Application of pleurotus ostreatus polysaccharide selenoside-III anticancer active component in preparation of anti-prostatic cancer drug

An anti-prostate cancer, oyster mushroom polysaccharide technology, applied in the field of medicine, can solve problems such as inability to cure tumors, and achieve good anti-cancer activity and excellent anti-cancer activity

Active Publication Date: 2021-01-19
湖南万臻生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most prostate cancer patients have no obvious clinical symptoms, which leads to many patients being discovered at an advanced stage. Most advanced tumors have more or less metastatic cell clusters. Local treatments such as surgery and radiotherapy cannot cure the tumor theoretically. It can only achieve the purpose of diagnosis, staging and tumor reduction

Method used

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  • Application of pleurotus ostreatus polysaccharide selenoside-III anticancer active component in preparation of anti-prostatic cancer drug
  • Application of pleurotus ostreatus polysaccharide selenoside-III anticancer active component in preparation of anti-prostatic cancer drug
  • Application of pleurotus ostreatus polysaccharide selenoside-III anticancer active component in preparation of anti-prostatic cancer drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] (1) Preparation of crude selenium polysaccharide

[0060] Take 15 g of selenium-enriched Pleurotus ostreatus powder (provided by Hunan Wanzhen Biotechnology Co., Ltd.) into a round bottom flask, add 120 mL of 80% ethanol-water solution, stir and reflux at 65° C. for 3 h, filter with suction to obtain a filter cake, and dry.

[0061] Water extraction: Take 10 g of the filter cake in a flask, add 300 mL of ultrapure water, stir at 85° C. for 3 h, centrifuge, and concentrate the supernatant to obtain a viscous liquid.

[0062] Alcohol precipitation: drop 4 times the amount of absolute ethanol into the viscous liquid, place it at 4°C for 24 hours, and centrifuge to obtain a precipitate.

[0063] Deproteinization: dissolve the precipitate in 100mL of water, add 1 / 4 volume of sevage reagent, centrifuge to get the supernatant, repeat 6 times, dialyze with a dialysis bag, freeze-dry to obtain crude polysaccharide (also known as Pleurotus ostreatus crude polysaccharide) or crud...

Embodiment 2

[0074] Pharmacodynamic experiment of polysaccharide selenoside-Ⅲ (obtained in Example 1) on human prostate cancer cells

[0075] When the PC-3 cell density reaches 80-90%, digest with trypsin until the cells become round, and then add cell culture medium to stop the digestion. The cell suspension was appropriately diluted and counted with a hemocytometer, and the plate (96-well plate) was seeded at a density of 8000 cells per well, and the 96-well plate was placed in a cell incubator and incubated for 24 hours.

[0076] Afterwards, the culture medium in the 96-well plate was replaced with serum-free cell culture medium, and incubated in a cell culture incubator for 24 hours.

[0077] The previous serum-free medium was replaced with cell culture medium containing different concentrations of polysaccharide selenoside-III (0 μg / mL, 50 μg / mL, 100 μg / mL, 200 μg / mL, 400 μg / mL, 600 μg / mL) and incubated for 24 h. And the cell-free and simple cell culture medium was used as the negati...

Embodiment 3

[0093] Drug effect test of polysaccharide selenoside-Ⅲ (embodiment 1) on normal cells

[0094] When the density of normal human liver cells reaches 80-90%, digest with trypsin until the cells become round, and then add cell culture medium to stop the digestion. The cell suspension was appropriately diluted and counted with a hemocytometer, and the plate (96-well plate) was seeded at a density of 8000 cells per well, and the 96-well plate was placed in a cell incubator and incubated for 24 hours.

[0095] Afterwards, the culture medium in the 96-well plate was replaced with serum-free cell culture medium, and incubated in a cell culture incubator for 24 hours.

[0096] The previous serum-free medium was replaced with cell culture medium containing different concentrations of polysaccharide selenoside-III (0 μg / mL, 50 μg / mL, 100 μg / mL, 200 μg / mL, 400 μg / mL, 600 μg / mL) and incubated for 24 h. And the cell-free and simple cell culture medium was used as the negative control group...

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Abstract

The invention belongs to the technical field of medicine development, and particularly discloses pleurotus ostreatus polysaccharide selenoside-III. The invention discloses a pleurotus ostreatus polysaccharide selenoside-III anticancer drug with prostatic cancer resistance, and discloses a preparation method thereof. The pleurotus ostreatus polysaccharide selenoside-III anticancer drug can specifically kill prostatic cancer cells, and can promote metabolism and proliferation of normal cells. A feasible scheme is provided for treating the prostatic cancer.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to an anticancer active ingredient of oyster mushroom polysaccharide selenoside-III with high anticancer efficacy and low toxicity and side effects. [0002] technical background [0003] Prostate cancer is one of the most common malignant tumors in men, and its incidence is related to regions and races. In the West, prostate cancer is still the most common malignant tumor in men, and its incidence ranks first. my country is in an area with a relatively low incidence of prostate cancer, but in recent years, with the development of my country's social economy and changes in people's eating habits, the tumor spectrum has gradually changed, and the incidence of prostate cancer has increased year by year, which has seriously threatened the physical and mental health of men. healthy. It is urgent to study anticancer drugs with specific effects on prostate cancer. [0004] Most prostate ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/04A61K31/715A61P35/00A61P13/08C08B37/00
CPCA61K31/715A61K33/04A61P35/00A61P13/08C08B37/0003C08B37/006A61K2300/00Y02A50/30
Inventor 钟世安张云山刘慧张卓敏邓志伟沈剑
Owner 湖南万臻生物科技有限公司
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