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Medicine for preventing and treating alcoholic liver diseases and extraction method

A technology of alcoholic liver disease and extraction method, which is applied in the field of liver disease drugs, can solve the problems of no specific drugs and achieve the effect of liver disease relief

Inactive Publication Date: 2020-06-19
GANSU UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] To sum up, the problem existing in the prior art is: at present, although there are many clinical drugs for the treatment of alcoholic liver disease, including anti-oxidation and anti-inflammatory drugs, there is no recognized specific drug

Method used

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  • Medicine for preventing and treating alcoholic liver diseases and extraction method
  • Medicine for preventing and treating alcoholic liver diseases and extraction method
  • Medicine for preventing and treating alcoholic liver diseases and extraction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] 1. Materials

[0062] 1.1 Reagents

[0063] 75 male Kunming mice (18-22g, 4 weeks old); silymarin (5g, Dalian Meilun Company, batch number 50707A); Red Star Erguotou (56 degrees); 18112604); ALT, AST kits, etc.

[0064] 1.2 Instruments

[0065] Powder machine; ultrasonic instrument; rotary evaporator; freeze dryer; refrigerator; pipette gun, microplate reader, etc.

[0066] 2. Pre-test method

[0067] (1) Drug extraction

[0068] Break Gentiana decoction pieces into coarse powder (300g), ultrasonically extract 3 times with 70% ethanol (solid-liquid ratio 1:10), each time for 30min, filter under reduced pressure, combine filtrates, concentrate and evaporate to dryness to obtain alcohol extract, freeze-dry 107.36g of powder was obtained, and the extraction rate was 35.79%.

[0069] (2) The volume of 56% alcohol gavage is 10ml / kg (equivalent to the alcohol dose of 4.8g / kg), and the treatment of each group is as follows:

[0070] 1) Normal group (K, 10 rats in total)...

Embodiment 2

[0083] The method for extracting the medicine for preventing and treating alcoholic liver disease provided by the embodiments of the present invention comprises:

[0084] (1) Break Gentiana into coarse powder, ultrasonically extract 3 times with 70% ethanol (solid-liquid ratio 1:10), each time for 20 min, filter under reduced pressure, combine filtrates, concentrate and evaporate to dryness to obtain alcohol extract, freeze-dry.

[0085] (2) The mice were divided into normal group, model group, positive group (silymarin) and Gentiana group (high, middle and low dose groups), and 56% alcohol (10ml / kg) was intragastrically administered to establish the model, and the test period was 2 weeks.

[0086] (3) Indicator detection:

[0087] 1) HE staining (liver, heart, spleen, lung, kidney, brain tissue);

[0088] 2) Oil red staining (liver tissue);

[0089] 3) The kit detects serum ALT, AST and other liver function indicators, as well as liver tissue lipid content and oxidative str...

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Abstract

The invention belongs to the technical field of medicines for treating liver diseases, and discloses a medicine for preventing and treating alcoholic liver diseases and an extraction method. The extraction method comprises the following steps: grinding radix gentianae macrophyllae into coarse powder, carrying out ultrasonic extraction for three times by using 70% ethanol for 20 minutes each time,carrying out reduced pressure filtration, combining filtrates, performing concentration and evaporation drying to obtain an alcohol extract, and carrying out freeze drying. Mice are divided into a normal group, a model group, a positive group, an alcohol+gentiana macrophylla group and a gentiana macrophylla group, 56% alcohol is used for gavage modeling, and the test period is 2 weeks; and index detection is performed: HE staining and oil red staining are performed, and a kit detects serum ALT, AST and other liver function indexes, a liver tissue lipid content and oxidative stress related indexes. According to the invention, the processes of ultrasonic extraction, rotary evaporation, freeze drying and other medicine treatment processes are carried out below 45 DEG C; and experiments show that the radix gentianae macrophyllae alcohol extract has a certain relieving effect on liver diseases caused by alcohol and has no obvious damage to other organs.

Description

technical field [0001] The invention belongs to the technical field of liver disease drugs, in particular to a drug and an extraction method for preventing and treating alcoholic liver disease. Background technique [0002] Currently, alcoholic liver disease (ALD) is a worldwide chronic disease and one of the leading causes of death from chronic liver disease. ALD is a liver disease caused by long-term excessive drinking. It usually manifests as fatty liver in the initial stage, and can develop into alcoholic hepatitis, liver fibrosis, cirrhosis, and even liver cancer. Alcohol abstinence is the most basic treatment measure, and drug treatment is generally more meaningful no later than the hepatitis stage, but there is no specific drug approved to treat liver fibrosis, let alone liver cirrhosis or liver cancer, the best treatment option for a liver transplant. Therefore, how to develop safer and more effective drugs for the prevention and treatment of ALD is necessary. Fin...

Claims

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Application Information

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IPC IPC(8): A61K36/51A61P1/16A61P39/02
CPCA61K36/51A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61P1/16A61P39/02
Inventor 晋玲曹后康张可锋席少阳林丽马晓辉刘立
Owner GANSU UNIV OF CHINESE MEDICINE
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