Technical method for in-vitro culture of melanocyte stem cells for treating leucoderma

Abstract

The invention discloses a technical method for in-vitro culture of melanocyte stem cells in a process of treating leucoderma by hair follicle melanocyte stem cell transplantation. The technical methodcomprises the following steps: extracting and separating hair follicles, carrying out primary in-vitro culture on melanocyte stem cells, carrying out secondary in-vitro culture on the melanocyte stemcells, and carrying out culture to enhance the activity of the melanocytes, promote the transformation of the hair follicle melanocyte stem cells into the melanocytes and transplant the melanocyte stem cells. Through the hair follicle in-vitro culture technology, extracted and separated single-plant hair follicles are put into a special culture solution and are cultured, and through in-vitro culture and treatment of hair follicle melanocytes, leucoderma color restoration time is shortened, and the purpose of rapidly curing leucoderma is achieved.

Description

technical field [0001] The invention relates to the technical field of hair follicle transplantation, in particular to a technical method for in vitro culture of hair follicle melanocyte stem cells extracted during the treatment of vitiligo by hair follicle melanocyte stem cell transplantation. Background technique [0002] Vitiligo is an acquired skin and mucous membrane pigmentation disorder. The color recovery of skin lesions is mostly completed by the migration of melanocytes from other parts and the production of pigments. The repigmentation patterns of vitiligo skin lesions can be summarized as punctate, marginal, and uniform Type, mixed type, only the dot type recoloring is not easy to recur. The common clinical treatment methods for vitiligo mainly include traditional Chinese medicine treatment, corticosteroid and other western medicine treatment, photochemotherapy (308 excimer laser) and epidermal transplantation. The first two treatment measures vary from person to...

AI Technical Summary

Problems solved by technology

The common clinical treatment methods for vitiligo mainly include traditional Chinese medicine treatment, corticosteroid and other western medicine treatment, photochemotherapy (308 excimer laser) and epidermal transplantation. The first two treatment measures vary from person to person. Long, and the curative effect is uncertain, and some patients have relatively large side effects after taking the drug, such as nausea, vomiting, loss of appetite, etc. As for the treatment of corticosteroids, the curative effect is unstable and easy to relapse; one of the commonly used methods at present The epidermis transplantation of the skin, because the recoloring is uniform, unstable, easy to relapse; 308 excimer laser treatment cycle is long, the curative effect is average, side effects are large, even the recoloring effect is unstable, and the color is not uniform

In recent years, there have been reports of single hair follicle transplantation for the treatment of vitiligo, but because of many shortcomings in the treatment, it cannot be used clinically. In the dermis of vitiligo, because they did not jump out of the thinking mode of hair transplant surgery, they worried that the transplanted hair follicles would not survive, and ignored that the transplanted hair follicle melanocyte stem cells; There is melanin around, and there will be hair growth, but cutting off the lower 1 / 3 of the hair follicle will make it impossible to transport the hair follicle melanocyte stem cells along the outer root sheath, thus affecting the process of white spot recoloring

It can only achieve pigment regeneration around the implanted hair follicles, and requires a large number of hair follicle transplants to recolor. It cannot complete the treatment of moderate-area vitiligo, let alone meet the needs of large-area vitiligo treatment.

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