Non-decalcified bone tissue plastic embedding ultrathin flaking method

Abstract

The invention discloses a non-decalcified bone tissue plastic embedding ultrathin flaking method, which comprises the steps of fixing, dehydrating, soaking, embedding, slicing and dyeing in sequence,and the steps of fixing, dehydrating, soaking and embedding are all carried out at 4 DEG C. Compared with a traditional plastic embedding flaking method, the hardness of the prepared plastic embeddingblock is reduced, the thickness of a slice is reduced to 2-5 microns from 50-70 microns of the thickness of a traditional plastic abrasive disc, the bone tissue flaking quality is greatly improved, and the bone tissue structure is displayed more clearly.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a non-decalcified bone tissue plastic-embedded ultra-thin sheet-making method. Background technique [0002] Morphology and histology are one of the important means to study the behavior of bone tissue and the process of bone repair and reconstruction. The conventional paraffin embedding technique must decalcify the bone tissue, which severely damages the inherent structure of the bone tissue, resulting in the inability to distinguish mineralized bone from uncalcified osteoid, and cannot accurately evaluate the new bone formation, especially Bone tissue specimens with implants need to be removed for paraffin tissue embedding and sectioning, so it is impossible to accurately observe the combination of implants and surrounding new bone. [0003] Plastic embedding refers to the use of a mixed reagent of hydroxyethyl methacrylate, polyethylene glycol-400, N-N dimethylaniline...

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Problems solved by technology

[0004] CN101430261A discloses a plastic embedding method for making slices, but the thickness of slices obtained by this method is 50-70 μm, which seriously restricts the observation of tissues, and has been an insoluble difficulty in the field of histology for a long time

For patients who urgently need to observe the bone tissue structure and bone cell morphology, the thickness of the bone grinding slice is too large, the staining is difficult, and the observation effect is too poor, which limits the observation and research of bone metabolic diseases.

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