or systemic circulation. [0003] Flexible liposomes (ELs), also known as deformable liposomes, deformable liposomes, and elastic liposomes, are biocompatible bilayer vesicle systems that can provide therapeutic, biochemical, and cosmetic purposes. Various means of drug delivery. This vesicle system has been developed for topical, nasal spray, oral and transdermal deli...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K9/127A61K47/10A61K47/24
CPCA61K9/127A61K9/0014A61K47/10A61K47/24
Inventor 关肇基张欣凤刘强刘奕
Owner ADVITA BIOTEC BEIJING CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com