A kind of recombinant plasmid including astaxanthin synthase fusion gene, recombinant bacteria and application
A technology of astaxanthin synthase and recombinant plasmid, which is applied in application, genetic engineering, recombinant DNA technology, etc., to achieve the effect of low cost, simple and fast method, and high efficiency
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[0041] Camelina sativa (L.) germplasm material SC-N16, (see [Yuan Lixia, Hao Jingyun, Zhao Kui, et al. 2EMS mutagenesis of Camelina sativa (L.) to obtain 2S storage protein deletion mutants. Journal of North China Agricultural Science, 2016, 31(3):11-17]), provided by the Key Laboratory of Crop Genetics and Breeding, Shanxi Agricultural University. Sow camelina seeds in seedling pots with a diameter of 9 cm, 1 seed per pot; the substrate in the pot is humus: peat soil: perlite = 5:4:1 (volume ratio); the temperature in the greenhouse is 16-22°C, and the light source is Natural light, 60% relative humidity. When the plants grow to 10cm, topping is carried out to promote the growth and development of side branches. When the plant grows for about 70 days, it enters the flowering stage and prepares for transformation, and the plant is thoroughly watered the day before the transformation. A total of 20 plants (pots) were t...
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