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High-molecular copolymer probe

A polymer copolymer and block copolymer technology, applied in the field of polymer probes, can solve the problems of inability to perform quantitative detection or high-precision quantitative detection, impurity interference, etc., and achieve the effect of wide application range and high accuracy

Pending Publication Date: 2020-06-05
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that the existing detection of hydrogen peroxide is easily interfered by impurities, or some probes can reduce or even avoid the interference of impurities, but cannot perform quantitative detection or high-precision quantitative detection, the present invention provides a polymer Copolymer probe and preparation method thereof

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] A kind of polymer copolymer probe, its preparation method comprises the following preparation steps:

[0060] 1) Preparation of poly-9,9-dihexylfluorene (PFH) with repeat unit number n=3 by "controllable" Suzuki coupling polymerization 3 ), mixed bromo-modified poly-9,9-dihexylfluorene and N,N-dimethylaminoethyl methacrylate as a precursor in a molar ratio of 1:5 (0.01mmol:0.05mmol), Preparation of rod-wire block copolymer poly9,9-dihexylfluorene-b-polyN,N-dimethylaminoethyl methacrylate by atom transfer radical polymerization, denoted as PFH 3 -b-PDMAEMA 15 ;

[0061] 2) the PFH 3 -b-PDMAEMA 15 Dissolve in chloroform, under the protection of argon, add methyl iodide dropwise to the solution, stir until the reaction is complete, and then prepare a water-soluble cationic block copolymer containing polyfluorene group, denoted as PFH 3 -b-PDMAEMAI 15 .

[0062] Qualitative detection test:

[0063] Prepare 1mg / mL of PFH 3 -b-PDMAEMAI 15 aqueous solution, prepare t...

Embodiment 2

[0080] A kind of preparation method of macromolecular copolymer probe, described method comprises the following steps:

[0081] 1) Preparation of poly-9,9-diethylfluorene (PFE) with n=5 repeating units by "controllable" Suzuki coupling polymerization 5), the bromo-modified poly-9,9-diethylfluorene and N,N-dimethylaminoethyl methacrylate were mixed as a precursor in a molar ratio of 1:200 (0.01mmol: 2.00mmol) , to prepare rod-wire block copolymer poly9,9-diethylfluorene-b-polyN,N-dimethylaminoethyl methacrylate by means of atom transfer radical polymerization, denoted as PFE 5 -b-PDMAEMA 1000 ;

[0082] 2) PFE 5 -b-PDMAEMA 1000 Dissolve in tetrahydrofuran, under the protection of argon, add methyl iodide dropwise to the solution, stir until the reaction is complete, and then prepare a water-soluble cationic block copolymer containing polyfluorene group, denoted as PFE 5 -b-PDMAEMAI 1000 .

[0083] Qualitative detection test:

[0084] Prepare 1mg / mL of PFE 5 -b-PDMAEMAI...

Embodiment 3

[0089] A kind of preparation method of macromolecular copolymer probe, described method comprises the following steps:

[0090] 1) Prepare n=9 poly-9,9-dimethylfluorene (PFM) by "controllable" Suzuki coupling polymerization 9 ), the bromo-modified poly-9,9-dimethylfluorene and N,N-dimethylaminoethyl methacrylate were mixed as a precursor in a molar ratio of 1:100 (0.01mmol: 1.00mmol) , to prepare rod-wire block copolymer poly9,9-dimethylfluorene-b-polyN,N-dimethylaminoethyl methacrylate by means of atom transfer radical polymerization, denoted as PFM 9 -b-PDMAEMA 900 ;

[0091] 2) PFM 9 -b-PDMAEMA 900 Dissolve in N,N-dimethylformamide, add methyl iodide dropwise to the solution under the protection of argon, stir until the reaction is complete, and then prepare a water-soluble cationic block copolymer containing polyfluorene group , denoted as PFE 9 -b-PDMAEMAI 900 .

[0092] Qualitative detection test:

[0093] Prepare 1mg / mL of PFE 9 -b-PDMAEMAI 900 Aqueous soluti...

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Abstract

The invention relates to the technical field of polymer probes, in particular to a high-molecular copolymer probe. The probe is a water-soluble cationic block copolymer containing a polyfluorenyl group. A preparation method of the probe comprises: (1) polymerizing 7-bromo-2-boronic acid pinacol ester-9,9-disubstituted fluorene adopted as a monomer to prepare poly(9,9-disubstituted fluorene), performing bromination modification obtain brominated poly(9,9-disubstituted fluorene), and performing polymerization by adopting the brominated poly(9,9-disubstituted fluorene) and R'''-group ethyl methacrylate as precursors to obtain a rod-coil block copolymer that is poly(9,9-disubstituted fluorene)-b-poly(R'''-group ethyl methacrylate); and 2) subjecting the poly(9,9-disubstituted fluorene)-b-poly(R'''-group ethyl methacrylate) to cationization to obtain the water-soluble cationic block copolymer containing a polyfluorenyl group, named as poly(9,9-disubstituted fluorene)-b-poly(R'-group ethyl methacrylate). Under the combined action of the molecular probe and phenol substances, efficient targeting detection can be performed on hydrogen peroxide, and both detection precision and quantitativedetection are achieved.

Description

technical field [0001] The invention relates to the technical field of polymer probes, in particular to a polymer copolymer probe. Background technique [0002] Hydrogen peroxide is a common small molecule oxidant, which is also closely related to organisms. In organisms, hydrogen peroxide is mostly produced by the respiratory chain of mitochondria. In the respiratory chain, oxygen O 2 is reduced to O 2 , followed by O 2 It is transformed into hydrogen peroxide through the dismutation of superoxide dismutase (SODs), so hydrogen peroxide plays an important role in the process of biological cell signal transduction, it can not only produce other active oxygen, but also other active oxygen The product of the reaction. On the other hand, some human body components or secretions such as uric acid and glucose can also produce hydrogen peroxide under the action of corresponding enzymes. [0003] Current detection methods for hydrogen peroxide include redox-sensitive fluorescen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F293/00G01N21/64
CPCC08F293/005G01N21/6428G01N2021/6432C08F2438/01
Inventor 周密何泽坚吴娜李杰
Owner ZHEJIANG UNIV OF TECH
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