Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of microbes in screening of drugs for treating eye diseases

A technology of microorganisms and microbial infection, which is applied in the field of diagnosis and treatment of ophthalmic diseases, and can solve the problems that eye diseases cannot be checked, and the detection method of PM2.5 eye diseases has not been disclosed.

Pending Publication Date: 2020-06-09
ZHUHAI QLWEL BIO TECH LTD
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, eye diseases caused by other bacteria cannot be checked, only thyroid-related eye diseases can be checked, and the detection method for eye diseases caused by PM2.5 has not been disclosed

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of microbes in screening of drugs for treating eye diseases
  • Application of microbes in screening of drugs for treating eye diseases
  • Application of microbes in screening of drugs for treating eye diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] The preparation of embodiment 1 culture medium model

[0090] The specific operation steps are: pour bacterial culture medium into a petri dish, purchased from China Guangzhou Huankai Microbiology Co., Ltd., the composition of the bacterial culture medium is as follows: each liter of double pure water contains 5g of peptone, 3g of beef extract, and 5g of sodium chloride , agar 15g and MnSO 4 5 mg. Sterilize the Petri dish containing the culture medium in an autoclave at 121°C for 30 min and cool down. Each flask was inoculated with approximately 1*10 7 A Bacillus megaterium was cultured in an incubator at 37°C for 24 hours to prepare a culture medium model.

Embodiment 2

[0091] Embodiment 2 culture medium model is used for drug screening

[0092] In order to test whether antibiotics can control the growth of Bacillus megaterium in vitro, we conducted an antibiotic sensitivity screening test in the culture medium model prepared in Example 1. The sensitivity of Bacillus megaterium to five major antimicrobial agents, ampicillin, vancomycin, neomycin, metronidazole and tetracycline, was examined using the minimum inhibitory concentration (MIC) method. Add different concentrations (10 -1 、10 -2 、10 -3 、10 -4 、10 -5 mg / mL) of ampicillin, vancomycin, neomycin, metronidazole, tetracycline, the above-mentioned antibacterial agents are all purchased from Sigma Corporation of the United States, and the experimental results are as follows: figure 1 As shown, by comparing with blank control and group inspection, it is found that Bacillus megaterium has the highest sensitivity to neomycin, followed by vancomycin, and metronidazole has the lowest effici...

Embodiment 3

[0093] Example 3 Preparation of aqueous humor / vitreous humor culture model and drug screening

[0094] Cooked meat medium was purchased from Guangzhou Huankai Microbiology Co., Ltd., China, and its ingredients were: Lab-Lemco' powder 5g / L, peptone 30g / L, yeast extract 5g / L, NaH 2 PO 45g / L, glucose 3g / L, soluble starch 2g / L, no antibiotics. Add 6ml of cooked meat culture medium and sterilized dried beef grains into a 15ml glass tube, and add 1.5ml of liquid paraffin to seal it as a culture system. All tubes were then sterilized at 121°C for 30 minutes and cooled. Aqueous humor or vitreous humor (with and without drug) was injected into the glass tube above, and then cultured in a ZQTY-70F incubator at 37°C for 72 hours with shaking (200rpm) to prepare the aqueous humor / vitreous humor culture model. Cultures without aqueous humor or vitreous humor samples were used as negative controls, and E. coli cultures were used as positive controls. All cultures were then Gram-stained ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

On the research basis that microbes of specific species are present in eyes of patients with different eye diseases, the invention discloses a method for establishing a model, and the method comprisesthe step of infecting a model carrier with the microbes. The model prepared by the method can be used for screening drugs for the eye diseases, and the eye diseases include senile macular degeneration, the behcet disease, the Vogt-Koyanagi-Harada syndrome, uveitis, retinopathy, xerophthalmia, sympathetic ophthalmia, trachoma, cataract, conjunctivitis, chalazion, glaucoma, muscae volitantes and the like.

Description

technical field [0001] The invention belongs to the technical field of diagnosis and treatment of ophthalmic diseases, and in particular relates to an application of microorganisms in drug screening for ocular diseases. Background technique [0002] Eyes are the windows of the soul. Eyes are very important to everyone. People use their eyes every day, but at the same time, their eyes are very fragile. It is easy to cause eye discomfort or lesions due to various factors . The more common ones are conjunctivitis, dry eye, etc., and the more serious eye diseases include cataract, age-related macular degeneration, glaucoma, Behcet's disease, Vogt-Koyanagi-Harada syndrome, uveitis, etc. No matter what kind of eye disease, it will seriously affect people's normal life. [0003] For example, age-related macular degeneration, also known as age-related macular degeneration (AMD), is an aging change in the structure of the macula. The main manifestation is that the ability of retin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74A61K35/68A61K36/06A61K35/76A61K49/00C12N1/20C12N1/14C12N1/10C12N7/00
CPCA61K35/74A61K35/68A61K36/06A61K35/76A61K49/0008C12N1/20C12N1/14C12N1/10C12N7/00C12Q1/025A61K31/43A61K31/7036A61K31/4164A61K31/65G01N33/5088G01N2800/16G01N2500/10A61K35/742A61K35/747A61K35/744A61K2035/115Y02A50/30C12Q1/18
Inventor 魏来
Owner ZHUHAI QLWEL BIO TECH LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com