Non-decalcified bone tissue plastic embedding ultrathin flaking method

A plastic bag and decalcified bone technology, applied in the biological field, can solve the problems of limiting bone metabolic diseases, large thickness of bone grinding slices, and restricting tissue observation, etc., to achieve clear cell morphology, reduce slice thickness, and clear cell tissue structure Effect

A plastic bag and decalcified bone technology, applied in the biological field, can solve the problems of limiting bone metabolic diseases, large thickness of bone grinding slices, and restricting tissue observation, etc., to achieve clear cell morphology, reduce slice thickness, and clear cell tissue structure Effect

CN111257084APending Publication Date: 2020-06-09JIANGSU PROVINCE HOSPITAL THE FIRST AFFILIATED HOSPITAL WITH NANJING MEDICAL UNIV
3 Cites 4 Cited by

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Non-decalcified bone tissue plastic embedding ultrathin flaking method
  • Non-decalcified bone tissue plastic embedding ultrathin flaking method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] This plastic embedding tablet method adopts the following process steps under 4 degrees,

[0024] 1. Fixation: fix tissue specimens with mercuric chloride, potassium dichromate and formaldehyde fixative (PCF), specimen size: diameter: 0.5cm, length: 1.5cm~2.5cm. Fixed time 60h~72h, temperature: 4 degrees, pressure: 0.1MPa.

[0025] 2. Dehydration: Gradient dehydration, tissue specimen dehydration, dehydration with volume fraction 60% ethanol 120min, 70% ethanol dehydration 120min, 80% ethanol dehydration 120min, 95% ethanol dehydration 120min, 100% ethanol dehydration 120min (2 times), pure Acetone was used to absorb residual ethanol in tissue samples for 15 minutes, and all reagents were pre-cooled at 4 degrees. The above are temperature: 4 degrees, pressure: 0.1MPa.

[0026] 3. Soaking: use hydroxyethyl methacrylate (HEMA) as the soaking liquid, soaking temperature: 4 degrees, pressure: 0.1 MPa, time: 48-60 hours; after soaking and dehydrating the tissue specimens, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a non-decalcified bone tissue plastic embedding ultrathin flaking method, which comprises the steps of fixing, dehydrating, soaking, embedding, slicing and dyeing in sequence,and the steps of fixing, dehydrating, soaking and embedding are all carried out at 4 DEG C. Compared with a traditional plastic embedding flaking method, the hardness of the prepared plastic embeddingblock is reduced, the thickness of a slice is reduced to 2-5 microns from 50-70 microns of the thickness of a traditional plastic abrasive disc, the bone tissue flaking quality is greatly improved, and the bone tissue structure is displayed more clearly.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a non-decalcified bone tissue plastic-embedded ultra-thin sheet-making method. Background technique [0002] Morphology and histology are one of the important means to study the behavior of bone tissue and the process of bone repair and reconstruction. The conventional paraffin embedding technique must decalcify the bone tissue, which severely damages the inherent structure of the bone tissue, resulting in the inability to distinguish mineralized bone from uncalcified osteoid, and cannot accurately evaluate the new bone formation, especially Bone tissue specimens with implants need to be removed for paraffin tissue embedding and sectioning, so it is impossible to accurately observe the combination of implants and surrounding new bone. [0003] Plastic embedding refers to the use of a mixed reagent of hydroxyethyl methacrylate, polyethylene glycol-400, N-N dimethylaniline...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
09 Jun 2020
Publication
CN111257084A
IPC
G01N1/28; G01N1/30; G01N1/36
CPC
G01N1/28; G01N1/286; G01N1/30; G01N1/36; G01N2001/2873; G01N2001/302
Inventors
朱敬凤; 邢昌赢